Free-living Amoebae and Their Associated Bacteria in Austrian Cooling Towers: a 1-year Routine Screening

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2016 May 15

PMID 27177720

Citations 18

Authors

Ute Scheikl

Han-Fei Tsao

Matthias Horn

Alexander Indra

Julia Walochnik

Affiliations

Soon will be listed here.

Abstract

Free-living amoebae (FLA) are widely spread in the environment and known to cause rare but often serious infections. Besides this, FLA may serve as vehicles for bacterial pathogens. In particular, Legionella pneumophila is known to replicate within FLA thereby also gaining enhanced infectivity. Cooling towers have been the source of outbreaks of Legionnaires' disease in the past and are thus usually screened for legionellae on a routine basis, not considering, however, FLA and their vehicle function. The aim of this study was to incorporate a screening system for host amoebae into a Legionella routine screening. A new real-time PCR-based screening system for various groups of FLA was established. Three cooling towers were screened every 2 weeks over the period of 1 year for FLA and Legionella spp., by culture and molecular methods in parallel. Altogether, 83.3 % of the cooling tower samples were positive for FLA, Acanthamoeba being the dominating genus. Interestingly, 69.7 % of the cooling tower samples were not suitable for the standard Legionella screening due to their high organic burden. In the remaining samples, positivity for Legionella spp. was 25 % by culture, but overall positivity was 50 % by molecular methods. Several amoebal isolates revealed intracellular bacteria.

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References

La Scola B, Raoult D . Survival of Coxiella burnetii within free-living amoeba Acanthamoeba castellanii. Clin Microbiol Infect. 2001; 7(2):75-9. DOI: 10.1046/j.1469-0691.2001.00193.x. View

Wadowsky R, Wolford R, McNamara A, Yee R . Effect of temperature, pH, and oxygen level on the multiplication of naturally occurring Legionella pneumophila in potable water. Appl Environ Microbiol. 1985; 49(5):1197-205. PMC: 238529. DOI: 10.1128/aem.49.5.1197-1205.1985. View

Garcia M, Jones S, Pelaz C, Millar R, Abu Kwaik Y . Acanthamoeba polyphaga resuscitates viable non-culturable Legionella pneumophila after disinfection. Environ Microbiol. 2007; 9(5):1267-77. DOI: 10.1111/j.1462-2920.2007.01245.x. View

Corsaro D, Michel R, Walochnik J, Muller K, Greub G . Saccamoeba lacustris, sp. nov. (Amoebozoa: Lobosea: Hartmannellidae), a new lobose amoeba, parasitized by the novel chlamydia 'Candidatus Metachlamydia lacustris' (Chlamydiae: Parachlamydiaceae). Eur J Protistol. 2010; 46(2):86-95. DOI: 10.1016/j.ejop.2009.11.002. View

Hwang M, Katayama H, Ohgaki S . Effect of intracellular resuscitation of Legionella pneumophila in Acanthamoeba polyphage cells on the antimicrobial properties of silver and copper. Environ Sci Technol. 2006; 40(23):7434-9. DOI: 10.1021/es060412t. View

Pagnier I, Valles C, Raoult D, La Scola B . Isolation of Vermamoeba vermiformis and associated bacteria in hospital water. Microb Pathog. 2015; 80:14-20. DOI: 10.1016/j.micpath.2015.02.006. View

Walochnik J, Michel R, Aspock H . A molecular biological approach to the phylogenetic position of the genus Hyperamoeba. J Eukaryot Microbiol. 2004; 51(4):433-40. DOI: 10.1111/j.1550-7408.2004.tb00391.x. View

Atlan D, Coupat-Goutaland B, Risler A, Reyrolle M, Souchon M, Briolay J . Micriamoeba tesseris nov. gen. nov. sp.: a new taxon of free-living small-sized Amoebae non-permissive to virulent Legionellae. Protist. 2012; 163(6):888-902. DOI: 10.1016/j.protis.2012.04.006. View

Walochnik J, Obwaller A, Aspock H . Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl Environ Microbiol. 2000; 66(10):4408-13. PMC: 92317. DOI: 10.1128/AEM.66.10.4408-4413.2000. View

10.

Siddiqui R, Khan N . War of the microbial worlds: who is the beneficiary in Acanthamoeba-bacterial interactions?. Exp Parasitol. 2012; 130(4):311-3. DOI: 10.1016/j.exppara.2012.01.021. View

11.

Cirillo J, Cirillo S, Yan L, Bermudez L, Falkow S, Tompkins L . Intracellular growth in Acanthamoeba castellanii affects monocyte entry mechanisms and enhances virulence of Legionella pneumophila. Infect Immun. 1999; 67(9):4427-34. PMC: 96761. DOI: 10.1128/IAI.67.9.4427-4434.1999. View

12.

Walochnik J, Picher O, Aspock C, Ullmann M, Sommer R, Aspock H . Interactions of "Limax amoebae" and gram-negative bacteria: experimental studies and review of current problems. Tokai J Exp Clin Med. 2000; 23(6):273-8. View

13.

Rohr U, Weber S, Michel R, Selenka F, Wilhelm M . Comparison of free-living amoebae in hot water systems of hospitals with isolates from moist sanitary areas by identifying genera and determining temperature tolerance. Appl Environ Microbiol. 1998; 64(5):1822-4. PMC: 106236. DOI: 10.1128/AEM.64.5.1822-1824.1998. View

14.

Steinert M, Emody L, Amann R, Hacker J . Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii. Appl Environ Microbiol. 1997; 63(5):2047-53. PMC: 168494. DOI: 10.1128/aem.63.5.2047-2053.1997. View

15.

Ferre M, Arias C, Oliva J, Pedrol A, Garcia M, Pellicer T . A community outbreak of Legionnaires' disease associated with a cooling tower in Vic and Gurb, Catalonia (Spain) in 2005. Eur J Clin Microbiol Infect Dis. 2008; 28(2):153-9. DOI: 10.1007/s10096-008-0603-6. View

16.

Magnet A, Fenoy S, Galvan A, Izquierdo F, Rueda C, Fernandez Vadillo C . A year long study of the presence of free living amoeba in Spain. Water Res. 2013; 47(19):6966-72. DOI: 10.1016/j.watres.2013.09.065. View

17.

Scheikl U, Sommer R, Kirschner A, Rameder A, Schrammel B, Zweimuller I . Free-living amoebae (FLA) co-occurring with legionellae in industrial waters. Eur J Protistol. 2014; 50(4):422-9. PMC: 4155075. DOI: 10.1016/j.ejop.2014.04.002. View

18.

Ohno A, Kato N, Yamada K, Yamaguchi K . Factors influencing survival of Legionella pneumophila serotype 1 in hot spring water and tap water. Appl Environ Microbiol. 2003; 69(5):2540-7. PMC: 154533. DOI: 10.1128/AEM.69.5.2540-2547.2003. View

19.

De Jonckheere J . What do we know by now about the genus Naegleria?. Exp Parasitol. 2014; 145 Suppl:S2-9. DOI: 10.1016/j.exppara.2014.07.011. View

20.

Gast R, Fuerst P, Byers T . Discovery of group I introns in the nuclear small subunit ribosomal RNA genes of Acanthamoeba. Nucleic Acids Res. 1994; 22(4):592-6. PMC: 307848. DOI: 10.1093/nar/22.4.592. View