» Articles » PMID: 27098988

Posttranslational Hypusination of the Eukaryotic Translation Initiation Factor-5A Regulates Fusarium Graminearum Virulence

Overview
Journal Sci Rep
Specialty Science
Date 2016 Apr 22
PMID 27098988
Citations 10
Authors
Affiliations
Soon will be listed here.
Abstract

Activation of eukaryotic translation initiation factor eIF5A requires a posttranslational modification, forming the unique amino acid hypusine. This activation is mediated by two enzymes, deoxyhypusine synthase, DHS, and deoxyhypusine hydroxylase, DOHH. The impact of this enzymatic complex on the life cycle of a fungal pathogen is unknown. Plant pathogenic ascomycetes possess a single copy of the eIF5A activated by hypusination. We evaluated the importance of imbalances in eIF5A hypusination in Fusarium graminearum, a devastating fungal pathogen of cereals. Overexpression of DHS leads to increased virulence in wheat, elevated production of the mycotoxin deoxynivalenol, more infection structures, faster wheat tissue invasion in plants and increases vegetatively produced conidia. In contrast, overexpression of DOHH completely prevents infection structure formation, pathogenicity in wheat and maize, leads to overproduction of ROS, reduced DON production and increased sexual reproduction. Simultaneous overexpression of both genes restores wild type-like phenotypes. Analysis of eIF5A posttranslational modification displayed strongly increased hypusinated eIF5A in DOHH overexpression mutant in comparison to wild type, and the DHS overexpression mutants. These are the first results pointing to different functions of differently modified eIF5A.

Citing Articles

Diamine Fungal Inducers of Secondary Metabolism: 1,3-Diaminopropane and Spermidine Trigger Enzymes Involved in β-Alanine and Pantothenic Acid Biosynthesis, Precursors of Phosphopantetheine in the Activation of Multidomain Enzymes.

Martin J, Liras P Antibiotics (Basel). 2024; 13(9).

PMID: 39335000 PMC: 11428646. DOI: 10.3390/antibiotics13090826.


Update on the Basic Understanding of Virulence Factors in Common Wheat Research.

Buttar Z, Cheng M, Wei P, Zhang Z, Lv C, Zhu C Plants (Basel). 2024; 13(8).

PMID: 38674569 PMC: 11053692. DOI: 10.3390/plants13081159.


Hypusination, a Metabolic Posttranslational Modification of eIF5A in Plants during Development and Environmental Stress Responses.

Palfi P, Bakacsy L, Kovacs H, Szepesi A Plants (Basel). 2021; 10(7).

PMID: 34206171 PMC: 8309165. DOI: 10.3390/plants10071261.


Infection cushions of Fusarium graminearum are fungal arsenals for wheat infection.

Mentges M, Glasenapp A, Boenisch M, Malz S, Henrissat B, Frandsen R Mol Plant Pathol. 2020; 21(8):1070-1087.

PMID: 32573086 PMC: 7368127. DOI: 10.1111/mpp.12960.


Translation elongation factor P (EF-P).

Hummels K, Kearns D FEMS Microbiol Rev. 2020; 44(2):208-218.

PMID: 32011712 PMC: 7269679. DOI: 10.1093/femsre/fuaa003.


References
1.
Malonek S, Rojas M, Hedden P, Gaskin P, Hopkins P, Tudzynski B . The NADPH-cytochrome P450 reductase gene from Gibberella fujikuroi is essential for gibberellin biosynthesis. J Biol Chem. 2004; 279(24):25075-84. DOI: 10.1074/jbc.M308517200. View

2.
Lev S, Sharon A, Hadar R, Ma H, Horwitz B . A mitogen-activated protein kinase of the corn leaf pathogen Cochliobolus heterostrophus is involved in conidiation, appressorium formation, and pathogenicity: diverse roles for mitogen-activated protein kinase homologs in foliar pathogens. Proc Natl Acad Sci U S A. 1999; 96(23):13542-7. PMC: 23984. DOI: 10.1073/pnas.96.23.13542. View

3.
Schrader R, Young C, Kozian D, Hoffmann R, Lottspeich F . Temperature-sensitive eIF5A mutant accumulates transcripts targeted to the nonsense-mediated decay pathway. J Biol Chem. 2006; 281(46):35336-46. DOI: 10.1074/jbc.M601460200. View

4.
Park J, Aravind L, Wolff E, Kaevel J, Kim Y, Park M . Molecular cloning, expression, and structural prediction of deoxyhypusine hydroxylase: a HEAT-repeat-containing metalloenzyme. Proc Natl Acad Sci U S A. 2005; 103(1):51-6. PMC: 1324997. DOI: 10.1073/pnas.0509348102. View

5.
Maier F, Miedaner T, Hadeler B, Felk A, Salomon S, Lemmens M . Involvement of trichothecenes in fusarioses of wheat, barley and maize evaluated by gene disruption of the trichodiene synthase (Tri5) gene in three field isolates of different chemotype and virulence. Mol Plant Pathol. 2010; 7(6):449-61. DOI: 10.1111/j.1364-3703.2006.00351.x. View