Protective Effects of Honokiol on Ischemia/reperfusion Injury of Rat Ovary: an Experimental Study

Overview

Journal Drug Des Devel Ther

Specialty Pharmacology

Date 2016 Mar 30

PMID 27022246

Citations 5

Authors

Senem Yaman Tunc

Elif Agacayak

Neval Yaman Goruk

Mehmet Sait Icen

Abdulkadir Turgut

Ulas Alabalik

Cihan Togrul

Cenap Ekinci

Aysun Ekinci

Talip Gul

Affiliations

Soon will be listed here.

Abstract

Aim: The purpose of this study was to investigate the protective effect of honokiol on experimental ischemia/reperfusion injury of rat ovary.

Materials And Methods: A total of 40 female Wistar albino rats were used in this study. The rats were divided into five groups as follows: sham (Group I), torsion (Group II), torsion + detorsion (Group III), torsion + detorsion + saline (Group IV), and torsion + detorsion + honokiol (Group V). Bilateral adnexa in all the rats except for those in the sham group were exposed to torsion for 3 hours. The rats in Group IV were administered saline, whereas the rats in Group V were administered honokiol by intraperitoneal route 30 minutes before detorsion. Tissue and plasma concentrations of malondialdehyde and nitric oxide were determined. Ovarian tissue was histologically evaluated. Data analyses were performed by means of Kruskal-Wallis test and Mann-Whitney U-test (Bonferroni correction) in SPSS 15.0 (Statistical Package for Social Sciences; SPSS Inc., Chicago, IL, USA).

Results: The torsion and detorsion groups had higher scores in vascular congestion, hemorrhage, and inflammatory cell infiltration compared with the sham group (P<0.005). In addition, total histopathological scores were significantly higher in the torsion and detorsion groups compared with the sham group (P<0.005). A significant reduction was observed in hemorrhage, inflammatory cell infiltration, and cellular degeneration scores, of all histopathological scores, in the honokiol group (P<0.005). Ovarian tissue concentrations of malondialdehyde were significantly higher in the torsion and detorsion groups compared with the sham and honokiol groups (P<0.005). Ovarian tissue concentrations of nitric oxide, on the other hand, were significantly higher in the torsion group compared with the sham, saline, and honokiol groups (P<0.005).

Conclusion: Honokiol has a beneficial effect on ovarian torsion-related ischemia/reperfusion injury.

Citing Articles

Neuropharmacological potential of honokiol and its derivatives from Chinese herb Magnolia species: understandings from therapeutic viewpoint.

Faysal M, Khan J, Zehravi M, Nath N, Singh L, Kakkar S Chin Med. 2023; 18(1):154.

PMID: 38001538 PMC: 10668527. DOI: 10.1186/s13020-023-00846-1.

Honokiol Provides Cardioprotection from Myocardial Ischemia/Reperfusion Injury (MI/RI) by Inhibiting Mitochondrial Apoptosis via the PI3K/AKT Signaling Pathway.

Lv L, Kong Q, Li Z, Zhang Y, Chen B, Lv L Cardiovasc Ther. 2022; 2022:1001692.

PMID: 35414825 PMC: 8977331. DOI: 10.1155/2022/1001692.

Honokiol Ameliorates Myocardial Ischemia/Reperfusion Injury in Type 1 Diabetic Rats by Reducing Oxidative Stress and Apoptosis through Activating the SIRT1-Nrf2 Signaling Pathway.

Zhang B, Zhai M, Li B, Liu Z, Li K, Jiang L Oxid Med Cell Longev. 2018; 2018:3159801.

PMID: 29675132 PMC: 5838504. DOI: 10.1155/2018/3159801.

Magnesium: does it reduce ischemia/reperfusion injury in an adnexal torsion rat model?.

Celik Kavak E, Gulcu Bulmus F, Bulmus O, Kavak S, Kocaman N Drug Des Devel Ther. 2018; 12:409-415.

PMID: 29535502 PMC: 5836654. DOI: 10.2147/DDDT.S157115.

The Protective Effects of Alpha-Lipoic Acid and Coenzyme Q10 Combination on Ovarian Ischemia-Reperfusion Injury: An Experimental Study.

Tuncer A, Bozkurt M, Koken T, Dogan N, Pektas M, Embleton D Adv Med. 2016; 2016:3415046.

PMID: 27597986 PMC: 5002467. DOI: 10.1155/2016/3415046.

