Different Effects of Metarhizium Anisopliae Strains IMI330189 and IBC200614 on Enzymes Activities and Hemocytes of Locusta Migratoria L

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 May 27

PMID 27227835

Citations 7

Authors

Guangchun Cao

Miao Jia

Xia Zhao

Lei Wang

Xiongbing Tu

Guangjun Wang

Xiangqun Nong

Zehua Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Metarhizium is an important class of entomopathogenic fungi in the biocontrol of insects, but its virulence is affected by insect immunity. To clarify the mechanism in virulence of Metarhizium, we compared the immunological differences in Locusta migratoria L. when exposed to two strains of Metarhizium anisopliae (Ma).

Results: The virulence of Ma IMI330189 was significantly higher than that of Ma IBC200614 to locust, and IMI330189 overcame the hemocytes and began destroying the hemocytes of locust at 72 h after spray, while locust is immune to IBC200614. IMI330189 could overcome the humoral immunity of locust by inhibiting the activities of phenol oxidase (PO), esterases, multi-function oxidases (MFOs) and acetylcholinesterases in locust while increasing the activities of glutathione-S-transferases (GSTs), catalase and aryl-acylamidase (AA). However IBC200614 inhibit the activities of GSTs and AA in locust and increase the activities of MFOs, PO, superoxide dismutase, peroxidase and chitinase in locust. The changes of enzymes activities in period of infection showed that the time period between the 2nd and the 5th day after spray is critical in the pathogenic process.

Conclusion: These results found the phenomenon that Ma initiatively broke host hemocytes, revealed the correlation between the virulence of Ma and the changes of enzymes activities in host induced by Ma, and clarified the critical period in the infection of Ma. So, these results should provide guidance for the construction of efficient biocontrol Ma strains.

Citing Articles

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References

Cerenius L, Soderhall K . The prophenoloxidase-activating system in invertebrates. Immunol Rev. 2004; 198:116-26. DOI: 10.1111/j.0105-2896.2004.00116.x. View

Gopalakrishnan S, Chen F, Thilagam H, Qiao K, Xu W, Wang K . Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides. Evid Based Complement Alternat Med. 2011; 2011:824962. PMC: 3118543. DOI: 10.1155/2011/824962. View

Lumjuan N, McCarroll L, Prapanthadara L, Hemingway J, Ranson H . Elevated activity of an Epsilon class glutathione transferase confers DDT resistance in the dengue vector, Aedes aegypti. Insect Biochem Mol Biol. 2005; 35(8):861-71. DOI: 10.1016/j.ibmb.2005.03.008. View

Serebrov V, Alekseev A, Glupov V . [Changes in the activity and pattern of hemolymph esterases in the larvae of wax moth Galleria mellonella L. (Lepidoptera, Pyralidae) during mycosis]. Izv Akad Nauk Ser Biol. 2005; (5):588-92. View

Weiser J, Matha V . The insecticidal activity of cyclosporines on mosquito larvae. J Invertebr Pathol. 1988; 51(1):92-3. DOI: 10.1016/0022-2011(88)90092-4. View

Enayati A, Ranson H, Hemingway J . Insect glutathione transferases and insecticide resistance. Insect Mol Biol. 2005; 14(1):3-8. DOI: 10.1111/j.1365-2583.2004.00529.x. View

Matsuda D, Namatame I, Tomoda H, Kobayashi S, Zocher R, Kleinkauf H . New beauveriolides produced by amino acid-supplemented fermentation of Beauveria sp. FO-6979. J Antibiot (Tokyo). 2004; 57(1):1-9. DOI: 10.7164/antibiotics.57.1. View

Mazmanian S, Round J, Kasper D . A microbial symbiosis factor prevents intestinal inflammatory disease. Nature. 2008; 453(7195):620-5. DOI: 10.1038/nature07008. View

Yassine H, Kamareddine L, Osta M . The mosquito melanization response is implicated in defense against the entomopathogenic fungus Beauveria bassiana. PLoS Pathog. 2012; 8(11):e1003029. PMC: 3499577. DOI: 10.1371/journal.ppat.1003029. View

10.

Barata C, Solayan A, Porte C . Role of B-esterases in assessing toxicity of organophosphorus (chlorpyrifos, malathion) and carbamate (carbofuran) pesticides to Daphnia magna. Aquat Toxicol. 2004; 66(2):125-39. DOI: 10.1016/j.aquatox.2003.07.004. View

11.

Ortelli F, Rossiter L, Vontas J, Ranson H, Hemingway J . Heterologous expression of four glutathione transferase genes genetically linked to a major insecticide-resistance locus from the malaria vector Anopheles gambiae. Biochem J. 2003; 373(Pt 3):957-63. PMC: 1223529. DOI: 10.1042/BJ20030169. View

12.

Wang C, St Leger R . A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses. Proc Natl Acad Sci U S A. 2006; 103(17):6647-52. PMC: 1458935. DOI: 10.1073/pnas.0601951103. View

13.

Lemaitre B, Hoffmann J . The host defense of Drosophila melanogaster. Annu Rev Immunol. 2007; 25:697-743. DOI: 10.1146/annurev.immunol.25.022106.141615. View

14.

Sandhu S, Sharma A, Beniwal V, Goel G, Batra P, Kumar A . Myco-biocontrol of insect pests: factors involved, mechanism, and regulation. J Pathog. 2012; 2012:126819. PMC: 3335529. DOI: 10.1155/2012/126819. View

15.

Hsu S, Lockwood J . Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol. 1975; 29(3):422-6. PMC: 186990. DOI: 10.1128/am.29.3.422-426.1975. View

16.

Contreras-Garduno J, Lanz-Mendoza H, Cordoba-Aguilar A . The expression of a sexually selected trait correlates with different immune defense components and survival in males of the American rubyspot. J Insect Physiol. 2007; 53(6):612-21. DOI: 10.1016/j.jinsphys.2007.03.003. View

17.

Chaurasia A, Lone Y, Wani O, Gupta U . Effect of certain entomopathogenic fungi on oxidative stress and mortality of Periplaneta americana. Pestic Biochem Physiol. 2016; 127:28-37. DOI: 10.1016/j.pestbp.2015.09.002. View

18.

TERRIERE L . Induction of detoxication enzymes in insects. Annu Rev Entomol. 1984; 29:71-88. DOI: 10.1146/annurev.en.29.010184.000443. View

19.

Toutant J . Insect acetylcholinesterase: catalytic properties, tissue distribution and molecular forms. Prog Neurobiol. 1989; 32(5):423-46. DOI: 10.1016/0301-0082(89)90031-2. View

20.

Gottar M, Gobert V, Matskevich A, Reichhart J, Wang C, Butt T . Dual detection of fungal infections in Drosophila via recognition of glucans and sensing of virulence factors. Cell. 2006; 127(7):1425-37. PMC: 1865096. DOI: 10.1016/j.cell.2006.10.046. View