Study on Mycoflora of Poultry Feed Ingredients and Finished Feed in Iran

Overview

Journal Iran J Microbiol

Publisher Tehran University of Medical Sciences

Specialty Infectious Diseases

Date 2016 Apr 20

PMID 27092224

Citations 2

Authors

Seyed Soheil Ghaemmaghami

Mehrdad Modirsaneii

Ali Reza Khosravi

Mehdi Razzaghi-Abyaneh

Affiliations

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Abstract

Background And Objectives: Unhygienic poultry feedstuffs can lead to nutrient losses and detrimental effect on poultry production and public health. In the present study, mycobiota and colony-forming units per gram in ingredients and finish poultry feed was evaluated with special reference to potentially mycotoxigenic fungi.

Materials And Methods: Eighty five samples of corn, soybean meal and poultry finished feed were collected from nine poultry feed factories located in three provinces i.e. Tehran, Alborz and Qom in Iran from October 2014 to January 2015. Samples were cultured on Sabouraud dextrose agar (SDA), Aspergillus flavus and parasiticus agar (AFPA) and dichloran rosebengal chloramphenicol agar (DRBC) and incubated at 28 °C for 7-10 days. Purified fungal colonies were identified by a combination of macro- and microscopic morphological criteria. For determining the rate of fungal contamination, samples were cultured on SDA and colony forming units (CFUs) were calculated.

Results: A total of 384 fungal isolates belonging to 7 genera of filamentous fungi and yeasts were obtained from corn (124 isolates), soybean meal (92 isolates), and feed before (72 isolates), and after pelleting (96 isolates). The most prominent fungal isolate in corn, soybean meal and feed before pelleting (feed as mash form) was Fusarium but in feed after pelleting was Aspergillus. Among 5 Aspergillus species isolated, potentially aflatoxigenic A. flavus isolates was predominant in corn (46.6%), soybean meal (72.7%) and poultry finished feed (75%). CFUs results indicated that 9/22 corn samples (40.9%), none of 22 soybean meal samples, 19/41 finished feed (46.3%) were contaminated higher than the standard limit.

Conclusions: Our results indicated that corn, soybean meal and finished feed of poultry feed mill are contaminated with various fungal genera by different levels sometimes higher that the standard limits. Contamination with potentially mycotoxigenic fungi especially Aspergillus species may be considered as a human public health hazard.

Citing Articles

Perturbations of the ileal mycobiota by necrotic enteritis in broiler chickens.

Yang Q, Liu J, Robinson K, Whitmore M, Stewart S, Zhang G J Anim Sci Biotechnol. 2021; 12(1):107.

PMID: 34625122 PMC: 8501549. DOI: 10.1186/s40104-021-00628-5.

Toxigenicity and phylogeny of section in poultry feed in Iran.

Ghaemmaghami S, Pashootan N, Razzaghi-Abyaneh M Curr Med Mycol. 2020; 6(1):22-29.

PMID: 32420504 PMC: 7217249. DOI: 10.18502/cmm.6.1.2504.

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