Laboratory Detection and Clinical Implication of Oxacillinase-48 Like Carbapenemase: The Hidden Threat

Overview

Journal J Glob Infect Dis

Date 2016 Mar 26

PMID 27013843

Citations 44

Authors

Yamuna Devi Bakthavatchalam

Shalini Anandan

Balaji Veeraraghavan

Affiliations

Soon will be listed here.

Abstract

Carbapenemase producing Gram-negative pathogen is of great concern for physician. The challenging aspects are treatment option and infection control. Monitoring of respective carbapenemase resistance mechanism is necessary to prevent the outbreaks. Currently, the rapid emergence of oxacillinase (OXA-48) like is alarming. Increasing frequency of OXA-48 is seen than the classical carbapenemase (KPC, NDM, IMP, and VIM) across the world. The bla OXA-48 gene is commonly identified in Escherichia coli and Klebsiella pneumoniae. The transferrable plasmid of OXA-48 is associated with rapid spread and inter-species dissemination. In general, OXA-48-like enzymes weakly hydrolyzes both carbapenem and broad spectrum cephalosporins. Except OXA-163, which effectively hydrolyze cephalosporin. This poor hydrolytic profile obscures the detection of OXA-48-like. It may go undetected in routine diagnosis and complicates the treatment option. Co-production of OXA-48-like with CTX-M-15 and other carbapenemase (NDM, VIM) leads to the emergence of multidrug resistant strains.

Citing Articles

Phenotypic and molecular methods of carbapenemase detection: Can we break the chain and preserve the carbapenems?.

Kumar M, Bhalla G, Tandel K, Jain S, Singh G, Mahajan P Med J Armed Forces India. 2025; 80(6):657-666.

PMID: 39990540 PMC: 11842927. DOI: 10.1016/j.mjafi.2023.05.008.

Emergence of population heterogeneity in Klebsiella pneumoniae with a bla-harboring plasmid: carbapenem resistance, virulence, and fitness.

Cho Y, Kim S, Ko K J Biomed Sci. 2025; 32(1):22.

PMID: 39953534 PMC: 11829361. DOI: 10.1186/s12929-024-01108-4.

Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative Bacteria.

Savelieva J, Kondratieva D, Golikova M Biomedicines. 2025; 13(1).

PMID: 39857821 PMC: 11760439. DOI: 10.3390/biomedicines13010238.

Performance of Ertapenem-Supplemented MacConkey Agar (MacErt) for Detecting Carbapenemase-Producing Enterobacterales.

Sow O, Cisse A, Ndiaye I, Niang E, Kane F, Cisse K Cureus. 2024; 16(11):e74106.

PMID: 39712805 PMC: 11661862. DOI: 10.7759/cureus.74106.

Epidemiological and genetic characteristics of clinical carbapenemase-producing Enterobacterales isolates from Batna hospitals in Algeria.

Benbouza A, Kassah-Laouar A, Chelaghma W, Bouziane F, Mebarki Y, Rolain J BMC Infect Dis. 2024; 24(1):1439.

PMID: 39695434 PMC: 11654383. DOI: 10.1186/s12879-024-10300-8.

References

Shibl A, Al-Agamy M, Memish Z, Senok A, Khader S, Assiri A . The emergence of OXA-48- and NDM-1-positive Klebsiella pneumoniae in Riyadh, Saudi Arabia. Int J Infect Dis. 2013; 17(12):e1130-3. DOI: 10.1016/j.ijid.2013.06.016. View

Gulmez D, Woodford N, Palepou M, Mushtaq S, Metan G, Yakupogullari Y . Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae isolates from Turkey with OXA-48-like carbapenemases and outer membrane protein loss. Int J Antimicrob Agents. 2008; 31(6):523-6. DOI: 10.1016/j.ijantimicag.2008.01.017. View

Giani T, Conte V, Di Pilato V, Aschbacher R, Weber C, Larcher C . Escherichia coli from Italy producing OXA-48 carbapenemase encoded by a novel Tn1999 transposon derivative. Antimicrob Agents Chemother. 2012; 56(4):2211-3. PMC: 3318333. DOI: 10.1128/AAC.00035-12. View

Tijet N, Boyd D, Patel S, Mulvey M, Melano R . Evaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2013; 57(9):4578-80. PMC: 3754310. DOI: 10.1128/AAC.00878-13. View

Abdelaziz M, Bonura C, Aleo A, El-Domany R, Fasciana T, Mammina C . OXA-163-producing Klebsiella pneumoniae in Cairo, Egypt, in 2009 and 2010. J Clin Microbiol. 2012; 50(7):2489-91. PMC: 3405599. DOI: 10.1128/JCM.06710-11. View

