Genetic Dissection of the Control of Normal Differentiation in Myeloid Leukemic Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Dec 1

PMID 271980

Citations 23

Authors

J Lotem

L Sachs

Affiliations

Soon will be listed here.

Abstract

Normal myeloid precursors and MGI(+)D(+) myeloid leukemic cells can be induced to differentiate to mature cells by the normal protein inducer MGI. The sequence of differentiation is the induction of C3 and Fc rosettes, C3 and Fc immune phagocytosis (IP), synthesis and secretion of lysozyme, and formation of mature macrophages and granulocytes. Mutant clones of myeloid leukemic cells have been isolated with differences in the time of induction of C3 and Fc rosettes and C3 and Fc IP, in which lysozyme was induced without going through the stage of Fc or C3 IP, and with differences in inducibility by MGI to mature macrophages or granulocytes. Only one out of five MGI(-)D(-) clones gave rise to MGI(+)D(+) mutants. The ability to obtain mutants from this clone was associated with its special chromosome constitution, and these mutants showed a change in their ability for cap formation by concanavalin A. The steroid inducer dexamethasone can induce in MGI(+)D(+) clones differentiation to macrophages but not to granulocytes. Differentiation by steroid inducer in different clones occurred either with or without induction of Fc rosettes and Fc IP, and induction of C3 rosettes was not always associated with induction of C3 IP. The use of mutants that differ in their competence to be induced by MGI or steroid inducer has shown that there are separate controls for the induction of C3 and Fc rosettes, C3 and Fc IP, lysozyme, macrophages, and granulocytes.

Citing Articles

NQO1 stabilizes p53 through a distinct pathway.

Asher G, Lotem J, Kama R, Sachs L, Shaul Y Proc Natl Acad Sci U S A. 2002; 99(5):3099-104.

PMID: 11867746 PMC: 122479. DOI: 10.1073/pnas.052706799.

Cytokine suppression of protease activation in wild-type p53-dependent and p53-independent apoptosis.

Lotem J, Sachs L Proc Natl Acad Sci U S A. 1997; 94(17):9349-53.

PMID: 9256485 PMC: 23189. DOI: 10.1073/pnas.94.17.9349.

Differential suppression by protease inhibitors and cytokines of apoptosis induced by wild-type p53 and cytotoxic agents.

Lotem J, Sachs L Proc Natl Acad Sci U S A. 1996; 93(22):12507-12.

PMID: 8901612 PMC: 38022. DOI: 10.1073/pnas.93.22.12507.

Cellular oxidative stress and the control of apoptosis by wild-type p53, cytotoxic compounds, and cytokines.

Lotem J, Groner Y, Sachs L Proc Natl Acad Sci U S A. 1996; 93(17):9166-71.

PMID: 8799172 PMC: 38613. DOI: 10.1073/pnas.93.17.9166.

The control of hematopoiesis and leukemia: from basic biology to the clinic.

Sachs L Proc Natl Acad Sci U S A. 1996; 93(10):4742-9.

PMID: 8643473 PMC: 39349. DOI: 10.1073/pnas.93.10.4742.

References

Ginsburg H, Sachs L . FORMATION OF PURE SUSPENSIONS OF MAST CELLS IN TISSUE CULTURE BY DIFFERENTIATION OF LYMPHOID CELLS FROM THE MOUSE THYMUS. J Natl Cancer Inst. 1963; 31:1-39. View

Nudel U, Salmon J, Terada M, Bank A, Rifkind R, Marks P . Differential effects of chemical inducers on expression of beta globin genes in murine erythroleukemia cells. Proc Natl Acad Sci U S A. 1977; 74(3):1100-4. PMC: 430606. DOI: 10.1073/pnas.74.3.1100. View

Lotem J, Sachs L . Control of normal differentiation of myeloid leukemic cells. XII. Isolation of normal myeloid colony-forming cells from bone marrow and the sequence of differentiation to mature granulocytes in normal and D+ myeloid leukemic cells. J Cell Physiol. 1977; 92(1):97-108. DOI: 10.1002/jcp.1040920112. View

