Mobilization of the Relaxable Staphylococcus Aureus Plasmid PC221 by the Conjugative Plasmid PGO1 Involves Three PC221 Loci

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1989 Apr 1

PMID 2703461

Citations 42

Authors

S J Projan

G L Archer

Affiliations

Soon will be listed here.

Abstract

The Staphylococcus aureus plasmid pC221, a 4.6-kilobase multicopy chloramphenicol resistance plasmid that forms plasmid-protein relaxation complexes, was mobilized for transfer by the conjugative plasmid pGO1. Two open reading frames on the pC221 genome, now designated mobA and mobB, as well as a cis-acting locus, the putative oriT, were shown to be in involved in pC221 mobilization. The mobA (but not mobB) and oriT loci were required for pC221 relaxation, and relaxation was necessary but not sufficient for pC221 mobilization by pGO1. oriT was cloned onto a pE194 derivative and complemented in trans for both relaxation and mobilization. Mobilization of relaxable plasmids in S. aureus appears to be analogous to mobilization by donation observed in gram-negative bacteria.

Citing Articles

Catabolic Ornithine Carbamoyltransferase Activity Facilitates Growth of Staphylococcus aureus in Defined Medium Lacking Glucose and Arginine.

Reslane I, Halsey C, Stastny A, Cabrera B, Ahn J, Shinde D mBio. 2022; 13(3):e0039522.

PMID: 35475645 PMC: 9239276. DOI: 10.1128/mbio.00395-22.

Glycan-Dependent Corneocyte Adherence of Staphylococcus epidermidis Mediated by the Lectin Subdomain of Aap.

Roy P, Horswill A, Fey P mBio. 2021; 12(4):e0290820.

PMID: 34253065 PMC: 8406310. DOI: 10.1128/mBio.02908-20.

Staphylococcal Plasmids, Transposable and Integrative Elements.

Firth N, Jensen S, Kwong S, Skurray R, Ramsay J Microbiol Spectr. 2018; 6(6).

PMID: 30547857 PMC: 11633639. DOI: 10.1128/microbiolspec.GPP3-0030-2018.

Mobile Genetic Elements Associated with Antimicrobial Resistance.

Partridge S, Kwong S, Firth N, Jensen S Clin Microbiol Rev. 2018; 31(4).

PMID: 30068738 PMC: 6148190. DOI: 10.1128/CMR.00088-17.

The metalloprotease SepA governs processing of accumulation-associated protein and shapes intercellular adhesive surface properties in Staphylococcus epidermidis.

Paharik A, Kotasinska M, Both A, Hoang T, Buttner H, Roy P Mol Microbiol. 2016; 103(5):860-874.

PMID: 27997732 PMC: 5480372. DOI: 10.1111/mmi.13594.

References

NOVICK R . Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology. 1967; 33(1):155-66. DOI: 10.1016/0042-6822(67)90105-5. View

Derbyshire K, Willetts N . Mobilization of the non-conjugative plasmid RSF1010: a genetic analysis of its origin of transfer. Mol Gen Genet. 1987; 206(1):154-60. DOI: 10.1007/BF00326551. View

Novick R, Brodsky R . Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus. J Mol Biol. 1972; 68(2):285-302. DOI: 10.1016/0022-2836(72)90214-8. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

NOVICK R . Plasmid-protein relaxation complexes in Staphylococcus aureus. J Bacteriol. 1976; 127(3):1177-87. PMC: 232910. DOI: 10.1128/jb.127.3.1177-1187.1976. View

Iordanescu S . Three distinct plasmids originating in the same Staphylococcus aureus strain. Arch Roum Pathol Exp Microbiol. 1976; 35(1-2):111-8. View

Iordanescu S . Temperature-sensitive mutant of a tetracycline resistance staphylococcal plasmid. Arch Roum Pathol Exp Microbiol. 1976; 35(3):257-64. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Warren G, Twigg A, Sherratt D . ColE1 plasmid mobility and relaxation complex. Nature. 1978; 274(5668):259-61. DOI: 10.1038/274259a0. View

10.

Novick R, Murphy E, Gryczan T, Baron E, Edelman I . Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps. Plasmid. 1979; 2(1):109-29. DOI: 10.1016/0147-619x(79)90010-6. View

11.

Inselburg J, Ware P . A complementation analysis of mobilization deficient mutants of the plasmid ColE1. Mol Gen Genet. 1979; 172(2):211-9. DOI: 10.1007/BF00268285. View

12.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

13.

Forbes B, Schaberg D . Transfer of resistance plasmids from Staphylococcus epidermidis to Staphylococcus aureus: evidence for conjugative exchange of resistance. J Bacteriol. 1983; 153(2):627-34. PMC: 221678. DOI: 10.1128/jb.153.2.627-634.1983. View

14.

Projan S, Carleton S, Novick R . Determination of plasmid copy number by fluorescence densitometry. Plasmid. 1983; 9(2):182-90. DOI: 10.1016/0147-619x(83)90019-7. View

15.

Archer G, Johnston J . Self-transmissible plasmids in staphylococci that encode resistance to aminoglycosides. Antimicrob Agents Chemother. 1983; 24(1):70-7. PMC: 185106. DOI: 10.1128/AAC.24.1.70. View

16.

Kreiswirth B, Lofdahl S, Betley M, OReilly M, Schlievert P, Bergdoll M . The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature. 1983; 305(5936):709-12. DOI: 10.1038/305709a0. View

17.

Novick R, Adler G, Projan S, Carleton S, Highlander S, Gruss A . Control of pT181 replication I. The pT181 copy control function acts by inhibiting the synthesis of a replication protein. EMBO J. 1984; 3(10):2399-405. PMC: 557700. DOI: 10.1002/j.1460-2075.1984.tb02146.x. View

18.

Novick R, Projan S, Kumar C, Carleton S, Gruss A, Highlander S . Replication control for pT181, an indirectly regulated plasmid. Basic Life Sci. 1985; 30:299-320. DOI: 10.1007/978-1-4613-2447-8_24. View

19.

Chan P, Ohmori H, Tomizawa J, Lebowitz J . Nucleotide sequence and gene organization of ColE1 DNA. J Biol Chem. 1985; 260(15):8925-35. View

20.

Projan S, Kornblum J, Moghazeh S, Edelman I, Gennaro M, Novick R . Comparative sequence and functional analysis of pT181 and pC221, cognate plasmid replicons from Staphylococcus aureus. Mol Gen Genet. 1985; 199(3):452-64. DOI: 10.1007/BF00330758. View