Rapid Classification and Identification of Microcystis Aeruginosa Strains Using MALDI-TOF MS and Polygenetic Analysis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 May 27

PMID 27227555

Citations 9

Authors

Li-Wei Sun

Wen-Jing Jiang

Hiroaki Sato

Masanobu Kawachi

Xi-Wu Lu

Affiliations

Soon will be listed here.

Abstract

Matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was used to establish a rapid, simple, and accurate method to differentiate among strains of Microcystis aeruginosa, one of the most prevalent types of bloom-forming cyanobacteria. M. aeruginosa NIES-843, for which a complete genome has been sequenced, was used to characterize ribosomal proteins as biomarkers and to optimize conditions for observing ribosomal proteins as major peaks in a given mass spectrum. Thirty-one of 52 ribosomal subunit proteins were detected and identified along the mass spectrum. Fifty-five strains of M. aeruginosa from different habitats were analyzed using MALDI-TOF MS; among these samples, different ribosomal protein types were observed. A polygenetic analysis was performed using an unweighted pair-group method with arithmetic means and different ribosomal protein types to classify the strains into five major clades. Two clades primarily contained toxic strains, and the other three clades contained exclusively non-toxic strains. This is the first study to differentiate cyanobacterial strains using MALDI-TOF MS.

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