Azumi J, Sachs L . Chromosome mapping of the genes that control differentiation and malignancy in myeloid leukemic cells. Proc Natl Acad Sci U S A. 1977; 74(1):253-7. PMC: 393237. DOI: 10.1073/pnas.74.1.253. View

Krystosek A, Sachs L . Control of lysozyme induction in the differentiation of myeloid leukemic cells. Cell. 1976; 9(4 PT 2):675-84. DOI: 10.1016/0092-8674(76)90131-8. View

Fibach E, Sachs L . Control of normal differentiation of myeloid leukemic cells. XI. Induction of a specific requirement for cell viability and growth during the differentiation of myeloid leukemic cells. J Cell Physiol. 1976; 89(2):259-66. DOI: 10.1002/jcp.1040890209. View

KLUGE N, Gaedicke G, Steinheider G, Dube S, Ostertag W . Globin synthesis in Friend-erythroleukemia mouse cells in protein- and lipid-free medium. Effects of dimethyl-sulfoxide, iron and erythropoietin. Exp Cell Res. 1974; 88(2):257-62. DOI: 10.1016/0014-4827(74)90239-0. View

Bradley T, Metcalf D . The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci. 1966; 44(3):287-99. DOI: 10.1038/icb.1966.28. View

Ichikawa Y . Differentiation of a cell line of myeloid leukemia. J Cell Physiol. 1969; 74(3):223-34. DOI: 10.1002/jcp.1040740303. View

10.

Austin P, McCulloch E, Till J . Characterization of the factor in L-cell conditioned medium capable of stimulating colony formation by mouse marrow cells in culture. J Cell Physiol. 1971; 77(2):121-34. DOI: 10.1002/jcp.1040770202. View

11.

Bianco C, Patrick R, NUSSENZWEIG V . A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970; 132(4):702-20. PMC: 2138865. DOI: 10.1084/jem.132.4.702. View

12.

Ohta Y, Tanaka M, Terada M, MILLER O, Bank A, Marks P . Erythroid cell differentiation: murine erythroleukemia cell variant with unique pattern of induction by polar compounds. Proc Natl Acad Sci U S A. 1976; 73(4):1232-6. PMC: 430236. DOI: 10.1073/pnas.73.4.1232. View

13.

Sachs L . Regulation of membrane changes, differentiation, and malignancy in carcinogenesis. Harvey Lect. 1974; 68:1-35. View

14.

Cuatrecasas P . Membrane receptors. Annu Rev Biochem. 1974; 43(0):169-214. DOI: 10.1146/annurev.bi.43.070174.001125. View

15.

Landau T, Sachs L . Characterization of the inducer required for the development of macrophage and granulocyte colonies. Proc Natl Acad Sci U S A. 1971; 68(10):2540-4. PMC: 389464. DOI: 10.1073/pnas.68.10.2540. View

16.

Edelman G, YAHARA I, Wang J . Receptor mobility and receptor-cytoplasmic interactions in lymphocytes. Proc Natl Acad Sci U S A. 1973; 70(5):1442-6. PMC: 433515. DOI: 10.1073/pnas.70.5.1442. View

17.

Fibach E, Hayashi M, Sachs L . Control of normal differentiation of myeloid leukemic cells to macrophages and granulocytes. Proc Natl Acad Sci U S A. 1973; 70(2):343-6. PMC: 433254. DOI: 10.1073/pnas.70.2.343. View

18.

Lotem J, Sachs L . Different blocks in the differentiation of myeloid leukemic cells. Proc Natl Acad Sci U S A. 1974; 71(9):3507-11. PMC: 433803. DOI: 10.1073/pnas.71.9.3507. View

19.

Lotem J, Sachs L . Control of normal differentiation of myeloid leukemic cells. VI. Inhibition of cell multiplication and the formation of macrophages. J Cell Physiol. 1975; 85(3):587-94. DOI: 10.1002/jcp.1040850310. View

20.

Gordon S, Todd J, COHN Z . In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. J Exp Med. 1974; 139(5):1228-48. PMC: 2139654. DOI: 10.1084/jem.139.5.1228. View