Cholesterol Partition and Condensing Effect in Phase-Separated Ternary Mixture Lipid Multilayers

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2016 Mar 31

PMID 27028645

Citations 15

Authors

Yicong Ma

Sajal K Ghosh

David A DiLena

Sambhunath Bera

Laurence B Lurio

Atul N Parikh

Sunil K Sinha

Affiliations

Soon will be listed here.

Abstract

The cholesterol partitioning and condensing effect in the liquid-ordered (Lo) and liquid-disordered (Ld) phases were systematically investigated for ternary mixture lipid multilayers consisting of 1:1 1,2-dipalmitoyl-sn-glycero-3-phosphocholine/1,2-dioleoyl-sn-glycero-3-phosphocholine with varying concentrations of cholesterol. X-ray lamellar diffraction was used to deduce the electron density profiles of each phase. The cholesterol concentration in each phase was quantified by fitting of the electron density profiles with a newly invented basic lipid profile scaling method that minimizes the number of fitting parameters. The obtained cholesterol concentration in each phase versus total cholesterol concentration in the sample increases linearly for both phases. The condensing effect of cholesterol in ternary lipid mixtures was evaluated in terms of phosphate-to-phosphate distances, which together with the estimated cholesterol concentration in each phase was converted into an average area per molecule. In addition, the cholesterol position was determined to a precision of (±0.7Å) and an increase of disorder in the lipid packing in the Lo phase was observed for total cholesterol concentration of 20∼30%.

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References

Veatch S, Keller S . Miscibility phase diagrams of giant vesicles containing sphingomyelin. Phys Rev Lett. 2005; 94(14):148101. DOI: 10.1103/PhysRevLett.94.148101. View

Chen L, Yu Z, Quinn P . The partition of cholesterol between ordered and fluid bilayers of phosphatidylcholine: a synchrotron X-ray diffraction study. Biochim Biophys Acta. 2007; 1768(11):2873-81. DOI: 10.1016/j.bbamem.2007.07.023. View

Alsop R, Armstrong C, Maqbool A, Toppozini L, Dies H, Rheinstadter M . Cholesterol expels ibuprofen from the hydrophobic membrane core and stabilizes lamellar phases in lipid membranes containing ibuprofen. Soft Matter. 2015; 11(24):4756-67. DOI: 10.1039/c5sm00597c. View

Veatch S, Keller S . Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol. Biophys J. 2003; 85(5):3074-83. PMC: 1303584. DOI: 10.1016/S0006-3495(03)74726-2. View

Veatch S, Polozov I, Gawrisch K, Keller S . Liquid domains in vesicles investigated by NMR and fluorescence microscopy. Biophys J. 2004; 86(5):2910-22. PMC: 1304159. DOI: 10.1016/S0006-3495(04)74342-8. View

McIntosh T . The effect of cholesterol on the structure of phosphatidylcholine bilayers. Biochim Biophys Acta. 1978; 513(1):43-58. DOI: 10.1016/0005-2736(78)90110-4. View

Franks N . Structural analysis of hydrated egg lecithin and cholesterol bilayers. I. X-ray diffraction. J Mol Biol. 1976; 100(3):345-58. DOI: 10.1016/s0022-2836(76)80067-8. View

King G, White S . Determining bilayer hydrocarbon thickness from neutron diffraction measurements using strip-function models. Biophys J. 1986; 49(5):1047-54. PMC: 1329685. DOI: 10.1016/S0006-3495(86)83733-X. View

Hung W, Lee M, Chen F, Huang H . The condensing effect of cholesterol in lipid bilayers. Biophys J. 2007; 92(11):3960-7. PMC: 1868968. DOI: 10.1529/biophysj.106.099234. View

10.

Veatch S, Soubias O, Keller S, Gawrisch K . Critical fluctuations in domain-forming lipid mixtures. Proc Natl Acad Sci U S A. 2007; 104(45):17650-5. PMC: 2077022. DOI: 10.1073/pnas.0703513104. View

11.

Kucerka N, Tristram-Nagle S, Nagle J . Closer look at structure of fully hydrated fluid phase DPPC bilayers. Biophys J. 2006; 90(11):L83-5. PMC: 1459514. DOI: 10.1529/biophysj.106.086017. View

12.

Blaurock A . Structure of the nerve myelin membrane: proof of the low-resolution profile. J Mol Biol. 1971; 56(1):35-52. DOI: 10.1016/0022-2836(71)90082-9. View

13.

Pabst G, Kucerka N, Nieh M, Rheinstadter M, Katsaras J . Applications of neutron and X-ray scattering to the study of biologically relevant model membranes. Chem Phys Lipids. 2010; 163(6):460-79. DOI: 10.1016/j.chemphyslip.2010.03.010. View

14.

Mills T, Huang J, Feigenson G, Nagle J . Effects of cholesterol and unsaturated DOPC lipid on chain packing of saturated gel-phase DPPC bilayers. Gen Physiol Biophys. 2009; 28(2):126-39. PMC: 2731993. DOI: 10.4149/gpb_2009_02_126. View

15.

Ghosh S, Aeffner S, Salditt T . Effect of PIP2 on bilayer structure and phase behavior of DOPC: an X-ray scattering study. Chemphyschem. 2011; 12(14):2633-40. DOI: 10.1002/cphc.201100154. View

16.

Greenwood A, Tristram-Nagle S, Nagle J . Partial molecular volumes of lipids and cholesterol. Chem Phys Lipids. 2006; 143(1-2):1-10. PMC: 2695672. DOI: 10.1016/j.chemphyslip.2006.04.002. View

17.

Levine Y, Wilkins M . Structure of oriented lipid bilayers. Nat New Biol. 1971; 230(11):69-72. DOI: 10.1038/newbio230069a0. View

18.

Brown D, Rose J . Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell. 1992; 68(3):533-44. DOI: 10.1016/0092-8674(92)90189-j. View

19.

Armstrong C, Marquardt D, Dies H, Kucerka N, Yamani Z, Harroun T . The Observation of Highly Ordered Domains in Membranes with Cholesterol. PLoS One. 2013; 8(6):e66162. PMC: 3688844. DOI: 10.1371/journal.pone.0066162. View

20.

Kucerka N, Perlmutter J, Pan J, Tristram-Nagle S, Katsaras J, Sachs J . The effect of cholesterol on short- and long-chain monounsaturated lipid bilayers as determined by molecular dynamics simulations and X-ray scattering. Biophys J. 2008; 95(6):2792-805. PMC: 2527263. DOI: 10.1529/biophysj.107.122465. View