The Influence of Dexamethasone and the Role of Some Antioxidant Vitamins in the Pathogenesis of Experimental Bronchial Asthma

Overview

Journal J Exp Pharmacol

Publisher Dove Medical Press

Date 2016 May 18

PMID 27186095

Citations 2

Authors

H A Nounou

M M Deif

M Arafah

Affiliations

Soon will be listed here.

Abstract

Background: Bronchial asthma is a disease characterized by paroxysmal and reversible obstruction of the airways. The imbalance between the oxidant and antioxidant system that is called oxidative stress is critical in asthma pathogenesis. It is likely, therefore, that antioxidants may be effective in the treatment of asthma. Systemic treatment with glucocorticoids has been reported to inhibit smooth muscle hypercontraction which may account partially for their beneficial effects in the treatment of asthma.

Objective: The present study was conducted in order to study the effect of dexamethasone and some antioxidant vitamins on interleukin-4 (IL-4), immunoglobulin E (IgE) and heat shock protein 70 (Hsp70) in bronchial asthma in rats, and to recognize their possible beneficial role.

Method: The study was conducted on 60 adult male albino rats randomly divided into 4 groups (15 for each group): including normal control group (group A); asthma model group where rats were sensitized by ovalbumin and challenged with antigen aerosol producing bronchial asthma (group B); asthma model group treated with antioxidant vitamins (vitamin E and vitamin C) (group C); asthma model group treated with dexamethasone (group D). Blood and lung samples were collected from all groups.

Results And Conclusion: Our results revealed a significant decrease of serum reduced glutathione (GSH) levels among groups B, C and D as compared to group A, while there was a significant increase in group C and D as compared to group B. Antioxidant and dexamethasone treatment resulted in a significant decrease of serum IL-4, malondialdehyde (MDA), and serum IgE levels in group C and D as compared to group B. Antioxidant treatment resulted in a significant decrease of serum Hsp70 level as compared to group B, while dexamethasone treatment resulted in a significant increase of serum Hsp70 level as compared to group B. This study suggests that it is likely that a combination of antioxidant vitamins may be effective in the treatment of asthma, considering their reported effects on lowering MDA, IL-4, and IgE levels, and the similar beneficial effects of dexamethasone in addition to increasing the expression of Hsp70 in the studied model of bronchial asthma.

Citing Articles

The Role of Vitamins in the Pathogenesis of Asthma.

Zajac D, Wojciechowski P Int J Mol Sci. 2023; 24(10).

PMID: 37239921 PMC: 10218465. DOI: 10.3390/ijms24108574.

Protective effects of ascorbic acid and calcitriol combination on airway remodelling in ovalbumin-induced chronic asthma.

Kianian F, Karimian S, Kadkhodaee M, Takzaree N, Seifi B, Sadeghipour H Pharm Biol. 2020; 58(1):107-115.

PMID: 31942829 PMC: 7006747. DOI: 10.1080/13880209.2019.1710218.

References

. Recommended methods for an additional red cell enzyme (pyrimidine 5'-nucleotidase) assay and the determination of red cell adenosine-5'-triphosphate, 2,3-diphosphoglycerate and reduced glutathione. International Committee for Standardization in.... Clin Lab Haematol. 1989; 11(2):131-8. DOI: 10.1111/j.1365-2257.1989.tb00195.x. View

Horvath G, Wanner A . Inhaled corticosteroids: effects on the airway vasculature in bronchial asthma. Eur Respir J. 2006; 27(1):172-87. DOI: 10.1183/09031936.06.00048605. View

Khassaf M, McArdle A, ESANU C, Vasilaki A, McArdle F, Griffiths R . Effect of vitamin C supplements on antioxidant defence and stress proteins in human lymphocytes and skeletal muscle. J Physiol. 2003; 549(Pt 2):645-52. PMC: 2342961. DOI: 10.1113/jphysiol.2003.040303. View

Murphy K, Reiner S . The lineage decisions of helper T cells. Nat Rev Immunol. 2002; 2(12):933-44. DOI: 10.1038/nri954. View

Pearson P, Lewis S, Britton J, Fogarty A . Vitamin E supplements in asthma: a parallel group randomised placebo controlled trial. Thorax. 2004; 59(8):652-6. PMC: 1747112. DOI: 10.1136/thx.2004.022616. View

