Association of Kinase Insert Domain-containing Receptor (KDR) Gene Polymorphism/ Haplotypes with Recurrent Spontaneous Abortion and Genetic Structure

Overview

Journal Int J Reprod Biomed

Specialty Reproductive Medicine

Date 2016 May 4

PMID 27141535

Citations 5

Authors

Shiva Shahsavari

Zahra Noormohammadi

Shohreh Zare Karizi

Affiliations

Soon will be listed here.

Abstract

Background: Recurrent spontaneous abortion is one of the diseases that can lead to physical, psychological, and, economical problems for both individuals and society. Recently a few numbers of genetic polymorphisms in kinase insert domain-containing receptor (KDR) gene are examined that can endanger the life of the fetus in pregnant women.

Objective: The risk of KDR gene polymorphisms was investigated in Iranian women with idiopathic recurrent spontaneous abortion (RSA).

Materials And Methods: A case controlled study was performed. One hundred idiopathic recurrent spontaneous abortion patients with at least two consecutive pregnancy losses before 20 weeks of gestational age with normal karyotypes were included in the study. Also, 100 healthy women with at least one natural pregnancy were studied as control group. Two functional SNPs located in KDR gene; rs1870377 (Q472H), and rs2305948 (V297I) as well as one tag SNP in the intron region (rs6838752) were genotyped by using PCR based restriction fragment length polymorphism (PCR-RFLP) technique. Haplotype frequency was determined for these three SNPs' genotypes. Analysis of genetic STRUCTURE and K means clustering were performed to study genetic variation.

Results: Functional SNP (rs1870377) was highly linked to tag SNP (rs6838752) (D´ value=0. 214; χ(2) = 16.44, p<0. 001). K means clustering showed that k = 8 as the best fit for the optimal number of genetic subgroups in our studied materials. This result was in agreement with Neighbor Joining cluster analysis.

Conclusion: In our study, the allele and genotype frequencies were not associated with RSA between patient and control individuals. Inconsistent results in different populations with different allele frequencies among RSA patients and controls may be due to ethnic variation and used sample size.

Citing Articles

Association of KDR rs1870377 genotype with clopidogrel resistance in patients with post percutaneous coronary intervention.

Al Awaida W, Ahmed A, Hamza A, Amber K, Al-Ameer H, Jarrar Y Heliyon. 2021; 7(2):e06251.

PMID: 33665428 PMC: 7900686. DOI: 10.1016/j.heliyon.2021.e06251.

The Impact of Kinase Insert Domain (KDR) Gene Polymorphism rs2305948 on Clopidogrel Resistance in Iraqi Patients Undergoing Elective Percutaneous Coronary Intervention (PCI).

Ahmed A, Amber K, Hadi N Acta Inform Med. 2021; 28(3):202-208.

PMID: 33417661 PMC: 7780779. DOI: 10.5455/aim.2020.28.202-208.

Potential Role of Targeting KDR and Proteasome Inhibitors in the Therapy of Esophageal Squamous Cell Carcinoma.

Zhang L, Niu X, Bi Y, Cui H, Li H, Cheng X Technol Cancer Res Treat. 2020; 19:1533033820948060.

PMID: 32924793 PMC: 7493273. DOI: 10.1177/1533033820948060.

The association of Q472H variant in the gene with recurrent pregnancy loss in Southern Iran: A case-control study.

Keshavarz L, Yavarian M Int J Reprod Biomed. 2019; 17(7):473-480.

PMID: 31508572 PMC: 6718886. DOI: 10.18502/ijrm.v17i7.4858.

The Importance of VEGF-KDR Signaling Pathway Genes should Not Be Ignored When the Risk of Developing Multiple Sclerosis is Taken into Consideration.

Mazdeh M, Noroozi R, Gharesouran J, Sayad A, Komaki A, Eftekharian M J Mol Neurosci. 2017; 62(1):73-78.

PMID: 28401369 DOI: 10.1007/s12031-017-0912-2.

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