Effects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and Its Definite Dose Reduction Factor

Overview

Journal Iran J Med Sci

Publisher Shiraz University of Medical Sciences

Specialty General Medicine

Date 2016 May 25

PMID 27217601

Citations 5

Authors

Saba Nadi

Ali Shabestani Monfared

Hossein Mozdarani

Aziz Mahmodzade

Mahdi Pouramir

Affiliations

Soon will be listed here.

Abstract

Background: Interactions of free radicals from ionizing radiation with DNA can induce DNA damage and lead to mutagenesis and carsinogenesis. With respect to radiation damage to human, it is important to protect humans from side effects induced by ionizing radiation. In the present study, the effects of arbutin were investigated by using the micronucleus test for anti-clastogenic activity, to calculate the ratio of polychromatic erythrocyte to polychromatic erythrocyte plus normochromatic erythrocyte (PCE/PCE+NCE) in order to show cell proliferation activity.

Methods: Arbutin (50, 100, and 200 mg/kg) was intraperitoneally (ip)administered to NMRI mice two hours before gamma radiation at 2 and 4 gray (Gy). The frequency of micronuclei in 1000 PCEs (MnPCEs) and the ratio of PCE/PCE+NCE were calculated for each sample. Data were statistically evaluated using one-way ANOVA, Tukey HSD test, and t-test.

Results: The findings indicated that gamma radiation at 2 and 4 Gy extremely increased the frequencies of MnPCE (P<0.001) while reducing PCE/PCE+NCE (P<0.001) compared to the control group. All three doses of arbutin before irradiation significantly reduced the frequencies of MnPCEs and increased the ratio of PCE/PCE+NCE in mice bone marrow compared to the non-drug-treated irradiated control (P<0.001). All three doses of arbutin had no toxicity effect on bone marrow cells. The calculated dose reduction factor (DRF) showed DRF=1.93 for 2Gy and DRF=2.22 for 4 Gy.

Conclusion: Our results demonstrated that arbutin gives significant protection to rat bone against the clastogenic and cytotoxic effects of gamma irradiation.

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References

Hosseinimehr S, Tavakoli H, Pourheidari G, Sobhani A, Shafiee A . Radioprotective effects of citrus extract against gamma-irradiation in mouse bone marrow cells. J Radiat Res. 2003; 44(3):237-41. DOI: 10.1269/jrr.44.237. View

Schmid W . The micronucleus test. Mutat Res. 1975; 31(1):9-15. DOI: 10.1016/0165-1161(75)90058-8. View

Hosseinimehr S, Nemati A . Radioprotective effects of hesperidin against gamma irradiation in mouse bone marrow cells. Br J Radiol. 2006; 79(941):415-8. DOI: 10.1259/bjr/40692384. View

Mozdarani H, Gharbali A . Radioprotective effects of cimetidine in mouse bone marrow cells exposed to gamma-rays as assayed by the micronucleus test. Int J Radiat Biol. 1993; 64(2):189-94. DOI: 10.1080/09553009314551291. View

Takebayashi J, Ishii R, Chen J, Matsumoto T, Ishimi Y, Tai A . Reassessment of antioxidant activity of arbutin: multifaceted evaluation using five antioxidant assay systems. Free Radic Res. 2010; 44(4):473-8. DOI: 10.3109/10715761003610760. View

Wallner M, Blassnigg S, Marisch K, Pappenheim M, Mullner E, Molzer C . Effects of unconjugated bilirubin on chromosomal damage in individuals with Gilbert's syndrome measured with the micronucleus cytome assay. Mutagenesis. 2012; 27(6):731-5. DOI: 10.1093/mutage/ges039. View

Kang M, Ha H, Kim H, Lee D, Kong M, Ahn Y . Role of metabolism by intestinal bacteria in arbutin-induced toxicity in vitro. Arch Pharm Res. 2011; 34(4):687-93. DOI: 10.1007/s12272-011-0420-9. View

McNamee J, Flegal F, Boulay Greene H, Marro L, Wilkins R . Validation of the cytokinesis-block micronucleus (CBMN) assay for use as a triage biological dosimetry tool. Radiat Prot Dosimetry. 2009; 135(4):232-42. DOI: 10.1093/rpd/ncp119. View

Cardoso R, Peitl Jr P, Sakamoto-Hojo E . Evaluation of chromosomal aberrations, micronuclei, and sister chromatid exchanges in hospital workers chronically exposed to ionizing radiation. Teratog Carcinog Mutagen. 2001; 21(6):431-9. DOI: 10.1002/tcm.1030. View

10.

Alsbeih G, El-Sebaie M, Al-Harbi N, Al-Buhairi M, Al-Hadyan K, Al-Rajhi N . Radiosensitivity of human fibroblasts is associated with amino acid substitution variants in susceptible genes and correlates with the number of risk alleles. Int J Radiat Oncol Biol Phys. 2007; 68(1):229-35. DOI: 10.1016/j.ijrobp.2006.12.050. View

11.

Shahaboddin M, Pouramir M, Moghadamnia A, Parsian H, Lakzaei M, Mir H . Pyrus biossieriana Buhse leaf extract: An antioxidant, antihyperglycaemic and antihyperlipidemic agent. Food Chem. 2014; 126(4):1730-3. DOI: 10.1016/j.foodchem.2010.12.069. View

12.

Shokrzadeh M, Naghshvar F, Ahmadi A, Chabra A, Jeivad F . The potential ameliorative effects of melatonin against cyclophosphamide-induced DNA damage in murine bone marrow cells. Eur Rev Med Pharmacol Sci. 2014; 18(5):605-11. View

13.

Maffei F, Angelini S, Cantelli Forti G, Lodi V, Violante F, Mattioli S . Micronuclei frequencies in hospital workers occupationally exposed to low levels of ionizing radiation: influence of smoking status and other factors. Mutagenesis. 2002; 17(5):405-9. DOI: 10.1093/mutage/17.5.405. View

14.

Fenech M . Cytokinesis-block micronucleus cytome assay. Nat Protoc. 2007; 2(5):1084-104. DOI: 10.1038/nprot.2007.77. View

15.

Mozdarani H . Radioprotective properties of histamine H2 receptor antagonists: present and future prospects. J Radiat Res. 2003; 44(2):145-9. DOI: 10.1269/jrr.44.145. View

16.

Hori I, Nihei K, Kubo I . Structural criteria for depigmenting mechanism of arbutin. Phytother Res. 2004; 18(6):475-9. DOI: 10.1002/ptr.1456. View

17.

Thierens H, Vral A, Morthier R, Aousalah B, de Ridder L . Cytogenetic monitoring of hospital workers occupationally exposed to ionizing radiation using the micronucleus centromere assay. Mutagenesis. 2000; 15(3):245-9. DOI: 10.1093/mutage/15.3.245. View

18.

Cheng S, Huang Liu R, Sheu J, Chen S, Sinchaikul S, Tsay G . Toxicogenomics of A375 human malignant melanoma cells treated with arbutin. J Biomed Sci. 2006; 14(1):87-105. DOI: 10.1007/s11373-006-9130-6. View

19.

Lenarczyk M, Goddu S, Rao D, Howell R . Biologic dosimetry of bone marrow: induction of micronuclei in reticulocytes after exposure to 32P and 90Y. J Nucl Med. 2001; 42(1):162-9. View

20.

Watanabe N, Kanegane H, Kinuya S, Shuke N, Yokoyama K, Kato H . The radiotoxicity of 131I therapy of thyroid cancer: assessment by micronucleus assay of B lymphocytes. J Nucl Med. 2004; 45(4):608-11. View