Characterization, Expression and Silencing by RNAi of P53 from Penaeus Monodon

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2016 Apr 27

PMID 27112755

Citations 5

Authors

Wenting Dai

Lihua Qiu

Chao Zhao

Mingjun Fu

Zhenhua Ma

Falin Zhou

Qibin Yang

Affiliations

Soon will be listed here.

Abstract

The tumor suppressor p53 is a sequence-specific transcription factor, whose target genes can regulate genomic stability, the cellular response to DNA damage and cell-cycle progression. In the present study, the full-length complementary DNA (cDNA) sequence of p53 gene from Penaeus monodon (Pmp53) was cloned by the technology of rapid amplification of cDNA ends (RACE). The cDNA of Pmp53 was 2239 bp, encoding a protein of 450 amino acids with calculated molecular weight of 50.62 kDa. The temporal expression of Pmp53 in different tissues (ovary, heart, intestine, brain, muscles, stomach and gills) and different developmental stages of ovary was investigated by real-time quantitative PCR (RT-qPCR). The lowest expression level of Pmp53 was observed in the stomach, while the highest expression level was detected in the brain. During the ovary development stages, the expression level of Pmp53 reached the peak at stage III. RNA interference (RNAi) and serotonin (5-hydroxytryptamine, 5-HT) injection experiments were conducted to study the expression profile of Pmp53 and PmCDK2 (cyclin-dependent kinase 2, CDK2). Knocked down of Pmp53 by dsRNA-p53 was sequence-specific and successful. Expression levels of Pmp53 and PmCDK2 in ovary of P. monodon were significantly increased at 12-96 h post 5-HT injection. These results indicate that Pmp53 may be involved in the regulation of ovarian development of P. monodon.

Citing Articles

Molecular cloning, characterization and expression analysis of P53 from high latitude fish Phoxinus lagowskii and its response to hypoxia.

Wang J, Chen X, Ge X, Wang Z, Mu W Fish Physiol Biochem. 2022; 48(3):631-644.

PMID: 35411444 DOI: 10.1007/s10695-022-01072-6.

Effects of Dietary Clostridium butyricum on the Growth, Digestive Enzyme Activity, Antioxidant Capacity, and Resistance to Nitrite Stress of Penaeus monodon.

Duan Y, Zhang J, Huang J, Jiang S Probiotics Antimicrob Proteins. 2018; 11(3):938-945.

PMID: 29858778 DOI: 10.1007/s12602-018-9421-z.

Genomic structure, expression, and functional characterization of checkpoint kinase 1 from Penaeus monodon.

Qiu L, Zhao C, Wang P, Fan S, Yan L, Xie B PLoS One. 2018; 13(5):e0198036.

PMID: 29795680 PMC: 5967826. DOI: 10.1371/journal.pone.0198036.

Genomic structure, expression pattern, and functional characterization of transcription factor E2F-2 from black tiger shrimp (Penaeus monodon).

Xie B, Wang P, Zhao C, Qiu L PLoS One. 2017; 12(5):e0177420.

PMID: 28558060 PMC: 5448752. DOI: 10.1371/journal.pone.0177420.

Molecular cloning, characterization and expression analysis of a novel PDRG1 gene from black tiger shrimp (Penaeus monodon).

Zhao C, Dai W, Qiu L Genet Mol Biol. 2017; 40(1):93-103.

PMID: 28257526 PMC: 5409776. DOI: 10.1590/1678-4685-GMB-2016-0144.

References

Qiu G, Ramachandra R, Rexroad 3rd C, Yao J . Molecular characterization and expression profiles of cyclin B1, B2 and Cdc2 kinase during oogenesis and spermatogenesis in rainbow trout (Oncorhynchus mykiss). Anim Reprod Sci. 2007; 105(3-4):209-25. DOI: 10.1016/j.anireprosci.2007.03.005. View

Charoensapsri W, Amparyup P, Hirono I, Aoki T, Tassanakajon A . Gene silencing of a prophenoloxidase activating enzyme in the shrimp, Penaeus monodon, increases susceptibility to Vibrio harveyi infection. Dev Comp Immunol. 2009; 33(7):811-20. DOI: 10.1016/j.dci.2009.01.006. View

Bai H, Qiao H, Li F, Fu H, Sun S, Zhang W . Molecular characterization and developmental expression of vitellogenin in the oriental river prawn Macrobrachium nipponense and the effects of RNA interference and eyestalk ablation on ovarian maturation. Gene. 2014; 562(1):22-31. DOI: 10.1016/j.gene.2014.12.008. View

