The IncP-6 Plasmid P10265-KPC from Pseudomonas Aeruginosa Carries a Novel ΔISEc33-Associated Bla KPC-2 Gene Cluster

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2016 Mar 26

PMID 27014233

Citations 35

Authors

Xiaotian Dai

Dongsheng Zhou

Wei Xiong

Jiao Feng

Wenbo Luo

Guangming Luo

Haijing Wang

Fengjun Sun

Xiangdong Zhou

Affiliations

Soon will be listed here.

Abstract

Pseudomonas aeruginosa strain 10265 was recovered from a patient with pneumonia in a Chinese public hospital, and it displays the carbapenem resistance phenotype due to the acquisition of a non-conjugative but mobilizable IncP-6-type plasmid p10265-KPC. p10265-KPC carries a Tn5563-borne defective mer locus, and a novel ΔISEc33-associated bla KPC-2 gene cluster without paired inverted repeats and paired direct repeats at both ends. Mobilization of this ΔISEc33-associated element in p10265-KPC would be attributed to homologous recombination-based insertion of a foreign structure Tn3-ISApu1-orf7-ISApu2- ISKpn27-Δbla TEM-1 -bla KPC-2 -ΔISKpn6- korC-orf6-klcA-ΔrepB into a pre-existent intact ISEc33, making ISEc33 truncated at the 3' end. The previously reported pCOL-1 represents the first sequenced KPC-producing IncP-6 plasmid, while p10265-KPC is the second one. These two plasmids carry two distinct bla KPC-2 gene clusters, which are inserted into the different sites of the IncP-6 backbone and have different evolutionary histories of assembly and mobilization. This is the first report of identification of the IncP-6-type resistance plasmid in China.

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