The Drosophila Dicer-1 Partner Loquacious Enhances MiRNA Processing from Hairpins with Unstable Structures at the Dicing Site

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2016 May 18

PMID 27184838

Citations 12

Authors

Mandy Yu Theng Lim

Alvin Wei Tian Ng

Yuting Chou

Teck Por Lim

Amanda Simcox

Greg Tucker-Kellogg

Katsutomo Okamura

Affiliations

Soon will be listed here.

Abstract

In Drosophila, Dicer-1 binds Loquacious-PB (Loqs-PB) as its major co-factor. Previous analyses indicated that loqs mutants only partially impede miRNA processing, but the activity of minor isoforms or maternally deposited Loqs was not eliminated in these studies. We addressed this by generating a cell line from loqs-null embryos and found that only ∼40% of miRNAs showed clear Loqs dependence. Genome-wide comparison of the hairpin structure and Loqs dependence suggested that Loqs substrates are influenced by base-pairing status at the dicing site. Artificial alteration of base-pairing stability at this position in model miRNA hairpins resulted in predicted changes in Loqs dependence, providing evidence for this hypothesis. Finally, we found that evolutionarily young miRNA genes tended to be Loqs dependent. We propose that Loqs may have roles in assisting the de novo emergence of miRNA genes by facilitating dicing of suboptimal hairpin substrates.

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