Lane D, CRAWFORD L . T antigen is bound to a host protein in SV40-transformed cells. Nature. 1979; 278(5701):261-3. DOI: 10.1038/278261a0. View

Schavolt K, Pietenpol J . p53 and Delta Np63 alpha differentially bind and regulate target genes involved in cell cycle arrest, DNA repair and apoptosis. Oncogene. 2007; 26(42):6125-32. DOI: 10.1038/sj.onc.1210441. View

Qiu L, Jiang S, Huang J, Wang W, Zhu C, Su T . Molecular cloning and mRNA expression of cyclophilin A gene in black tiger shrimp (Penaeus monodon). Fish Shellfish Immunol. 2008; 26(1):115-21. DOI: 10.1016/j.fsi.2008.03.022. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Lane D . Cancer. p53, guardian of the genome. Nature. 1992; 358(6381):15-6. DOI: 10.1038/358015a0. View

Chen J, Liu P, Li Z, Chen Y, Qiu G . The cloning of the cdk2 transcript and the localization of its expression during gametogenesis in the freshwater giant prawn, Macrobrachium rosenbergii. Mol Biol Rep. 2013; 40(8):4781-90. DOI: 10.1007/s11033-013-2574-7. View

10.

Visudtiphole V, Klinbunga S, Kirtikara K . Molecular characterization and expression profiles of cyclin A and cyclin B during ovarian development of the giant tiger shrimp Penaeus monodon. Comp Biochem Physiol A Mol Integr Physiol. 2009; 152(4):535-43. DOI: 10.1016/j.cbpa.2008.12.011. View

11.

El-Deiry W, Tokino T, Velculescu V, Levy D, Parsons R, Trent J . WAF1, a potential mediator of p53 tumor suppression. Cell. 1993; 75(4):817-25. DOI: 10.1016/0092-8674(93)90500-p. View

12.

Ongvarrasopone C, Chanasakulniyom M, Sritunyalucksana K, Panyim S . Suppression of PmRab7 by dsRNA inhibits WSSV or YHV infection in shrimp. Mar Biotechnol (NY). 2008; 10(4):374-81. DOI: 10.1007/s10126-007-9073-6. View

13.

Bunz F, Dutriaux A, Lengauer C, Waldman T, Zhou S, Brown J . Requirement for p53 and p21 to sustain G2 arrest after DNA damage. Science. 1998; 282(5393):1497-501. DOI: 10.1126/science.282.5393.1497. View

14.

Hollstein M, Sidransky D, Vogelstein B, Harris C . p53 mutations in human cancers. Science. 1991; 253(5015):49-53. DOI: 10.1126/science.1905840. View

15.

Liu M, Tee C, Zeng F, Sherry J, Dixon B, Bols N . Characterization of p53 expression in rainbow trout. Comp Biochem Physiol C Toxicol Pharmacol. 2011; 154(4):326-32. DOI: 10.1016/j.cbpc.2011.06.018. View

16.

Qiu G, Yamano K, Unuma T . Cathepsin C transcripts are differentially expressed in the final stages of oocyte maturation in kuruma prawn Marsupenaeus japonicus. Comp Biochem Physiol B Biochem Mol Biol. 2005; 140(2):171-81. DOI: 10.1016/j.cbpc.2004.09.027. View

17.

Pietenpol J, Tokino T, Thiagalingam S, El-Deiry W, Kinzler K, Vogelstein B . Sequence-specific transcriptional activation is essential for growth suppression by p53. Proc Natl Acad Sci U S A. 1994; 91(6):1998-2002. PMC: 43296. DOI: 10.1073/pnas.91.6.1998. View

18.

Tseng D, Chen Y, Kou G, Lo C, Kuo C . Hepatopancreas is the extraovarian site of vitellogenin synthesis in black tiger shrimp, Penaeus monodon. Comp Biochem Physiol A Mol Integr Physiol. 2001; 129(4):909-17. DOI: 10.1016/s1095-6433(01)00355-5. View

19.

Phinyo M, Visudtiphole V, Roytrakul S, Phaonakrop N, Jarayabhand P, Klinbunga S . Characterization and expression of cell division cycle 2 (Cdc2) mRNA and protein during ovarian development of the giant tiger shrimp Penaeus monodon. Gen Comp Endocrinol. 2013; 193:103-11. DOI: 10.1016/j.ygcen.2013.07.012. View

20.

Nevis K, Cordeiro-Stone M, Cook J . Origin licensing and p53 status regulate Cdk2 activity during G(1). Cell Cycle. 2009; 8(12):1952-63. PMC: 2972510. DOI: 10.4161/cc.8.12.8811. View