Photoleucine Survives Backbone Cleavage by Electron Transfer Dissociation. A Near-UV Photodissociation and Infrared Multiphoton Dissociation Action Spectroscopy Study

Overview

Journal J Am Soc Mass Spectrom

Specialty Chemistry

Date 2016 Apr 10

PMID 27059977

Citations 4

Authors

Christopher J Shaffer

Jonathan Martens

Ales Marek

Jos Oomens

Frantisek Turecek

Affiliations

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Abstract

We report a combined experimental and computational study aimed at elucidating the structure of N-terminal fragment ions of the c type produced by electron transfer dissociation of photo-leucine (L*) peptide ions GL*GGKX. The c 4 ion from GL*GGK is found to retain an intact diazirine ring that undergoes selective photodissociation at 355 nm, followed by backbone cleavage. Infrared multiphoton dissociation action spectra point to the absence in the c 4 ion of a diazoalkane group that could be produced by thermal isomerization of vibrationally hot ions. The c 4 ion from ETD of GL*GGK is assigned an amide structure by a close match of the IRMPD action spectrum and calculated IR absorption. The energetics and kinetics of c 4 ion dissociations are discussed. Graphical Abstract ᅟ.

Citing Articles

Spectroscopic Characterization of an Extensive Set of c-Type Peptide Fragment Ions Formed by Electron Transfer Dissociation Suggests Exclusive Formation of Amide Isomers.

Kempkes L, Martens J, Berden G, Oomens J J Phys Chem Lett. 2018; 9(22):6404-6411.

PMID: 30343579 PMC: 6240889. DOI: 10.1021/acs.jpclett.8b02850.

w-Type ions formed by electron transfer dissociation of Cys-containing peptides investigated by infrared ion spectroscopy.

Kempkes L, Martens J, Berden G, Oomens J J Mass Spectrom. 2018; 53(12):1207-1213.

PMID: 30281881 PMC: 6283004. DOI: 10.1002/jms.4298.

Spontaneous Isomerization of Peptide Cation Radicals Following Electron Transfer Dissociation Revealed by UV-Vis Photodissociation Action Spectroscopy.

Imaoka N, Houferak C, Murphy M, Nguyen H, Dang A, Turecek F J Am Soc Mass Spectrom. 2018; 29(9):1768-1780.

PMID: 29340957 DOI: 10.1007/s13361-017-1871-0.

Near-UV Photodissociation of Tryptic Peptide Cation Radicals. Scope and Effects of Amino Acid Residues and Radical Sites.

Nguyen H, Turecek F J Am Soc Mass Spectrom. 2017; 28(7):1333-1344.

PMID: 28155086 DOI: 10.1007/s13361-016-1586-7.

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