Differential Proteomics Analysis of Colonic Tissues in Patients of Slow Transit Constipation

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2016 May 31

PMID 27239471

Citations 1

Authors

Songlin Wan

Weicheng Liu

Cuiping Tian

Xianghai Ren

Zhao Ding

Qun Qian

Congqing Jiang

Yunhua Wu

Affiliations

Soon will be listed here.

Abstract

Objective. To investigate and screen the different expression of proteins in STC and normal group with a comparative proteomic approach. Methods. Two-dimensional electrophoresis was applied to separate the proteins in specimens from both 5 STC patients and 5 normal controls. The proteins with statistically significant differential expression between two groups were identified by computer aided image analysis and matrix assisted laser desorption ionization tandem time of flight mass spectrometry (MALDI-TOF-MS). Results. A total of 239 protein spots were identified in the average gel of the normal control and 215 in patients with STC. A total of 197 protein spots were matched and the mean matching rate was 82%. There were 14 protein spots which were expressed with statistically significant differences from others. Of those 14 protein spots, the expression of 12 spots increased markedly, while that of 2 spots decreased significantly. Conclusion. The proteomics expression in colonic specimens of STC patients is statistically significantly different from that of normal control, which may be associated with the pathogenesis of STC.

Citing Articles

Characterization of Circular RNA Expression Profiles in Colon Specimens of Patients with Slow Transit Constipation.

Xi C, Hong Y, Chen B, Xie X, Liu W, Qian Q Dis Markers. 2022; 2022:3653363.

PMID: 35730015 PMC: 9206760. DOI: 10.1155/2022/3653363.

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