Replication of Adenovirus Type 2 DNA in Vitro

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Oct 1

PMID 270687

Citations 12

Authors

L M Kaplan

R E Kelinman

M S Horwitz

Affiliations

Soon will be listed here.

Abstract

A soluble replication system that synthesizes full-sized adenovirus DNA molecules has been developed. The extraction of infected HeLa nuclei solubilizes approximately 25% of the viral replicating DNA and leaves the HeLa chromatin associated with the insoluble nuclear material. Both the extracted viral DNA and the DNA product synthesized in vitro are intact and identical to the adenovirus DNA or its replicating intermediates produced in whole infected cells. The direction of elongation and the termini of replication of progeny DNA in vitro are essentially identical to those observed in vivo. Nuclear extract replication is dependent on Mg2+ and the four deoxyribonucleoside triphosphates and partially stimulated by ATP.

Citing Articles

Chinese hamster ovary cells replicate adenovirus deoxyribonucleic acid.

Longiaru M, Horwitz M Mol Cell Biol. 1981; 1(3):208-15.

PMID: 6965097 PMC: 369664. DOI: 10.1128/mcb.1.3.208-215.1981.

Coordinate regulation of two cytoplasmic RNA species transcribed from early region 2 of the adenovirus 2 genome.

Goldenberg C, Rosenthal R, Bhaduri S, Raskas H J Virol. 1981; 38(3):932-9.

PMID: 6894621 PMC: 171231. DOI: 10.1128/JVI.38.3.932-939.1981.

DNA polymerase requirements for parvovirus H-1 DNA replication in vitro.

Kollek R, Tseng B, Goulian M J Virol. 1982; 41(3):982-9.

PMID: 6808155 PMC: 256835. DOI: 10.1128/JVI.41.3.982-989.1982.

Herpes simplex virus types 1 and 2 completely help adenovirus-associated virus replication.

Buller R, Janik J, SEBRING E, Rose J J Virol. 1981; 40(1):241-7.

PMID: 6270377 PMC: 256613. DOI: 10.1128/JVI.40.1.241-247.1981.

Multiple rounds of adenovirus DNA synthesis in vitro.

Horwitz M, Ariga H Proc Natl Acad Sci U S A. 1981; 78(3):1476-80.

PMID: 6262804 PMC: 319153. DOI: 10.1073/pnas.78.3.1476.

References

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Knopf K, Weissbach A . Study of DNA synthesis in chromatin isolated from HeLa cells. Biochemistry. 1977; 16(14):3190-4. DOI: 10.1021/bi00633a023. View

Rekosh D, Russell W, Bellet A, Robinson A . Identification of a protein linked to the ends of adenovirus DNA. Cell. 1977; 11(2):283-95. DOI: 10.1016/0092-8674(77)90045-9. View

Su R, DePamphilis M . In vitro replication of simian virus 40 DNA in a nucleoprotein complex. Proc Natl Acad Sci U S A. 1976; 73(10):3466-70. PMC: 431136. DOI: 10.1073/pnas.73.10.3466. View

Edenberg H, Waqar M, Huberman J . Subnuclear systems for synthesis of simian virus 40 DNA in vitro. Proc Natl Acad Sci U S A. 1976; 73(12):4392-6. PMC: 431470. DOI: 10.1073/pnas.73.12.4392. View

Sussenbach J, Kuijk M . Studies on the mechanism of replication of adenovirus DNA. V. The location of termini of replication. Virology. 1977; 77(1):149-57. DOI: 10.1016/0042-6822(77)90414-7. View

Flint S, Berget S, Sharp P . Characterization of single-stranded viral DNA sequences present during replication of adenovirus types 2 and 5. Cell. 1976; 9(4 Pt 1):559-71. DOI: 10.1016/0092-8674(76)90038-6. View

Weingartner B, Winnacker E, Tolun A, Pettersson U . Two complementary strand-specific termination sites for adenovirus DNA replication. Cell. 1976; 9(2):259-68. DOI: 10.1016/0092-8674(76)90117-3. View

Sussenbach J, van der Vliet P, Ellens D, Jansz H . Linear intermediates in the replication of adenovirus DNA. Nat New Biol. 1972; 239(89):47-9. View

10.

Horwitz M . Location of the origin of DNA replication in adenovirus Type 2. J Virol. 1974; 13(5):1046-54. PMC: 355413. DOI: 10.1128/JVI.13.5.1046-1054.1974. View

11.

Horwitz M, Brayton C, Baum S . Synthesis of type 2 adenovirus DNA in the presence of cycloheximide. J Virol. 1973; 11(4):544-51. PMC: 355136. DOI: 10.1128/JVI.11.4.544-551.1973. View

12.

Winnacker E, Magnusson G, REICHARD P . Replication of polyoma DNA in isolated nuclei. I. Characterization of the system from mouse fibroblast 3T6 cells. J Mol Biol. 1972; 72(3):523-37. DOI: 10.1016/0022-2836(72)90172-6. View

13.

Hershey H, Taylor J . DNA replication in eucaryotic nuclei. Evidence suggesting a specific model of replication. Exp Cell Res. 1974; 85(1):79-88. DOI: 10.1016/0014-4827(74)90215-8. View

14.

Wilhelm J, Brison O, Kedinger C, Chambon P . Characterization of adenovirus type 2 transcriptional complexes isolated from infected HeLa cell nuclei. J Virol. 1976; 19(1):61-81. PMC: 354833. DOI: 10.1128/JVI.19.1.61-81.1976. View

15.

Krokan H, Bjorklid E, Prydz H . DNA synthesis in isolated HeLa cell nuclei. Optimalization of the system and characterization of the product. Biochemistry. 1975; 14(19):4227-32. DOI: 10.1021/bi00690a012. View

16.

Garon C, Berry K, Rose J . A unique form of terminal redundancy in adenovirus DNA molecules. Proc Natl Acad Sci U S A. 1972; 69(9):2391-5. PMC: 426947. DOI: 10.1073/pnas.69.9.2391. View

17.

Wolfson J, Dressler D . Adenovirus-2 DNA contains an inverted terminal repetition. Proc Natl Acad Sci U S A. 1972; 69(10):3054-7. PMC: 389705. DOI: 10.1073/pnas.69.10.3054. View

18.

Yamashita T, Arens M, Green M . Adenovirus deoxyribonucleic acid replication. II. Synthesis of viral deoxyribonucleic acid in vitro by a nuclear membrane fraction from infected KB cells. J Biol Chem. 1975; 250(9):3273-9. View

19.

DePamphilis M, Berg P . Requirement of a Cytoplasmic Fraction for Synthesis of SV40 Deoxyribonucleic Acid in Isolated Nuclei*. J Biol Chem. 1975; 250(11):4348-54. View

20.

Robinson A, Bellett J . A circular DNA-protein complex adenoviruses and its possible role in DNA replication. Cold Spring Harb Symp Quant Biol. 1975; 39 Pt 1:523-31. DOI: 10.1101/sqb.1974.039.01.064. View