Biotechnology and Apple Breeding in Japan

Overview

Journal Breed Sci

Date 2016 Apr 13

PMID 27069388

Citations 10

Authors

Megumi Igarashi

Yoshimichi Hatsuyama

Takeo Harada

Tomoko Fukasawa-Akada

Affiliations

Soon will be listed here.

Abstract

Apple is a fruit crop of significant economic importance, and breeders world wide continue to develop novel cultivars with improved characteristics. The lengthy juvenile period and the large field space required to grow apple populations have imposed major limitations on breeding. Various molecular biological techniques have been employed to make apple breeding easier. Transgenic technology has facilitated the development of apples with resistance to fungal or bacterial diseases, improved fruit quality, or root stocks with better rooting or dwarfing ability. DNA markers for disease resistance (scab, powdery mildew, fire-blight, Alternaria blotch) and fruit skin color have also been developed, and marker-assisted selection (MAS) has been employed in breeding programs. In the last decade, genomic sequences and chromosome maps of various cultivars have become available, allowing the development of large SNP arrays, enabling efficient QTL mapping and genomic selection (GS). In recent years, new technologies for genetic improvement, such as trans-grafting, virus vectors, and genome-editing, have emerged. Using these techniques, no foreign genes are present in the final product, and some of them show considerable promise for application to apple breeding.

Citing Articles

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References

Murata M, Haruta M, Murai N, Tanikawa N, Nishimura M, Homma S . Transgenic apple (Malus x domestica) shoot showing low browning potential. J Agric Food Chem. 2000; 48(11):5243-8. DOI: 10.1021/jf000771m. View

Holefors A, Ahlman A, Welander M . Transformation of the apple rootstock M.9/29 with the rolB gene and its influence on rooting and growth. Plant Sci. 2001; 160(3):433-439. DOI: 10.1016/s0168-9452(00)00401-5. View

Vinatzer B, Patocchi A, Gianfranceschi L, Tartarini S, Zhang H, Gessler C . Apple contains receptor-like genes homologous to the Cladosporium fulvum resistance gene family of tomato with a cluster of genes cosegregating with Vf apple scab resistance. Mol Plant Microbe Interact. 2001; 14(4):508-15. DOI: 10.1094/MPMI.2001.14.4.508. View

Bolar J, Norelli J, Harman G, Brown S, Aldwinckle H . Synergistic activity of endochitinase and exochitinase from Trichoderma atroviride (T. harzianum) against the pathogenic fungus (Venturia inaequalis) in transgenic apple plants. Transgenic Res. 2002; 10(6):533-43. DOI: 10.1023/a:1013036732691. View

Terras F, Schoofs H, Thevissen K, OSBORN R, Vanderleyden J, Cammue B . Synergistic Enhancement of the Antifungal Activity of Wheat and Barley Thionins by Radish and Oilseed Rape 2S Albumins and by Barley Trypsin Inhibitors. Plant Physiol. 1993; 103(4):1311-1319. PMC: 159121. DOI: 10.1104/pp.103.4.1311. View

Belfanti E, Silfverberg-Dilworth E, Tartarini S, Patocchi A, Barbieri M, Zhu J . The HcrVf2 gene from a wild apple confers scab resistance to a transgenic cultivated variety. Proc Natl Acad Sci U S A. 2004; 101(3):886-90. PMC: 321776. DOI: 10.1073/pnas.0304808101. View

James C, Clarke J, Evans K . Identification of molecular markers linked to the mildew resistance gene Pl-d in apple. Theor Appl Genet. 2004; 110(1):175-81. DOI: 10.1007/s00122-004-1836-0. View

Haywood V, Yu T, Huang N, Lucas W . Phloem long-distance trafficking of GIBBERELLIC ACID-INSENSITIVE RNA regulates leaf development. Plant J. 2005; 42(1):49-68. DOI: 10.1111/j.1365-313X.2005.02351.x. View

Li H, Flachowsky H, Fischer T, Hanke M, Forkmann G, Treutter D . Maize Lc transcription factor enhances biosynthesis of anthocyanins, distinct proanthocyanidins and phenylpropanoids in apple (Malus domestica Borkh.). Planta. 2007; 226(5):1243-54. DOI: 10.1007/s00425-007-0573-4. View

10.

Khan M, Durel C, Duffy B, Drouet D, Kellerhals M, Gessler C . Development of molecular markers linked to the 'Fiesta' linkage group 7 major QTL for fire blight resistance and their application for marker-assisted selection. Genome. 2007; 50(6):568-77. DOI: 10.1139/g07-033. View

11.

Bolar J, Norelli J, Wong K, Hayes C, Harman G, Aldwinckle H . Expression of Endochitinase from Trichoderma harzianum in Transgenic Apple Increases Resistance to Apple Scab and Reduces Vigor. Phytopathology. 2008; 90(1):72-7. DOI: 10.1094/PHYTO.2000.90.1.72. View

12.

Benaouf G, Parisi L . Genetics of Host-Pathogen Relationships Between Venturia inaequalis Races 6 and 7 and Malus Species. Phytopathology. 2008; 90(3):236-42. DOI: 10.1094/PHYTO.2000.90.3.236. View

13.

Durel C, Denance C, Brisset M . Two distinct major QTL for resistance to fire blight co-localize on linkage group 12 in apple genotypes 'Evereste' and Malus floribunda clone 821. Genome. 2009; 52(2):139-47. DOI: 10.1139/g08-111. View

14.

Smolka A, Li X, Heikelt C, Welander M, Zhu L . Effects of transgenic rootstocks on growth and development of non-transgenic scion cultivars in apple. Transgenic Res. 2010; 19(6):933-48. DOI: 10.1007/s11248-010-9370-0. View

15.

Kotoda N, Hayashi H, Suzuki M, Igarashi M, Hatsuyama Y, Kidou S . Molecular characterization of FLOWERING LOCUS T-like genes of apple (Malus x domestica Borkh.). Plant Cell Physiol. 2010; 51(4):561-75. DOI: 10.1093/pcp/pcq021. View

16.

Galli P, Patocchi A, Broggini G, Gessler C . The Rvi15 (Vr2) apple scab resistance locus contains three TIR-NBS-LRR genes. Mol Plant Microbe Interact. 2010; 23(5):608-17. DOI: 10.1094/MPMI-23-5-0608. View

17.

Costa F, Peace C, Stella S, Serra S, Musacchi S, Bazzani M . QTL dynamics for fruit firmness and softening around an ethylene-dependent polygalacturonase gene in apple (Malus x domestica Borkh.). J Exp Bot. 2010; 61(11):3029-39. PMC: 2892147. DOI: 10.1093/jxb/erq130. View

18.

Borejsza-Wysocka E, Norelli J, Aldwinckle H, Malnoy M . Stable expression and phenotypic impact of attacin E transgene in orchard grown apple trees over a 12 year period. BMC Biotechnol. 2010; 10:41. PMC: 2910661. DOI: 10.1186/1472-6750-10-41. View

19.

Velasco R, Zharkikh A, Affourtit J, Dhingra A, Cestaro A, Kalyanaraman A . The genome of the domesticated apple (Malus × domestica Borkh.). Nat Genet. 2010; 42(10):833-9. DOI: 10.1038/ng.654. View

20.

Yamagishi N, Sasaki S, Yamagata K, Komori S, Nagase M, Wada M . Promotion of flowering and reduction of a generation time in apple seedlings by ectopical expression of the Arabidopsis thaliana FT gene using the Apple latent spherical virus vector. Plant Mol Biol. 2010; 75(1-2):193-204. DOI: 10.1007/s11103-010-9718-0. View