Identification of Secreted Exoproteome Fingerprints of Highly-Virulent and Non-Virulent Staphylococcus Aureus Strains

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2016 Jun 1

PMID 27242969

Citations 7

Authors

Emilia Bonar

Iwona Wojcik

Urszula Jankowska

Sylwia Kedracka-Krok

Michal Bukowski

Klaudia Polakowska

Marcin W Lis

Maja Kosecka-Strojek

Artur J Sabat

Grzegorz Dubin

Alexander W Friedrich

Jacek Miedzobrodzki

Adam Dubin

Benedykt Wladyka

Affiliations

Soon will be listed here.

Abstract

Staphylococcus aureus is a commensal inhabitant of skin and mucous membranes in nose vestibule but also an important opportunistic pathogen of humans and livestock. The extracellular proteome as a whole constitutes its major virulence determinant; however, the involvement of particular proteins is still relatively poorly understood. In this study, we compared the extracellular proteomes of poultry-derived S. aureus strains exhibiting a virulent (VIR) and non-virulent (NVIR) phenotype in a chicken embryo experimental infection model with the aim to identify proteomic signatures associated with the particular phenotypes. Despite significant heterogeneity within the analyzed proteomes, we identified alpha-haemolysin and bifunctional autolysin as indicators of virulence, whereas glutamylendopeptidase production was characteristic for non-virulent strains. Staphopain C (StpC) was identified in both the VIR and NVIR proteomes and the latter fact contradicted previous findings suggesting its involvement in virulence. By supplementing NVIR, StpC-negative strains with StpC, and comparing the virulence of parental and supplemented strains, we demonstrated that staphopain C alone does not affect staphylococcal virulence in a chicken embryo model.

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