Endemicity of Zoonotic Diseases in Pigs and Humans in Lowland and Upland Lao PDR: Identification of Socio-cultural Risk Factors

Overview

Journal PLoS Negl Trop Dis

Specialties Infectious Diseases
Tropical Medicine

Date 2016 Apr 13

PMID 27070428

Citations 29

Authors

Hannah R Holt

Phouth Inthavong

Boualam Khamlome

Kate Blaszak

Chattouphone Keokamphe

Virasack Somoulay

Anousone Phongmany

Peter A Durr

Kerryne Graham

John Allen

Blanaid Donnelly

Stuart D Blacksell

Fred Unger

Delia Grace

Silvia Alonso

Jeff Gilbert

Affiliations

Soon will be listed here.

Abstract

In Lao People's Democratic Republic pigs are kept in close contact with families. Human risk of infection with pig zoonoses arises from direct contact and consumption of unsafe pig products. This cross-sectional study was conducted in Luang Prabang (north) and Savannakhet (central-south) Provinces. A total of 59 villages, 895 humans and 647 pigs were sampled and serologically tested for zoonotic pathogens including: hepatitis E virus (HEV), Japanese encephalitis virus (JEV) and Trichinella spiralis; In addition, human sera were tested for Taenia spp. and cysticercosis. Seroprevalence of zoonotic pathogens in humans was high for HEV (Luang Prabang: 48.6%, Savannakhet: 77.7%) and T. spiralis (Luang Prabang: 59.0%, Savannakhet: 40.5%), and lower for JEV (around 5%), Taenia spp. (around 3%) and cysticercosis (Luang Prabang: 6.1, Savannakhet 1.5%). Multiple correspondence analysis and hierarchical clustering of principal components was performed on descriptive data of human hygiene practices, contact with pigs and consumption of pork products. Three clusters were identified: Cluster 1 had low pig contact and good hygiene practices, but had higher risk of T. spiralis. Most people in cluster 2 were involved in pig slaughter (83.7%), handled raw meat or offal (99.4%) and consumed raw pigs' blood (76.4%). Compared to cluster 1, cluster 2 had increased odds of testing seropositive for HEV and JEV. Cluster 3 had the lowest sanitation access and had the highest risk of HEV, cysticercosis and Taenia spp. Farmers which kept their pigs tethered (as opposed to penned) and disposed of manure in water sources had 0.85 (95% CI: 0.18 to 0.91) and 2.39 (95% CI: 1.07 to 5.34) times the odds of having pigs test seropositive for HEV, respectively. The results have been used to identify entry-points for intervention and management strategies to reduce disease exposure in humans and pigs, informing control activities in a cysticercosis hyper-endemic village.

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References

Handali S, Klarman M, Gaspard A, Noh J, Lee Y, Rodriguez S . Multiantigen print immunoassay for comparison of diagnostic antigens for Taenia solium cysticercosis and taeniasis. Clin Vaccine Immunol. 2009; 17(1):68-72. PMC: 2812080. DOI: 10.1128/CVI.00339-09. View

Schantz P, Shanks D, Wilson M . Serologic cross-reactions with sera from patients with echinococcosis and cysticercosis. Am J Trop Med Hyg. 1980; 29(4):609-12. DOI: 10.4269/ajtmh.1980.29.609. View

Conlan J, Jarman R, Vongxay K, Chinnawirotpisan P, Melendrez M, Fenwick S . Hepatitis E virus is prevalent in the pig population of Lao People's Democratic Republic and evidence exists for homogeneity with Chinese Genotype 4 human isolates. Infect Genet Evol. 2011; 11(6):1306-11. DOI: 10.1016/j.meegid.2011.04.022. View

Ito A, Nakao M, Wandra T . Human Taeniasis and cysticercosis in Asia. Lancet. 2003; 362(9399):1918-20. DOI: 10.1016/S0140-6736(03)14965-3. View

Bruschi F . Trichinellosis in developing countries: is it neglected?. J Infect Dev Ctries. 2012; 6(3):216-22. DOI: 10.3855/jidc.2478. View

Meng X . Hepatitis E virus: animal reservoirs and zoonotic risk. Vet Microbiol. 2009; 140(3-4):256-65. PMC: 2814965. DOI: 10.1016/j.vetmic.2009.03.017. View

