Thioflavin T As an Efficient Fluorescence Sensor for Selective Recognition of RNA G-quadruplexes

Overview

Journal Sci Rep

Specialty Science

Date 2016 Apr 22

PMID 27098781

Citations 62

Authors

Shujuan Xu

Qian Li

Junfeng Xiang

Qianfan Yang

Hongxia Sun

Aijiao Guan

Lixia Wang

Yan Liu

Lijia Yu

Yunhua Shi

Hongbo Chen

Yalin Tang

Affiliations

Soon will be listed here.

Abstract

RNA G-quadruplexes (G4s) play important roles in translational regulation, mRNA processing events and gene expression. Therefore, a fluorescent probe that is capable of efficiently recognizing RNA G-quadruplex structures among other RNA forms is highly desirable. In this study, a water-soluble fluorogenic dye (i.e., Thioflavin T (ThT)) was employed to recognize RNA G-quadruplex structures using UV-Vis absorption spectra, fluorescence spectra and emission lifetime experiments. By stacking on the G-tetrad, the ThT probe exhibited highly specific recognition of RNA G-quadruplex structures with striking fluorescence enhancement compared with other RNA forms. The specific binding demonstrates that ThT is an efficient fluorescence sensor that can distinguish G4 and non-G4 RNA structures.

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