Arsenic-exposed Keratinocytes Exhibit Differential MicroRNAs Expression Profile; Potential Implication of MiR-21, MiR-200a and MiR-141 in Melanoma Pathway

Overview

Journal Clin Cancer Drugs

Specialty Oncology

Date 2016 Apr 8

PMID 27054085

Citations 19

Authors

Horacio Gonzalez

Carolina Lema

Robert A Kirken

Rosa A Maldonado

Armando Varela-Ramirez

Renato J Aguilera

Affiliations

Soon will be listed here.

Abstract

Long-term exposure to arsenic has been linked to cancer in different organs and tissues, including skin. Here, non-malignant human keratinocytes (HaCaT) were exposed to arsenic and its effects on microRNAs (miRNAs; miR) expression were analyzed miRCURY LNA array analyses. A total of 30 miRNAs were found differentially expressed in arsenic-treated cells, as compared to untreated controls. Among the up-regulated miRNAs, miR-21, miR-200a and miR-141, are well known to be involved in carcinogenesis. Additional findings confirmed that those three miRNAs were indeed up-regulated in arsenic-stimulated keratinocytes as demonstrated by quantitative PCR assay. Furthermore, bioinformatics analysis of both potential cancer-related pathways and targeted genes affected by miR-21, miR-200a and/or miR-141 was performed. Results revealed that miR-21, miR-200a and miR-141 are implicated in skin carcinogenesis related with melanoma development. Conclusively, our results indicate that arsenic-treated keratinocytes exhibited alteration in the miRNAs expression profile and that miR-21, miR-200a and miR-141 could be promising early biomarkers of the epithelial phenotype of cancer cells and they could be potential novel targets for melanoma therapeutic interventions.

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