High Neuronal/astroglial Differentiation Plasticity of Adult Rat Hippocampal Neural Stem/progenitor Cells in Response to the Effects of Embryonic and Adult Cerebrospinal Fluids

Overview

Journal Iran J Vet Res

Specialty Veterinary Medicine

Date 2016 May 14

PMID 27175157

Citations 2

Authors

T Peirouvi

F Yekani

M Azarnia

M Massumi

Affiliations

Soon will be listed here.

Abstract

Hippocampal neural stem/progenitor cells (hipp-NS/PCs) of the adult mammalian brain are important sources of neuronal and gial cell production. In this study, the main goal is to investigate the plasticity of these cells in neuronal/astroglial differentiations. To this end, the differentiation of the hipp-NS/PCs isolated from 3-month-old Wistar rats was investigated in response to the embryonic cerebrospinal fluid (E-CSF) including E13.5, E17-CSF and the adult cerebrospinal fluid (A-CSF), all extracted from rats. CSF samples were selected based on their effects on cell behavioral parameters. Primary cell culture was performed in the presence of either normal or high levels of KCL in a culture medium. High levels of KCL cause cell depolarization, and thus the activation of quiescent NSCs. Results from immunocytochemistry (ICC) and semi-quantitative RT-PCR (sRT-PCR) techniques showed that in E-CSF-treated groups, neuronal differentiation increased (E17>E13.5). In contrast, A-CSF decreased and increased neuronal and astroglial differentiations, respectively. Cell survivability and/or proliferation (S/P), evaluated by an MTT assay, increased by E13.5 CSF, but decreased by both E17 CSF and A-CSF. Based on the results, it is finally concluded that adult rat hippocampal proliferative cells are not restricted progenitors but rather show high plasticity in neuronal/astroglial differentiation according to the effects of CSF samples. In addition, using high concentrations of KCL in the primary cell culture led to an increase in the number of NSCs, which in turn resulted in the increase in neuronal or astroglial differentiations after CSF treatment.

Citing Articles

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References

Zappaterra M, Lisgo S, Lindsay S, Gygi S, Walsh C, Ballif B . A comparative proteomic analysis of human and rat embryonic cerebrospinal fluid. J Proteome Res. 2007; 6(9):3537-48. DOI: 10.1021/pr070247w. View

Martin C, Alonso M, Santiago C, Moro J, de la Mano A, Carretero R . Early embryonic brain development in rats requires the trophic influence of cerebrospinal fluid. Int J Dev Neurosci. 2009; 27(7):733-40. DOI: 10.1016/j.ijdevneu.2009.06.002. View

van Praag H, Kempermann G, Gage F . Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus. Nat Neurosci. 1999; 2(3):266-70. DOI: 10.1038/6368. View

Palmer T, Takahashi J, Gage F . The adult rat hippocampus contains primordial neural stem cells. Mol Cell Neurosci. 1997; 8(6):389-404. DOI: 10.1006/mcne.1996.0595. View

Altman J, Das G . Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats. J Comp Neurol. 1965; 124(3):319-35. DOI: 10.1002/cne.901240303. View

Sun W, Winseck A, Vinsant S, Park O, Kim H, Oppenheim R . Programmed cell death of adult-generated hippocampal neurons is mediated by the proapoptotic gene Bax. J Neurosci. 2004; 24(49):11205-13. PMC: 6730275. DOI: 10.1523/JNEUROSCI.1436-04.2004. View

Bull N, Bartlett P . The adult mouse hippocampal progenitor is neurogenic but not a stem cell. J Neurosci. 2005; 25(47):10815-21. PMC: 6725873. DOI: 10.1523/JNEUROSCI.3249-05.2005. View

McDonald H, Martin Wojtowicz J . Dynamics of neurogenesis in the dentate gyrus of adult rats. Neurosci Lett. 2005; 385(1):70-5. DOI: 10.1016/j.neulet.2005.05.022. View

Reynolds B, Weiss S . Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science. 1992; 255(5052):1707-10. DOI: 10.1126/science.1553558. View

10.

Lois C, Alvarez-Buylla A . Proliferating subventricular zone cells in the adult mammalian forebrain can differentiate into neurons and glia. Proc Natl Acad Sci U S A. 1993; 90(5):2074-7. PMC: 46023. DOI: 10.1073/pnas.90.5.2074. View

11.

Montaron M, Drapeau E, Dupret D, Kitchener P, Aurousseau C, Le Moal M . Lifelong corticosterone level determines age-related decline in neurogenesis and memory. Neurobiol Aging. 2005; 27(4):645-54. DOI: 10.1016/j.neurobiolaging.2005.02.014. View

12.

Burgos K, Javaherian A, Bomprezzi R, Ghaffari L, Rhodes S, Courtright A . Identification of extracellular miRNA in human cerebrospinal fluid by next-generation sequencing. RNA. 2013; 19(5):712-22. PMC: 3677285. DOI: 10.1261/rna.036863.112. View

13.

Palmer T, Ray J, Gage F . FGF-2-responsive neuronal progenitors reside in proliferative and quiescent regions of the adult rodent brain. Mol Cell Neurosci. 1995; 6(5):474-86. DOI: 10.1006/mcne.1995.1035. View

14.

Hagihara H, Toyama K, Yamasaki N, Miyakawa T . Dissection of hippocampal dentate gyrus from adult mouse. J Vis Exp. 2009; (33). PMC: 3142893. DOI: 10.3791/1543. View

15.

Gould E, Beylin A, Tanapat P, Reeves A, Shors T . Learning enhances adult neurogenesis in the hippocampal formation. Nat Neurosci. 1999; 2(3):260-5. DOI: 10.1038/6365. View

16.

Garcia A, Steiner B, Kronenberg G, Bick-Sander A, Kempermann G . Age-dependent expression of glucocorticoid- and mineralocorticoid receptors on neural precursor cell populations in the adult murine hippocampus. Aging Cell. 2004; 3(6):363-71. DOI: 10.1111/j.1474-9728.2004.00130.x. View

17.

Carnicero E, Alonso M, Carretero R, Lamus F, Moro J, de la Mano A . Embryonic cerebrospinal fluid activates neurogenesis of neural precursors within the subventricular zone of the adult mouse brain. Cells Tissues Organs. 2014; 198(5):398-404. DOI: 10.1159/000356983. View

18.

Hattiangady B, Shetty A . Aging does not alter the number or phenotype of putative stem/progenitor cells in the neurogenic region of the hippocampus. Neurobiol Aging. 2006; 29(1):129-47. PMC: 3612500. DOI: 10.1016/j.neurobiolaging.2006.09.015. View

19.

Nabiuni M, Rasouli J, Parivar K, Kochesfehani H, Irian S, Miyan J . In vitro effects of fetal rat cerebrospinal fluid on viability and neuronal differentiation of PC12 cells. Fluids Barriers CNS. 2012; 9(1):8. PMC: 3386012. DOI: 10.1186/2045-8118-9-8. View

20.

Gato A, Moro J, Alonso M, Bueno D, de la Mano A, Martin C . Embryonic cerebrospinal fluid regulates neuroepithelial survival, proliferation, and neurogenesis in chick embryos. Anat Rec A Discov Mol Cell Evol Biol. 2005; 284(1):475-84. DOI: 10.1002/ar.a.20185. View