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Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Fails To Identify Nontuberculous Mycobacteria from Primary Cultures of Respiratory Samples

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2016 May 6
PMID 27147723
Citations 18
Authors
Kim van Eck
Dirk Faro
Melanie Wattenberg
Arjan de Jong
Saskia Kuipers
Jakko van Ingen
Affiliations
Soon will be listed here.
Abstract

We have assessed matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification (Bruker) of nontuberculous mycobacteria from newly positive liquid cultures of respiratory samples. Twelve (22%) of 54 isolates were identified directly from liquid medium. After subculture and with manual laser operation, this rose to 49/54 isolates (91%). MALDI-TOF MS is less promising than previously suggested.

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