References

Cortas N, Wakid N . Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem. 1990; 36(8 Pt 1):1440-3. View

Gutteridge J . Free radicals in disease processes: a compilation of cause and consequence. Free Radic Res Commun. 1993; 19(3):141-58. DOI: 10.3109/10715769309111598. View

Akgur F, Kilinc K, Aktug T . Reperfusion injury after detorsion of unilateral testicular torsion. Urol Res. 1993; 21(6):395-9. DOI: 10.1007/BF00300075. View

Kuroda S, Siesjo B . Reperfusion damage following focal ischemia: pathophysiology and therapeutic windows. Clin Neurosci. 1997; 4(4):199-212. View

Hayashi T, Saito A, Okuno S, Ferrand-Drake M, Dodd R, Chan P . Damage to the endoplasmic reticulum and activation of apoptotic machinery by oxidative stress in ischemic neurons. J Cereb Blood Flow Metab. 2005; 25(1):41-53. DOI: 10.1038/sj.jcbfm.9600005. View

Land W . The role of postischemic reperfusion injury and other nonantigen-dependent inflammatory pathways in transplantation. Transplantation. 2005; 79(5):505-14. DOI: 10.1097/01.tp.0000153160.82975.86. View

Del Rio D, Stewart A, Pellegrini N . A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis. 2005; 15(4):316-28. DOI: 10.1016/j.numecd.2005.05.003. View

Tse A, Wan C, Shen X, Yang M, Fong W . Honokiol inhibits TNF-alpha-stimulated NF-kappaB activation and NF-kappaB-regulated gene expression through suppression of IKK activation. Biochem Pharmacol. 2005; 70(10):1443-57. DOI: 10.1016/j.bcp.2005.08.011. View

Oelsner G, Shashar D . Adnexal torsion. Clin Obstet Gynecol. 2006; 49(3):459-63. DOI: 10.1097/00003081-200609000-00006. View

10.

Sheu M, Liu S, Lan K . Honokiol induces calpain-mediated glucose-regulated protein-94 cleavage and apoptosis in human gastric cancer cells and reduces tumor growth. PLoS One. 2007; 2(10):e1096. PMC: 2034534. DOI: 10.1371/journal.pone.0001096. View

11.

Somuncu S, Cakmak M, Dikmen G, Akman H, Kaya M . Ischemia-reperfusion injury of rabbit ovary and protective effect of trapidil: an experimental study. Pediatr Surg Int. 2007; 24(3):315-8. DOI: 10.1007/s00383-007-2079-3. View

12.

McWilliams G, Hill M, Dietrich 3rd C . Gynecologic emergencies. Surg Clin North Am. 2008; 88(2):265-83, vi. DOI: 10.1016/j.suc.2007.12.007. View

13.

Lubos E, Handy D, Loscalzo J . Role of oxidative stress and nitric oxide in atherothrombosis. Front Biosci. 2008; 13:5323-44. PMC: 2617738. DOI: 10.2741/3084. View

14.

Dikalov S, Losik T, Arbiser J . Honokiol is a potent scavenger of superoxide and peroxyl radicals. Biochem Pharmacol. 2008; 76(5):589-96. PMC: 2575413. DOI: 10.1016/j.bcp.2008.06.012. View

15.

Kir Sahin F, Cosar E, Koken G, Toy H, Basarali K, Buyukbas S . Protective effect of aprotinin on ischemia-reperfusion injury in rat ovary. J Obstet Gynaecol Res. 2008; 34(5):794-800. DOI: 10.1111/j.1447-0756.2008.00846.x. View

16.

Lee S, Cho J . Inhibitory effects of honokiol on LPS and PMA-induced cellular responses of macrophages and monocytes. BMB Rep. 2009; 42(9):574-9. DOI: 10.5483/bmbrep.2009.42.9.574. View

17.

Zhang G, Wang R, Zhang H, Zhang G, Luo M, Luo J . Effects of chronic treatment with honokiol in spontaneously hypertensive rats. Biol Pharm Bull. 2010; 33(3):427-31. DOI: 10.1248/bpb.33.427. View

18.

Shen J, Man K, Huang P, Chen W, Chen D, Cheng Y . Honokiol and magnolol as multifunctional antioxidative molecules for dermatologic disorders. Molecules. 2010; 15(9):6452-65. PMC: 6257695. DOI: 10.3390/molecules15096452. View

19.

Ergun Y, Koc A, Dolapcioglu K, Akaydin Y, Dogruer G, Kontas T . The protective effect of erythropoietin and dimethylsulfoxide on ischemia-reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol. 2010; 152(2):186-90. DOI: 10.1016/j.ejogrb.2010.05.025. View

20.

Huang K, Weng T, Huang H, Huang K, Lin W, Chen S . Honokiol attenuates torsion/detorsion-induced testicular injury in rat testis by way of suppressing endoplasmic reticulum stress-related apoptosis. Urology. 2012; 79(4):967.e5-11. DOI: 10.1016/j.urology.2011.11.027. View