Molecular Diversity of Seed-borne Fusarium Species Associated with Maize in India

Overview

Journal Curr Genomics

Publisher Bentham Science Publishers

Date 2016 May 27

PMID 27226769

Citations 7

Authors

Mohammed Aiyaz

Shetty Thimmappa Divakara

Venkataramana Mudili

Geromy George Moore

Vijai Kumar Gupta

Tapani Yli-Mattila

Siddaiah Chandra Nayaka

Siddapura Ramachandrappa Niranjana

Affiliations

Soon will be listed here.

Abstract

A total of 106 maize seed samples were collected from different agro-climatic regions of India. Sixty-two Fusarium isolates were recovered, 90% of which were identified as Fusarium verticillioides based on morphological and molecular characters. Use of the tef-1α gene corrected/refined the morphological species identifications of 11 isolates, and confirmed those of the remaining isolates. Genetic diversity among the Fusarium isolates involved multilocus fingerprinting profiles by Inter Simple Sequence Repeats (ISSR) UPGMA and tef-1α gene phenetic analyses; for which, we observed no significant differences among the isolates based on geographic origin or fumonisin production; most of the subdivision related to species. Genotyping was performed on the F. verticillioides isolates, using 12 primer sets from the fumonisin pathway, to elucidate the molec-ular basis of fumonisin production or non-production. One fumonisin-negative isolate, UOMMF-16, was unable to amplify nine of the 12 fumonisin cluster genes tested. We also used the CD-ELISA method to confirm fumonisin production for our 62 Fusarium isolates. Only 15 isolates were found to be fumonisin-negative. Interestingly, genotypic characterization re-vealed six isolates with various gene deletion patterns that also tested positive for the production of fumonisins via CD-ELISA. Our findings confirm the importance of molecular studies for species delimitation, and for observing genetic and phenotypic diversity, among the Fusaria.

Citing Articles

Molecular identification and pathogenicity of endophytic fungi from corn ears.

Terna P, Mohamed Nor N, Azuddin N, Zakaria L Sci Rep. 2024; 14(1):17146.

PMID: 39060380 PMC: 11282103. DOI: 10.1038/s41598-024-68428-1.

and and Their Main Mycotoxins: Global Distribution and Scenarios of Interactions in Maize.

Chen X, Abdallah M, Landschoot S, Audenaert K, De Saeger S, Chen X Toxins (Basel). 2023; 15(9).

PMID: 37756003 PMC: 10534665. DOI: 10.3390/toxins15090577.

Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India.

Navale V, Sawant A, Vamkudoth K Genet Mol Biol. 2023; 46(1):e20220073.

PMID: 37036389 PMC: 10084715. DOI: 10.1590/1678-4685-GMB-2022-0073.

Assembly, Annotation, and Comparative Whole Genome Sequence of Isolated from Stored Maize Grains.

Navale V, Sawant A, Gowda V, Vamkudoth K Pathogens. 2022; 11(7).

PMID: 35890054 PMC: 9320718. DOI: 10.3390/pathogens11070810.

Multigene Phylogeny, Beauvericin Production and Bioactive Potential of Strains Isolated in India.

Rana S, Singh S, Dufosse L J Fungi (Basel). 2022; 8(7).

PMID: 35887419 PMC: 9320867. DOI: 10.3390/jof8070662.

References

Streit E, Schatzmayr G, Tassis P, Tzika E, Marin D, Taranu I . Current situation of mycotoxin contamination and co-occurrence in animal feed--focus on Europe. Toxins (Basel). 2012; 4(10):788-809. PMC: 3496989. DOI: 10.3390/toxins4100788. View

Thiel P, Marasas W, Sydenham E, Shephard G, Gelderblom W, Nieuwenhuis J . Survey of fumonisin production by Fusarium species. Appl Environ Microbiol. 1991; 57(4):1089-93. PMC: 182850. DOI: 10.1128/aem.57.4.1089-1093.1991. View

Vitale S, Santori A, Wajnberg E, Castagnone-Sereno P, Luongo L, Belisario A . Morphological and molecular analysis of Fusarium lateritium, the cause of gray necrosis of hazelnut fruit in Italy. Phytopathology. 2011; 101(6):679-86. DOI: 10.1094/PHYTO-04-10-0120. View

Paranagama P, Wijeratne E, Gunatilaka A . Uncovering biosynthetic potential of plant-associated fungi: effect of culture conditions on metabolite production by Paraphaeosphaeria quadriseptata and Chaetomium chiversii. J Nat Prod. 2007; 70(12):1939-45. DOI: 10.1021/np070504b. View

