Fatty Acids Composition of Caenorhabditis Elegans Using Accurate Mass GCMS-QTOF

Overview

Journal J Environ Sci Health B

Specialties Environmental Health
Toxicology

Date 2016 May 12

PMID 27166662

Citations 8

Authors

Parise Henry

Olufunmilayo Owopetu

Demilade Adisa

Thao Nguyen

Kevin Anthony

David Ijoni-Animadu

Sakha Jamadar

Fawzia Abdel-Rahman

Mahmoud A Saleh

Affiliations

Soon will be listed here.

Abstract

The free living nematode Caenorhabditis elegans is a proven model organism for lipid metabolism research. Total lipids of C. elegans were extracted using chloroform and methanol in 2:1 ratio (v/v). Fatty acids composition of the extracted total lipids was converted to their corresponding fatty acids methyl esters (FAMEs) and analyzed by gas chromatography/accurate mass quadrupole time of flight mass spectrometry using both electron ionization and chemical ionization techniques. Twenty-eight fatty acids consisting of 12 to 22 carbon atoms were identified, 65% of them were unsaturated. Fatty acids containing 12 to17 carbons were mostly saturated with stearic acid (18:0) as the major constituent. Several branched-chain fatty acids were identified. Methyl-14-methylhexadecanoate (iso- 17:0) was the major identified branched fatty acid. This is the first report to detect the intact molecular parent ions of the identified fatty acids in C. elegans using chemical ionization compared to electron ionization which produced fragmentations of the FAMEs.

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