A Developmentally Regulated Switch from Stem Cells to Dedifferentiation for Limb Muscle Regeneration in Newts

Overview

Journal Nat Commun

Specialty Biology

Date 2016 Mar 31

PMID 27026263

Citations 49

Authors

Hibiki Vincent Tanaka

Nathaniel Chuen Yin Ng

Zhan Yang Yu

Martin Miguel Casco-Robles

Fumiaki Maruo

Panagiotis A Tsonis

Chikafumi Chiba

Affiliations

Soon will be listed here.

Abstract

The newt, a urodele amphibian, is able to repeatedly regenerate its limbs throughout its lifespan, whereas other amphibians deteriorate or lose their ability to regenerate limbs after metamorphosis. It remains to be determined whether such an exceptional ability of the newt is either attributed to a strategy, which controls regeneration in larvae, or on a novel one invented by the newt after metamorphosis. Here we report that the newt switches the cellular mechanism for limb regeneration from a stem/progenitor-based mechanism (larval mode) to a dedifferentiation-based one (adult mode) as it transits beyond metamorphosis. We demonstrate that larval newts use stem/progenitor cells such as satellite cells for new muscle in a regenerated limb, whereas metamorphosed newts recruit muscle fibre cells in the stump for the same purpose. We conclude that the newt has evolved novel strategies to secure its regenerative ability of the limbs after metamorphosis.

Citing Articles

One-step Cre-loxP organism creation by TAx9.

Casco-Robles M, Echigoya T, Shimazaki T, Murakami Y, Hirano M, Maruo F Commun Biol. 2025; 8(1):340.

PMID: 40050380 PMC: 11885649. DOI: 10.1038/s42003-025-07759-9.

Sustained induction of autophagy enhances survival during prolonged starvation in newt cells.

Hasan M, Goto S, Sekiya R, Hayashi T, Li T, Kawabata T Life Sci Alliance. 2025; 8(4).

PMID: 39904566 PMC: 11794943. DOI: 10.26508/lsa.202402772.

An adult myogenic cell line of the Japanese fire-bellied newt Cynops pyrrhogaster.

Shiga S, Murakami Y, Wang Z, Ando R, Casco-Robles M, Maruo F Sci Rep. 2024; 14(1):30041.

PMID: 39627485 PMC: 11614899. DOI: 10.1038/s41598-024-81899-6.

Development of a ZRS Reporter System for the Newt () During Terrestrial Limb Regeneration.

Casco-Robles M, Ikeda R, Maruo F, Chiba C Biomedicines. 2024; 12(11).

PMID: 39595071 PMC: 11591917. DOI: 10.3390/biomedicines12112505.

Hallmarks of regeneration.

Poss K, Tanaka E Cell Stem Cell. 2024; 31(9):1244-1261.

PMID: 39163854 PMC: 11410156. DOI: 10.1016/j.stem.2024.07.007.

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