A CRISPR/Cas9 System Adapted for Gene Editing in Marine Algae

Overview

Journal Sci Rep

Specialty Science

Date 2016 Apr 26

PMID 27108533

Citations 106

Authors

Marianne Nymark

Amit Kumar Sharma

Torfinn Sparstad

Atle M Bones

Per Winge

Affiliations

Soon will be listed here.

Abstract

Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.

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