Functional Assay of Antiplatelet Drugs Based on Margination of Platelets in Flowing Blood

Overview

Journal Biointerphases

Specialties Biomedical Engineering
Biotechnology

Date 2016 Apr 1

PMID 27030476

Citations 2

Authors

Colin D Eichinger

Aaron L Fogelson

Vladimir Hlady

Affiliations

Soon will be listed here.

Abstract

A novel functional assay of antiplatelet drug efficacy was designed by utilizing the phenomena of platelet margination in flowing blood and transient platelet contacts with surface-immobilized platelet agonists. Flow margination enhances transient contacts of platelets with the walls of flow chambers covered with surface-immobilized proteins. Depending on the type and the surface density of the immobilized agonists, such transient interactions could "prime" the marginated platelet subpopulation for enhanced activation and adhesion downstream. By creating an upstream surface patch with an immobilized platelet agonist, platelet flow margination was used to test how effective antiplatelet drugs are in suppressing downstream platelet activation and adhesion. The platelet adhesion downstream was measured by a so-called "capture" patch region close to the distal end of the flow chamber. Platelet adhesion downstream was found to be dose-dependent on the upstream surface coverage of the "priming" patch, with immobilized fibrinogen acting as a platelet agonist. Several antiplatelet agents (acetylsalicylic acid, eptifibatide, and tirofiban) were evaluated for their efficacy in attenuating downstream adhesion after upstream platelet priming. The activation of the platelet population was found to be dependent on both the extent of the upstream agonist stimulus and the antiplatelet drug concentration. Such a relationship provides an opportunity to measure the efficacy of specific antiplatelet agents against the type and concentration of upstream platelet agonists.

Citing Articles

Margination of Fluorescent Polylactic Acid-Polyaspartamide based Nanoparticles in Microcapillaries In Vitro: the Effect of Hematocrit and Pressure.

Craparo E, DApolito R, Giammona G, Cavallaro G, Tomaiuolo G Molecules. 2017; 22(11).

PMID: 29143777 PMC: 6150309. DOI: 10.3390/molecules22111845.

Binary agonist surface patterns prime platelets for downstream adhesion in flowing whole blood.

Eichinger C, Hlady V Biointerphases. 2017; 12(2):02C406.

PMID: 28454486 PMC: 5409849. DOI: 10.1116/1.4982596.

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