Comparison of Immunochromatographic Diagnostic Test with Hheminested Reverse Transcriptase Polymerase Chain Reaction for Detection of Rabies Virus from Brain Samples of Various Species

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2016 Apr 6

PMID 27047061

Citations 9

Authors

Pranoti Sharma

C K Singh

Deepti Narang

Affiliations

Soon will be listed here.

Abstract

Aim: Detection of rabies is a cause of serious concern in developing countries, where dearth of highly equipped laboratories and trained personnel to handle sophisticated investigations is felt. The availability of a diagnostic kit, which can be used in the field, is essential for diagnosis and control programs as well as for epidemiological surveillance of the prevalence of the disease. This study was planned to evaluate anigen rabies Ag test kit for its efficacy to be used for rapid diagnosis of rabies under field conditions. The test results were compared with hemi-nested reverse transcriptase polymerase chain reaction and with a gold standard fluorescent antibody test.

Materials And Methods: A total of 34 brain samples from different rabies suspected animals including dogs, buffaloes, cow, horse, and cat were examined in this study.

Results: Sensitivity of the kit was found to be 91.66%, specificity 100%, and accuracy was 94.11%.

Conclusion: The study implies that the immunochromatographic diagnostic test kit may be employed for diagnosis of rabies in field conditions.

Citing Articles

Evaluation of lateral flow devices for postmortem rabies diagnosis in animals in the Philippines: a multicenter study.

Cruz J, Garcia A, Saito N, Lagayan M, Pena R, Usana M J Clin Microbiol. 2023; 61(12):e0084223.

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Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals.

Kimitsuki K, Saito N, Yamada K, Park C, Inoue S, Suzuki M PLoS Negl Trop Dis. 2020; 14(11):e0008844.

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Evaluation of a rapid immunochromatographic test kit to the gold standard fluorescent antibody test for diagnosis of rabies in animals in Bhutan.

Tenzin T, Lhamo K, Rai P, Tshering D, Jamtsho P, Namgyal J BMC Vet Res. 2020; 16(1):183.

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Further Evidence of Inadequate Quality in Lateral Flow Devices Commercially Offered for the Diagnosis of Rabies.

Klein A, Fahrion A, Finke S, Eyngor M, Novak S, Yakobson B Trop Med Infect Dis. 2020; 5(1).

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Evaluation of an Immunochromatographic Assay as a Canine Rabies Surveillance Tool in Goa, India.

Yale G, Gibson A, Mani R, P K H, Costa N, Corfmat J Viruses. 2019; 11(7).

PMID: 31311178 PMC: 6669590. DOI: 10.3390/v11070649.

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