Growth Suppression Effect of Human Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue, and Wharton's Jelly of Umbilical Cord on PBMCs

Overview

Journal Iran J Basic Med Sci

Publisher Mashhad University of Medical Sciences

Specialty General Medicine

Date 2016 Apr 16

PMID 27081458

Citations 17

Authors

Maryam Ayatollahi

Tahereh Talaei-Khozani

Mahboobeh Razmkhah

Affiliations

Soon will be listed here.

Abstract

Objectives: Immunosuppressive property of mesenchymal stem cells (MSCs) has great attraction in regenerative medicine especially when dealing with tissue damage involving immune reactions. The most attractive tissue sources of MSCs used in clinical applications are bone marrow (BM), adipose tissue (AT), and Wharton's jelly (WJ) of human umbilical cord. The current study has compared immunomodulatory properties of human BM, AT, and WJ-MSCs.

Materials And Methods: Three different types of human MSCs were isolated, cultured, and characterized by flow cytometry and differentiation potentials. The MSCs were co-cultured with allogeneic phytohemagglutinin (PHA) activated peripheral blood mononuclear cells (PBMCs). The proliferation of PBMCs was assessed by flow cytometry of carboxyfluorescein succinimidyl ester (CFSE) stained cells and compared to each other and to the growth of PBMCs in the absence of MSCs. Additionally, the growth suppression was indirectly assessed by using the transwell culture system.

Results: The proliferation of PBMCs reduced to 6.2, 7 and 15.4- fold in cultures with AT-MSCs, WJ-MSCs, and BM-MSCs, respectively, compared to the PHA-activated cells. When the growth suppression was indirectly assessed by using the transwell culture system, it was revealed that AT-MSCs, WJ-MSCs, and BM-MSCs caused growth reduction in PBMCs to 3, 8, and 8 -fold, respectively, compared to the PHA-activated cells.

Conclusion: These data collectively conclude that the immunomodulatory effects of MSCs, which may mostly carry out through direct cell to cell contact, are different between various sources. Accordingly results of this study may contribute to the application of these cells in cell therapy and regenerative medicine.

Citing Articles

Wharton's Jelly Mesenchymal Stem Cell Conditioned Medium Ameliorates Diabetes-Induced Testicular Damage and Sperm Abnormalities by Mitigating Oxidative Stress, Apoptosis, and Inflammation.

Sargazi M, Karbalaei N, Karbalay-Doust S, Keshtgar S, Aghaei Z Stem Cells Int. 2024; 2024:7084913.

PMID: 39391856 PMC: 11466552. DOI: 10.1155/2024/7084913.

Factors Defining Human Adipose Stem/Stromal Cell Immunomodulation in Vitro.

Mahmoud M, Abdel-Rasheed M, Galal E, R El-Awady R Stem Cell Rev Rep. 2023; 20(1):175-205.

PMID: 37962697 PMC: 10799834. DOI: 10.1007/s12015-023-10654-7.

Efficacy and safety of allogeneic umbilical cord-derived mesenchymal stem cells for the treatment of complex perianal fistula in Crohn's disease: a pilot study.

Wei J, Zhang Y, Chen C, Feng X, Yang Z, Feng J Stem Cell Res Ther. 2023; 14(1):311.

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Systematic review and meta-analysis of cell therapy for COVID-19: global clinical trial landscape, published safety/efficacy outcomes, cell product manufacturing and clinical delivery.

Couto P, Al-Arawe N, Filgueiras I, Fonseca D, Hinterseher I, Catar R Front Immunol. 2023; 14:1200180.

PMID: 37415976 PMC: 10321603. DOI: 10.3389/fimmu.2023.1200180.

Neuroprotective Effect of Wharton's Jelly-Derived Mesenchymal Stem Cell-Conditioned Medium (WJMSC-CM) on Diabetes-Associated Cognitive Impairment by Improving Oxidative Stress, Neuroinflammation, and Apoptosis.

Aghaei Z, Karbalaei N, Namavar M, Haghani M, Razmkhah M, Ghaffari M Stem Cells Int. 2023; 2023:7852394.

PMID: 37081849 PMC: 10113062. DOI: 10.1155/2023/7852394.

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