Protein Phosphatase 4 Catalytic Subunit is Overexpressed in Glioma and Promotes Glioma Cell Proliferation and Invasion

Overview

Journal Tumour Biol

Publisher Sage Publications

Specialty Oncology

Date 2016 Apr 10

PMID 27059736

Citations 10

Authors

Mengyou Li

Xin Li

Shiming Xu

Pengfei Xue

Qingzhe Li

Qingyang Lu

Qingbin Jia

Lianqun Zhang

Xueyuan Li

Xingang Li

Affiliations

Soon will be listed here.

Abstract

Protein phosphatase 4 catalytic subunit (PP4C) has been identified to be overexpressed in various solid cancers. However, to date, the role of PP4C in glioma remains elusive. In the present study, we aimed to detect PP4C expression in glioma patients and explore its function in glioma and prognostic significance in patients with glioma. The expression levels of PP4C mRNA and protein in 30 glioma tissue specimens and 10 non-cancerous brain tissue specimens were detected by qRT-PCR and Western blot analysis. Moreover, immunohistochemistry was performed to assess PP4C expression in 120 glioma patients. The effects of siRNA-mediated PP4C silencing on the proliferation, migration, and invasion of U251 and U87 glioma cells were assessed. We found that PP4C was upregulated in glioma tissue at both mRNA and protein levels compared with non-cancerous brain tissue. Univariate and multivariate analyses indicated that high PP4C expression was an independent prognostic factor for poor survival of glioma patients. Knockdown of PP4C reduced the proliferation, migration, and invasion of U251 and U87 cells. In conclusion, our findings suggest that PP4C plays an oncogenic role in glioma development and progression and might serve as a prognostic biomarker as well as a potential therapeutic target for glioma.

Citing Articles

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High PPP4C expression predicts poor prognosis in diffuse large B-cell lymphoma.

Hui X, Li L, Xiong W, Liu Y, Li H, Zhang H Clin Exp Med. 2024; 24(1):89.

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Protein phosphatase 4 dephosphorylates phosphofructokinase-1 to regulate its enzymatic activity.

Park J, Lee D BMB Rep. 2023; 56(11):618-623.

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SIRT1 regulates DNA damage signaling through the PP4 phosphatase complex.

Rasti G, Becker M, Vazquez B, Espinosa-Alcantud M, Fernandez-Duran I, Gamez-Garcia A Nucleic Acids Res. 2023; 51(13):6754-6769.

PMID: 37309898 PMC: 10359614. DOI: 10.1093/nar/gkad504.

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Park J, Lee D BMB Rep. 2020; 53(4):181-190.

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