Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2016 May 22

PMID 27207283

Citations 5

Authors

Michael Maes

Joost Willebrords

Sara Crespo Yanguas

Bruno Cogliati

Mathieu Vinken

Affiliations

Soon will be listed here.

Abstract

Although connexin production is mainly regulated at the protein level, altered connexin gene expression has been identified as the underlying mechanism of several pathologies. When studying the latter, appropriate methods to quantify connexin RNA levels are required. The present chapter describes a well-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction, and data analysis.

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