A T-RFLP Database for the Rapid Profiling of Methanogenic Communities in Anaerobic Digesters

Overview

Journal Anaerobe

Date 2016 Apr 6

PMID 27046270

Citations 10

Authors

Franziska Buhligen

Rico Lucas

Marcell Nikolausz

Sabine Kleinsteuber

Affiliations

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Abstract

We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.

Citing Articles

Effect of Inoculum Microbial Diversity in Ex Situ Biomethanation of Hydrogen.

Logrono W, Kluge P, Kleinsteuber S, Harms H, Nikolausz M Bioengineering (Basel). 2022; 9(11).

PMID: 36421079 PMC: 9687489. DOI: 10.3390/bioengineering9110678.

The next frontier of the anaerobic digestion microbiome: From ecology to process control.

De Vrieze J Environ Sci Ecotechnol. 2022; 3:100032.

PMID: 36159602 PMC: 9488066. DOI: 10.1016/j.ese.2020.100032.

Effects of combined tannic acid/fluoride on sulfur transformations and methanogenic pathways in swine manure.

Dalby F, Nikolausz M, Hansen M, Feilberg A PLoS One. 2021; 16(9):e0257759.

PMID: 34555107 PMC: 8459979. DOI: 10.1371/journal.pone.0257759.

Redox Potential Heterogeneity in Fixed-Bed Electrodes Leads to Microbial Stratification and Inhomogeneous Performance.

Quejigo J, Korth B, Kuchenbuch A, Harnisch F ChemSusChem. 2021; 14(4):1155-1165.

PMID: 33387375 PMC: 7986606. DOI: 10.1002/cssc.202002611.

Bioaugmentation of anaerobic digesters treating lignocellulosic feedstock by enriched microbial consortia.

Ozbayram E, Kleinsteuber S, Nikolausz M, Ince B, Ince O Eng Life Sci. 2020; 18(7):440-446.

PMID: 32624925 PMC: 6999511. DOI: 10.1002/elsc.201700199.