Mogensen J, Nielsen K, Samson R, Frisvad J, Thrane U . Effect of temperature and water activity on the production of fumonisins by Aspergillus niger and different Fusarium species. BMC Microbiol. 2010; 9:281. PMC: 2811119. DOI: 10.1186/1471-2180-9-281. View

Yu F, Zhu X, Du L . Developing a genetic system for functional manipulations of FUM1, a polyketide synthase gene for the biosynthesis of fumonisins in Fusarium verticillioides. FEMS Microbiol Lett. 2005; 248(2):257-64. DOI: 10.1016/j.femsle.2005.05.053. View

Miller J . Factors that affect the occurrence of fumonisin. Environ Health Perspect. 2001; 109 Suppl 2:321-4. PMC: 1240682. DOI: 10.1289/ehp.01109s2321. View

Ramana M, Balakrishna K, Murali H, Batra H . Multiplex PCR-based strategy to detect contamination with mycotoxigenic Fusarium species in rice and fingermillet collected from southern India. J Sci Food Agric. 2011; 91(9):1666-73. DOI: 10.1002/jsfa.4365. View

Venkataramana M, Navya K, Chandranayaka S, Priyanka S, Murali H, Batra H . Development and validation of an immunochromatographic assay for rapid detection of fumonisin B1 from cereal samples. J Food Sci Technol. 2014; 51(9):1920-8. PMC: 4152549. DOI: 10.1007/s13197-013-1254-x. View

10.

Summerell B, Salleh B, Leslie J . A Utilitarian Approach to Fusarium Identification. Plant Dis. 2019; 87(2):117-128. DOI: 10.1094/PDIS.2003.87.2.117. View

11.

Nelson P, Desjardins A, Plattner R . Fumonisins, mycotoxins produced by fusarium species: biology, chemistry, and significance. Annu Rev Phytopathol. 1993; 31:233-52. DOI: 10.1146/annurev.py.31.090193.001313. View

12.

Proctor R, Plattner R, Desjardins A, Busman M, Butchko R . Fumonisin production in the maize pathogen Fusarium verticillioides: genetic basis of naturally occurring chemical variation. J Agric Food Chem. 2006; 54(6):2424-30. DOI: 10.1021/jf0527706. View

13.

Rheeder J, Marasas W, Vismer H . Production of fumonisin analogs by Fusarium species. Appl Environ Microbiol. 2002; 68(5):2101-5. PMC: 127586. DOI: 10.1128/AEM.68.5.2101-2105.2002. View

14.

Maheshwar P, Moharram S, Janardhana G . Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR). Braz J Microbiol. 2013; 40(1):134-8. PMC: 3768486. DOI: 10.1590/S1517-838220090001000023. View

15.

Sanchez-Rangel D, Sanjuan-Badillo A, Plasencia J . Fumonisin production by Fusarium verticillioides strains isolated from maize in Mexico and development of a polymerase chain reaction to detect potential toxigenic strains in grains. J Agric Food Chem. 2005; 53(22):8565-71. DOI: 10.1021/jf0514827. View

16.

Kimura M . A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980; 16(2):111-20. DOI: 10.1007/BF01731581. View

17.

Dissanayake M, Tanaka S, Ito S . Fumonisin B(1) production by Fusarium proliferatum strains isolated from Allium fistulosum plants and seeds in Japan. Lett Appl Microbiol. 2009; 48(5):598-604. DOI: 10.1111/j.1472-765X.2009.02576.x. View

18.

Thangavelu R, Kumar K, Devi P, Mustaffa M . Genetic diversity of Fusarium oxysporum f.sp. cubense isolates (Foc) of India by inter simple sequence repeats (ISSR) analysis. Mol Biotechnol. 2011; 51(3):203-11. DOI: 10.1007/s12033-011-9457-8. View

19.

Chandra N, Wulff E, Udayashankar A, Nandini B, Niranjana S, Mortensen C . Prospects of molecular markers in Fusarium species diversity. Appl Microbiol Biotechnol. 2011; 90(5):1625-39. DOI: 10.1007/s00253-011-3209-3. View

20.

Niessen L . PCR-based diagnosis and quantification of mycotoxin-producing fungi. Adv Food Nutr Res. 2008; 54:81-138. DOI: 10.1016/S1043-4526(07)00003-4. View