Magnolol Pretreatment Attenuates Heat Stress-induced IEC-6 Cell Injury

Overview

Journal J Zhejiang Univ Sci B

Specialties Biology
General Medicine

Date 2016 Jun 4

PMID 27256675

Citations 5

Authors

Chen Mei

Sha-sha He

Peng Yin

Lei Xu

Ya-Ran Shi

Xiao-Hong Yu

An Lyu

Feng-Hua Liu

Lin-Shu Jiang

Affiliations

Soon will be listed here.

Abstract

Objective: Heat stress (HS) is an important environmental stressor that adversely influences livestock during the summer. The aim of this study was to investigate whether magnolol protects against HS-induced intestinal epithelial cell injury.

Materials And Methods: An intestinal epithelial cell line (IEC-6) was subjected to HS at 42 °C, with and without magnolol pretreatment. Cell injury was detected by monitoring lactate dehydrogenase (LDH) release. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was used to assess cell proliferation and viability, including identifying effective concentrations of magnolol. Flow cytometry confirmed G1-phase cell-cycle arrest and its alleviation by magnolol. Active DNA synthesis was measured by incorporation of nucleic acid 5-ethynyl-2'-deoxyuridine (EdU). G1-phase cell-cycle-related gene expression was assessed by real-time reverse transcription polymerase chain reaction (RT-PCR) and levels of G1-phase-related proteins by Western blotting.

Results: HS induced IEC-6 cell injury and decreased cell viability, as demonstrated by data from LDH and MTS assays, respectively. Based on a number of criteria, IEC-6 cells subjected to HS were arrested in the G1 phase of the cell cycle. Magnolol pretreatment decreased HS-induced cell injury through relief of this cell-cycle arrest.

Conclusions: Magnolol pretreatment attenuates HS-induced injury in IEC-6 cells. Magnolol is potentially promising as a protective strategy for HS in livestock.

Citing Articles

Magnolol additive improves growth performance of Linwu ducklings by modulating antioxidative status.

Lin Q, Liu Y, Peng S, Liu C, Lv T, Liao L PLoS One. 2021; 16(12):e0259896.

PMID: 34972101 PMC: 8719751. DOI: 10.1371/journal.pone.0259896.

Isolation, culture, and identification of duck intestinal epithelial cells and oxidative stress model constructed.

Zhang H, Chen F, Liang Z, Wu Y, Pi J In Vitro Cell Dev Biol Anim. 2019; 55(9):733-740.

PMID: 31385166 DOI: 10.1007/s11626-019-00388-7.

Insights on the Multifunctional Activities of Magnolol.

Zhang J, Chen Z, Huang X, Shi W, Zhang R, Chen M Biomed Res Int. 2019; 2019:1847130.

PMID: 31240205 PMC: 6556366. DOI: 10.1155/2019/1847130.

Magnolol additive as a replacer of antibiotic enhances the growth performance of ducks.

Lin Q, Zhao J, Xie K, Wang Y, Hu G, Jiang G Anim Nutr. 2018; 3(2):132-138.

PMID: 29767073 PMC: 5941113. DOI: 10.1016/j.aninu.2017.03.004.

Heat stress induces intestinal injury through lysosome- and mitochondria-dependent pathway in vivo and in vitro.

Yi G, Li L, Luo M, He X, Zou Z, Gu Z Oncotarget. 2017; 8(25):40741-40755.

PMID: 28380464 PMC: 5522251. DOI: 10.18632/oncotarget.16580.

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