Parental Epigenetic Asymmetry of PRC2-mediated Histone Modifications in the Arabidopsis Endosperm

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2016 Apr 27

PMID 27113256

Citations 73

Authors

Jordi Moreno-Romero

Hua Jiang

Juan Santos-Gonzalez

Claudia Kohler

Affiliations

Soon will be listed here.

Abstract

Parental genomes in the endosperm are marked by differential DNA methylation and are therefore epigenetically distinct. This epigenetic asymmetry is established in the gametes and maintained after fertilization by unknown mechanisms. In this manuscript, we have addressed the key question whether parentally inherited differential DNA methylation affects de novo targeting of chromatin modifiers in the early endosperm. Our data reveal that polycomb-mediated H3 lysine 27 trimethylation (H3K27me3) is preferentially localized to regions that are targeted by the DNA glycosylase DEMETER (DME), mechanistically linking DNA hypomethylation to imprinted gene expression. Our data furthermore suggest an absence of de novo DNA methylation in the early endosperm, providing an explanation how DME-mediated hypomethylation of the maternal genome is maintained after fertilization. Lastly, we show that paternal-specific H3K27me3-marked regions are located at pericentromeric regions, suggesting that H3K27me3 and DNA methylation are not necessarily exclusive marks at pericentromeric regions in the endosperm.

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