A Simultaneous Two-colour Detection Method of Human IgG- and IgE-reactive Proteins from Lactic Acid Bacteria

Overview

Journal J Microbiol Methods

Specialty Microbiology

Date 2016 May 18

PMID 27184086

Citations 6

Authors

Lidia Hanna Markiewicz

Agata Szymkiewicz

Anna Szyc

Barbara Wroblewska

Affiliations

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Abstract

Whole cell extracts of two Lactobacillus strains were tested with primary antibodies from two pooled sera from allergic patients. Fluorescently labelled anti-human IgG and anti-human IgE secondary antibodies applied in Western blotting, together with an appropriate image acquisition protocol facilitated imagining bacterial proteins that reacted with human IgG and IgE.

Citing Articles

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Zlotkowska D, Markiewicz L, Ogrodowczyk A, Wroblewska B, Wasilewska E Nutrients. 2024; 16(20).

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Study protocol: The role of milk matrix lipids in programming the immunoreactivity of proteins derived from lactic acid bacteria.

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