Potron A, Nordmann P, Lafeuille E, Maskari Z, Al Rashdi F, Poirel L . Characterization of OXA-181, a carbapenem-hydrolyzing class D beta-lactamase from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2011; 55(10):4896-9. PMC: 3186949. DOI: 10.1128/AAC.00481-11. View

Girlich D, Poirel L, Nordmann P . Value of the modified Hodge test for detection of emerging carbapenemases in Enterobacteriaceae. J Clin Microbiol. 2011; 50(2):477-9. PMC: 3264163. DOI: 10.1128/JCM.05247-11. View

Berger S, Alauzet C, Aissa N, Henard S, Rabaud C, Bonnet R . Characterization of a new blaOXA-48-carrying plasmid in Enterobacteriaceae. Antimicrob Agents Chemother. 2013; 57(8):4064-7. PMC: 3719702. DOI: 10.1128/AAC.02550-12. View

Dortet L, Poirel L, Al Yaqoubi F, Nordmann P . NDM-1, OXA-48 and OXA-181 carbapenemase-producing Enterobacteriaceae in Sultanate of Oman. Clin Microbiol Infect. 2012; 18(5):E144-8. DOI: 10.1111/j.1469-0691.2012.03796.x. View

10.

Temkin E, Adler A, Lerner A, Carmeli Y . Carbapenem-resistant Enterobacteriaceae: biology, epidemiology, and management. Ann N Y Acad Sci. 2014; 1323:22-42. DOI: 10.1111/nyas.12537. View

11.

Cuzon G, Ouanich J, Gondret R, Naas T, Nordmann P . Outbreak of OXA-48-positive carbapenem-resistant Klebsiella pneumoniae isolates in France. Antimicrob Agents Chemother. 2011; 55(5):2420-3. PMC: 3088266. DOI: 10.1128/AAC.01452-10. View

12.

Levast M, Poirel L, Carrer A, Deiber M, Decroisette E, Mallaval F . Transfer of OXA-48-positive carbapenem-resistant Klebsiella pneumoniae from Turkey to France. J Antimicrob Chemother. 2011; 66(4):944-5. DOI: 10.1093/jac/dkq504. View

13.

Goren M, Chmelnitsky I, Carmeli Y, Navon-Venezia S . Plasmid-encoded OXA-48 carbapenemase in Escherichia coli from Israel. J Antimicrob Chemother. 2010; 66(3):672-3. DOI: 10.1093/jac/dkq467. View

14.

Mimoz O, Gregoire N, Poirel L, Marliat M, Couet W, Nordmann P . Broad-spectrum β-lactam antibiotics for treating experimental peritonitis in mice due to Klebsiella pneumoniae producing the carbapenemase OXA-48. Antimicrob Agents Chemother. 2012; 56(5):2759-60. PMC: 3346608. DOI: 10.1128/AAC.06069-11. View

15.

Castanheira M, Deshpande L, Mathai D, Bell J, Jones R, Mendes R . Early dissemination of NDM-1- and OXA-181-producing Enterobacteriaceae in Indian hospitals: report from the SENTRY Antimicrobial Surveillance Program, 2006-2007. Antimicrob Agents Chemother. 2010; 55(3):1274-8. PMC: 3067112. DOI: 10.1128/AAC.01497-10. View

16.

Cuzon G, Naas T, Lesenne A, Benhamou M, Nordmann P . Plasmid-mediated carbapenem-hydrolysing OXA-48 beta-lactamase in Klebsiella pneumoniae from Tunisia. Int J Antimicrob Agents. 2010; 36(1):91-3. DOI: 10.1016/j.ijantimicag.2010.02.014. View

17.

Poirel L, Naas T, Nordmann P . Diversity, epidemiology, and genetics of class D beta-lactamases. Antimicrob Agents Chemother. 2009; 54(1):24-38. PMC: 2798486. DOI: 10.1128/AAC.01512-08. View

18.

Khajuria A, Praharaj A, Kumar M, Grover N . Emergence of Escherichia coli, Co-Producing NDM-1 and OXA-48 Carbapenemases, in Urinary Isolates, at a Tertiary Care Centre at Central India. J Clin Diagn Res. 2014; 8(6):DC01-4. PMC: 4129265. DOI: 10.7860/JCDR/2014/7952.4413. View

19.

Glupczynski Y, Huang T, Bouchahrouf W, Rezende de Castro R, Bauraing C, Gerard M . Rapid emergence and spread of OXA-48-producing carbapenem-resistant Enterobacteriaceae isolates in Belgian hospitals. Int J Antimicrob Agents. 2011; 39(2):168-72. DOI: 10.1016/j.ijantimicag.2011.10.005. View

20.

Dortet L, Poirel L, Nordmann P . Rapid identification of carbapenemase types in Enterobacteriaceae and Pseudomonas spp. by using a biochemical test. Antimicrob Agents Chemother. 2012; 56(12):6437-40. PMC: 3497194. DOI: 10.1128/AAC.01395-12. View