Conlan J, Vongxay K, Jarman R, Gibbons R, Lunt R, Fenwick S . Serologic study of pig-associated viral zoonoses in Laos. Am J Trop Med Hyg. 2012; 86(6):1077-1084. PMC: 3366526. DOI: 10.4269/ajtmh.2012.11-0195. View

Salim L, Ang A, Handali S, Tsang V . Seroepidemiologic survey of cysticercosis-taeniasis in four central highland districts of Papua, Indonesia. Am J Trop Med Hyg. 2009; 80(3):384-8. View

Dohoo I, Ducrot C, Fourichon C, Donald A, Hurnik D . An overview of techniques for dealing with large numbers of independent variables in epidemiologic studies. Prev Vet Med. 1997; 29(3):221-39. DOI: 10.1016/s0167-5877(96)01074-4. View

10.

Conlan J, Khounsy S, Inthavong P, Fenwick S, Blacksell S, Thompson R . A review of taeniasis and cysticercosis in the Lao People's Democratic Republic. Parasitol Int. 2008; 57(3):252-5. DOI: 10.1016/j.parint.2008.04.002. View

11.

Bardosh K, Inthavong P, Xayaheuang S, Okello A . Controlling parasites, understanding practices: the biosocial complexity of a One Health intervention for neglected zoonotic helminths in northern Lao PDR. Soc Sci Med. 2014; 120:215-23. DOI: 10.1016/j.socscimed.2014.09.030. View

12.

Willingham 3rd A, Wu H, Conlan J, Satrija F . Combating Taenia solium cysticercosis in Southeast Asia an opportunity for improving human health and livestock production. Adv Parasitol. 2010; 72:235-66. DOI: 10.1016/S0065-308X(10)72009-1. View

13.

Somphou P, Takano T, Nakamura K . Cohabitation with farm animals in urban households with and without occupational farm work: associations between participation in educational activities and good hygiene practices in at-risk households cohabiting with farm animals. Environ Health Prev Med. 2009; 13(6):322-31. PMC: 2698226. DOI: 10.1007/s12199-008-0046-9. View

14.

Meng X, Wiseman B, Elvinger F, Guenette D, Toth T, Engle R . Prevalence of antibodies to hepatitis E virus in veterinarians working with swine and in normal blood donors in the United States and other countries. J Clin Microbiol. 2002; 40(1):117-22. PMC: 120098. DOI: 10.1128/JCM.40.1.117-122.2002. View

15.

Jacobson J, Hills S, Winkler J, Mammen M, Thaisomboonsuk B, Marfin A . Evaluation of three immunoglobulin M antibody capture enzyme-linked immunosorbent assays for diagnosis of Japanese encephalitis. Am J Trop Med Hyg. 2007; 77(1):164-8. View

16.

Frey C, Buholzer P, Beck R, Marinculic A, Raeber A, Gottstein B . Evaluation of a new commercial enzyme-linked immunosorbent assay for the detection of porcine antibodies against Trichinella spp. J Vet Diagn Invest. 2009; 21(5):692-7. DOI: 10.1177/104063870902100516. View

17.

Barennes H, Sayasone S, Odermatt P, De Bruyne A, Hongsakhone S, Newton P . A major trichinellosis outbreak suggesting a high endemicity of Trichinella infection in northern Laos. Am J Trop Med Hyg. 2008; 78(1):40-4. PMC: 7611085. View

18.

Kumar S, Subhadra S, Singh B, Panda B . Hepatitis E virus: the current scenario. Int J Infect Dis. 2013; 17(4):e228-33. DOI: 10.1016/j.ijid.2012.11.026. View

19.

Conlan J, Sripa B, Attwood S, Newton P . A review of parasitic zoonoses in a changing Southeast Asia. Vet Parasitol. 2011; 182(1):22-40. DOI: 10.1016/j.vetpar.2011.07.013. View

20.

Steyer A, Naglic T, Mocilnik T, Poljsak-Prijatelj M, Poljak M . Hepatitis E virus in domestic pigs and surface waters in Slovenia: prevalence and molecular characterization of a novel genotype 3 lineage. Infect Genet Evol. 2011; 11(7):1732-7. DOI: 10.1016/j.meegid.2011.07.007. View