Comparison of Ultrafast Papanicolaou Stain with the Standard Papanicolaou Stain in Body Fluids and Fine Needle Aspiration Specimens

Overview

Journal J Lab Physicians

Specialty Pathology

Date 2016 Mar 26

PMID 27013808

Citations 3

Authors

Nasar Yousuf Alwahaibi

Mariam Said Alsubhi

Najat Aldairi

Amna Alshukaili

Usha Rani Bai

Affiliations

Soon will be listed here.

Abstract

Introduction: Most cytology laboratories in all Gulf countries including Oman, use the standard papanicolaou (PAP) method to stain various cytological specimens. The aim of this study was to investigate the possible application of ultrafast PAP (UF-PAP) method in cytology laboratory.

Materials And Methods: Samples from 46 patients containing 26 body fluids and 20 fine needle aspirations (FNAs) (9 thyroids and 11 breasts) were collected. Two air dried and two wet smears from each sample were prepared and stained by UF-PAP and the standard PAP stains, respectively. Background, nuclear staining, cell morphology, and overall staining were independently reviewed by two cytoscreeners.

Results: In all cases of FNA, UF-PAP stain gave a good score for the background, nuclear staining, cell morphology, and overall staining when compared with the standard PAP method. Although the correct diagnosis was made in all cases of body cavity fluids cases except in one case, UF-PAP stain gave a fewer score in the assessment of body cavity fluid samples.

Conclusion: The findings of this study support the use of UF-PAP method in cytology laboratory with a high emphasis on FNA samples.

Citing Articles

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Modified Ultrafast Papanicolaou Stain in Ultrasound Guided FNAC of Intra-abdominal Lesions.

Kamalkant Shastri S, Joshi A Iran J Pathol. 2020; 15(2):66-74.

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Comparative Assessment of Conventional Papanicolaou and Modified Ultrafast Papanicolaou Stains in Fine Needle Aspiration Samples and Body Fluids.

Arul P, Eniya S, Pushparaj M, Masilamani S, Kanmani P, Lingasamy C J Cytol. 2018; 35(1):46-50.

PMID: 29403170 PMC: 5795728. DOI: 10.4103/JOC.JOC_94_17.

References

Yang G, Alvarez I . Ultrafast Papanicolaou stain. An alternative preparation for fine needle aspiration cytology. Acta Cytol. 1995; 39(1):55-60. View

Kamal M, Kulkarni M, Wahane R . Ultrafast papanicolaou stain modified for developing countries: efficacy and pitfalls. Acta Cytol. 2011; 55(2):205-12. DOI: 10.1159/000320909. View

Yang G, Liebeskind D, Messina A . Diagnostic accuracy of follicular variant of papillary thyroid carcinoma in fine-needle aspirates processed by ultrafast Papanicolaou stain: histologic follow-up of 125 cases. Cancer. 2006; 108(3):174-9. DOI: 10.1002/cncr.21837. View

Choudhary P, Sudhamani S, Pandit A, Kiri V . Comparison of modified ultrafast Papanicolaou stain with the standard rapid Papanicolaou stain in cytology of various organs. J Cytol. 2013; 29(4):241-5. PMC: 3543592. DOI: 10.4103/0970-9371.103942. View

Miller A, Chamberlain J, Day N, Hakama M, Prorok P . Report on a Workshop of the UICC Project on Evaluation of Screening for Cancer. Int J Cancer. 1990; 46(5):761-9. DOI: 10.1002/ijc.2910460502. View

Yang G . Clear nuclei are specific to papillary carcinoma in thyroid fine-needle aspirates processed by Ultrafast Papanicolaou stain. Diagn Cytopathol. 2003; 29(4):236-7. DOI: 10.1002/dc.10350. View

Sato M, Taniguchi E, Kagiya T, Nunobiki O, Yang Q, Nakamura M . A modified rapid Papanicolaou stain for imprint smears. Acta Cytol. 2004; 48(3):461-2. View

Lemos L, Baliga M, Cason Z . Ultrafast Papanicolaou stain: one year's experience in a fine needle aspiration service. Acta Cytol. 1997; 41(5):1630-1. DOI: 10.1159/000332872. View

Kamal M, Bodele A, Munshi M, Bobhate S, Kher A . Efficacy of a modified Ultra Fast Papanicolaou (UFP) stain for breast aspirates. Indian J Pathol Microbiol. 2001; 43(4):417-21. View

10.

Yang G, Liebeskind D, Messina A . Ultrasound-guided fine-needle aspiration of the thyroid assessed by Ultrafast Papanicolaou stain: data from 1135 biopsies with a two- to six-year follow-up. Thyroid. 2001; 11(6):581-9. DOI: 10.1089/105072501750302895. View

11.

Maruta J, Hashimoto H, Yamashita H, Yamashita H, Noguchi S . Quick aspiration cytology for thyroid nodules by modified Ultrafast Papanicolaou staining. Diagn Cytopathol. 2003; 28(1):45-8. DOI: 10.1002/dc.19999. View

12.

Einstein A, Yang G, Silberfarb J, Gil J . Effect of ultrafast Papanicolaou staining on nuclear and textural features in breast cancer cytology. Anal Quant Cytol Histol. 1997; 19(4):361-7. View

13.

Bando K, Haba R, Kushida Y, Kadota K, Katsuki N, Ohmichi K . Utility of immediate cytologic diagnosis of lung masses using ultrafast Papanicolaou stain. Lung Cancer. 2010; 72(2):172-6. DOI: 10.1016/j.lungcan.2010.08.019. View

14.

Shinde P, Pandit A . Application of modified ultrafast Papanicolaou stain in cytology of various organs. Diagn Cytopathol. 2006; 34(2):135-9. DOI: 10.1002/dc.20360. View

15.

Dighe S, Ajit D, Pathuthara S, Chinoy R . Papanicolaou stain: Is it economical to switch to rapid, economical, acetic acid, papanicolaou stain?. Acta Cytol. 2006; 50(6):643-6. DOI: 10.1159/000326034. View

16.

Gupta S, Chachra K, Bhadola P, Sodhani P . Modified Papanicolaou staining protocol with minimum alcohol use: a cost-cutting measure for resource-limited settings. Cytopathology. 2009; 21(4):229-33. DOI: 10.1111/j.1365-2303.2009.00699.x. View

17.

Bandlish U, Kapoor K, Shukla S . Efficacy of a modified ultrafast Papanicolaou (UFP) stain for breast aspirates. J Indian Med Assoc. 2005; 102(6):309, 312, 326. View

18.

Yang G, Papellas J, Wu H, Waisman J . Application of Ultrafast Papanicolaou stain to body fluid cytology. Acta Cytol. 2001; 45(2):180-5. DOI: 10.1159/000327274. View