Singh-Jasuja H, Hilf N, Schild H . The role of heat shock proteins and their receptors in the activation of the immune system. Biol Chem. 2001; 382(4):629-36. DOI: 10.1515/BC.2001.074. View

Xiao C, Wu T, Ren A, Pan Q, Chen S, Wu F . Basal and inducible levels of Hsp70 in patients with acute heat illness induced during training. Cell Stress Chaperones. 2003; 8(1):86-92. PMC: 514858. DOI: 10.1379/1466-1268(2003)8<86:bailoh>2.0.co;2. View

Keles M, Taysi S, Sen N, Aksoy H, Akcay F . Effect of corticosteroid therapy on serum and CSF malondialdehyde and antioxidant proteins in multiple sclerosis. Can J Neurol Sci. 2001; 28(2):141-3. DOI: 10.1017/s0317167100052823. View

Konishi T, Nomoto M, Shimizu K, Abe T, Itoh H . Dominant role of the second heat shock element in expression of the HSP70-1 gene in rat liver after whole body hyperthermia. J Biochem. 1995; 118(5):1021-9. DOI: 10.1093/jb/118.5.1021. View

10.

Hamelmann E, Tadeda K, Oshiba A, Gelfand E . Role of IgE in the development of allergic airway inflammation and airway hyperresponsiveness--a murine model. Allergy. 1999; 54(4):297-305. DOI: 10.1034/j.1398-9995.1999.00085.x. View

11.

Walther F, Mehta E, Polk D, Jobe A, Ikegami M . Higher lung antioxidant enzyme activity persists after single dose of corticosteroids in preterm lambs. Am J Physiol. 1996; 271(2 Pt 1):L187-91. DOI: 10.1152/ajplung.1996.271.2.L187. View

12.

Devereux G, Seaton A . Diet as a risk factor for atopy and asthma. J Allergy Clin Immunol. 2005; 115(6):1109-17. DOI: 10.1016/j.jaci.2004.12.1139. View

13.

Miller M, Cho J, McElwain K, McElwain S, Shim J, Manni M . Corticosteroids prevent myofibroblast accumulation and airway remodeling in mice. Am J Physiol Lung Cell Mol Physiol. 2005; 290(1):L162-9. DOI: 10.1152/ajplung.00252.2005. View

14.

Gray C, Amrani M, Yacoub M . Heat stress proteins and myocardial protection: experimental model or potential clinical tool?. Int J Biochem Cell Biol. 1999; 31(5):559-73. DOI: 10.1016/s1357-2725(99)00004-7. View

15.

Kirkham P, Rahman I . Oxidative stress in asthma and COPD: antioxidants as a therapeutic strategy. Pharmacol Ther. 2006; 111(2):476-94. DOI: 10.1016/j.pharmthera.2005.10.015. View

16.

Shang F, Lu M, Dudek E, Reddan J, Taylor A . Vitamin C and vitamin E restore the resistance of GSH-depleted lens cells to H2O2. Free Radic Biol Med. 2003; 34(5):521-30. DOI: 10.1016/s0891-5849(02)01304-7. View

17.

Albers R, Bol M, Bleumink R, Willems A, Pieters R . Effects of supplementation with vitamins A, C, and E, selenium, and zinc on immune function in a murine sensitization model. Nutrition. 2003; 19(11-12):940-6. DOI: 10.1016/s0899-9007(03)00178-3. View

18.

Rahman I . The role of oxidative stress in the pathogenesis of COPD: implications for therapy. Treat Respir Med. 2005; 4(3):175-200. DOI: 10.2165/00151829-200504030-00003. View

19.

Ravagnan L, Gurbuxani S, Susin S, Maisse C, Daugas E, Zamzami N . Heat-shock protein 70 antagonizes apoptosis-inducing factor. Nat Cell Biol. 2001; 3(9):839-43. DOI: 10.1038/ncb0901-839. View

20.

Suresh R, Shally A, Mahdi A, Patel D, Singh V, Rita M . Assessment of association of exposure to polycyclic aromatic hydrocarbons with bronchial asthma and oxidative stress in children: A case control study. Indian J Occup Environ Med. 2010; 13(1):33-7. PMC: 2822167. DOI: 10.4103/0019-5278.50722. View