Characterization of Cholinesterase Molecular Forms in the Mucosal Cells Along the Intestine of the Chicken

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 1989 Jan 23

PMID 2725480

Citations 3

Authors

J P Sine

R Ferrand

B Colas

Affiliations

Soon will be listed here.

Abstract

The presence of a butyrylcholinesterase (BuChE, EC 3.1.1.8) in the musocal cells of the chicken intestine was demonstrated by histochemical and biochemical methods. The study of its distribution, along the intestine from duodenum to rectum, showed that the jejuno-ileum possesses the highest activity. Sucrose gradient centrifugation revealed, in all intestinal areas, two globular forms with sedimentation coefficients of 4.3 S (G1 form) and 10.8 S (G4 form). The presence of Triton X-100 in the preparations did not modify the sedimentation profiles of these two forms which can be considered as soluble BuChE. The ratio of G1/G4-forms progressively decreases along the intestine from duodenum to rectum indicating a predominance of the G4 form in the areas where the activity is low. Our results are discussed in relation to other studies of globular forms of chicken BuChE.

Citing Articles

Embryonic and post-natal changes in activity and molecular forms of mucosal cell butyrylcholinesterase in chicken intestine.

Sine J, Ferrand R, Colas B Mol Cell Biochem. 1990; 96(2):143-51.

PMID: 2274047 DOI: 10.1007/BF00420906.

Sine J, Ferrand R, Colas B Mol Cell Biochem. 1991; 103(1):15-21.

PMID: 1857343 DOI: 10.1007/BF00229589.

Human intestine epithelial cell acetyl- and butyrylcholinesterase.

Sine J, Ferrand R, Cloarec D, Lehur P, Colas B Mol Cell Biochem. 1991; 108(2):145-9.

PMID: 1779960 DOI: 10.1007/BF00233119.

References

Allemand P, Bon S, Massoulie J, Vigny M . The quaternary structure of chicken acetylcholinesterase and butyrylcholinesterase; effect of collagenase and trypsin. J Neurochem. 1981; 36(3):860-7. DOI: 10.1111/j.1471-4159.1981.tb01673.x. View

Silman I, Di Giamberardino L, Lyles L, Couraud J, Barnard E . Parallel regulation of acetylcholinesterase and pseudocholinesterase in normal, denervated and dystrophic chicken skeletal muscle. Nature. 1979; 280(5718):160-2. DOI: 10.1038/280160a0. View

Sine J, Colas B . Soluble form of acetylcholinesterase from rabbit enterocytes: comparison of its molecular properties with those of the plasma membrane species. Biochimie. 1987; 69(1):75-80. DOI: 10.1016/0300-9084(87)90274-4. View

Massoulie J, Bon S, Vigny M . The polymorphism of cholinesterase in vertebrates. Neurochem Int. 2010; 2C:161-84. DOI: 10.1016/0197-0186(80)90022-4. View

MAROUX S, Louvard D, Baratti J . The aminopeptidase from hog intestinal brush border. Biochim Biophys Acta. 1973; 321(1):282-95. DOI: 10.1016/0005-2744(73)90083-1. View

Karnovsky M, ROOTS L . A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES. J Histochem Cytochem. 1964; 12:219-21. DOI: 10.1177/12.3.219. View

Lyles J, Barnard E, Silman I . Changes in the levels and forms of cholinesterases in the blood plasma of normal and dystrophic chickens. J Neurochem. 1980; 34(4):978-87. DOI: 10.1111/j.1471-4159.1980.tb09674.x. View

Bon S, Massoulie J . Collagen-tailed and hydrophobic components of acetylcholinesterase in Torpedo marmorata electric organ. Proc Natl Acad Sci U S A. 1980; 77(8):4464-8. PMC: 349864. DOI: 10.1073/pnas.77.8.4464. View

Gennari K, Brodbeck U . Molecular forms of acetylcholinesterase from human caudate nucleus: comparison of salt-soluble and detergent-soluble tetrameric enzyme species. J Neurochem. 1985; 44(3):697-704. DOI: 10.1111/j.1471-4159.1985.tb12871.x. View

10.

Grassi J, Vigny M, Massoulie J . Molecular forms of acetylcholinesterase in bovine caudate nucleus and superior cervical ganglion: solubility properties and hydrophobic character. J Neurochem. 1982; 38(2):457-69. DOI: 10.1111/j.1471-4159.1982.tb08651.x. View

11.

Lyles J, Silman I, Di Giamberardino L, Couraud J, Barnard E . Comparison of the molecular forms of the cholinesterases in tissues of normal and dystrophic chickens. J Neurochem. 1982; 38(4):1007-21. DOI: 10.1111/j.1471-4159.1982.tb05342.x. View

12.

Vigny M, Gisiger V, Massoulie J . "Nonspecific" cholinesterase and acetylcholinesterase in rat tissues: molecular forms, structural and catalytic properties, and significance of the two enzyme systems. Proc Natl Acad Sci U S A. 1978; 75(6):2588-92. PMC: 392607. DOI: 10.1073/pnas.75.6.2588. View

13.

Colas B, MAROUX S . Simultaneous isolation of brush border and basolateral membrane from rabbit enterocytes. Presence of brush border hydrolases in the basolateral membrane of rabbit enterocytes. Biochim Biophys Acta. 1980; 600(2):406-20. DOI: 10.1016/0005-2736(80)90444-7. View

14.

Brimijoin S . Molecular forms of acetylcholinesterase in brain, nerve and muscle: nature, localization and dynamics. Prog Neurobiol. 1983; 21(4):291-322. DOI: 10.1016/0301-0082(83)90015-1. View

15.

Massoulie J, Bon S . The molecular forms of cholinesterase and acetylcholinesterase in vertebrates. Annu Rev Neurosci. 1982; 5:57-106. DOI: 10.1146/annurev.ne.05.030182.000421. View

16.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

17.

Sine J, Colas B . Presence and characterization of acetylcholinesterase in brush-border and basolateral membranes of rabbit enterocytes. Biochim Biophys Acta. 1985; 817(1):190-2. DOI: 10.1016/0005-2736(85)90083-5. View

18.

Lyles J, Silman I, Barnard E . Developmental changes in levels and forms of cholinesterases in muscles of normal and dystrophic chickens. J Neurochem. 1979; 33(3):727-38. DOI: 10.1111/j.1471-4159.1979.tb05218.x. View

19.

Sine J, Ferrand R, Colas B . Acetylcholinesterase and butyrylcholinesterase in the gut mucosal cells of various mammal species: distribution along the intestine and molecular forms. Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988; 91(2):597-602. DOI: 10.1016/0742-8413(88)90084-9. View

20.

Toutant J, Massoulie J, Bon S . Polymorphism of pseudocholinesterase in Torpedo marmorata tissues: comparative study of the catalytic and molecular properties of this enzyme with acetylcholinesterase. J Neurochem. 1985; 44(2):580-92. DOI: 10.1111/j.1471-4159.1985.tb05452.x. View