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Isolation of a Legionella Pneumophila Restriction Mutant with Increased Ability to Act As a Recipient in Heterospecific Matings

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1989 Apr 1
PMID 2703472
Citations 20
Authors
A Marra
H A Shuman
Affiliations
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Abstract

The ability of Legionella pneumophila to act as a recipient of IncP and IncQ plasmids in matings with Escherichia coli varies widely from strain to strain. We found that the low efficiency of mating of the Philadelphia-1 strain is due to a type II restriction-modification system, and we isolated and characterized a Philadelphia-1 mutant that lacks the restriction enzyme activity.

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