Semi-automated Microplate Monitoring of Protein Polymerization and Aggregation

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 2016 Jun 3

PMID 27251433

Citations 4

Authors

Veronica M Garcia

Veronica W Rowlett

William Margolin

Kevin A Morano

Affiliations

Soon will be listed here.

Abstract

Static light scattering (SLS) is a commonly used technique for monitoring dynamics of high molecular weight protein complexes such as protein oligomers or aggregates. However, traditional methods are limited to testing a single condition and typically require large amounts of protein and specialized equipment. We show that a standard microplate reader can be used to characterize the molecular dynamics of different types of protein complexes, with the multiple advantages of microscale experimental volumes, semi-automated protocols and highly parallel processing.

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