Association of MiR-34a-3p/5p, MiR-141-3p/5p, and MiR-24 in Decidual Natural Killer Cells with Unexplained Recurrent Spontaneous Abortion

Overview

Journal Med Sci Monit

Specialties General Medicine
Pathology

Date 2016 Mar 22

PMID 26996957

Citations 27

Authors

Dandan Li

Jian Li

Affiliations

Soon will be listed here.

Abstract

Background: The specific causes of recurrent spontaneous abortion (RSA) remain unknown in 37-79% of affected women. The aim of this study was to explore the expression levels of 6 miRNAs in natural killer (NK) cells from the decidua of patients with unexplained RSA (URSA) and to predict the target genes of 3 miRNAs.

Material/methods: Two groups were examined: URSA (n=20) and controls (n=20). Flow cytometry analysis was used to identify NK cells isolated from the decidua. Transcriptional levels of miRNA were monitored using quantitative real-time reverse transcription-polymerase chain reaction. Prediction and analysis of mRNA targets of differentially expressed miRNAs were performed using bioinformatics methods.

Results: Five miRNAs [miR-34a (+281%, P<0.001), miR-155 (+396%, P<0.001), miR-141 (+142%, P<0.01), miR-125a (+279%, P<0.001), and miR-125b (+185%, P<0.001)] were up-regulated, while miR-24 was down-regulated (-64%, P<0.01) in the URSA group, compared to the control group. This study identified potential miRNA targets: miR-34a-3p/5p, 585/1718 (targets of miR-34a-3p/targets of miR-34a-5p), miR-141-3p/5p, 2270/629 (targets of miR-141-3p/targets of miR-141-5p), and miR-24, 2320 target genes. A total of 140 pathways related to target genes were identified including PI3K-Akt, focal adhesion, MAPK, Wnt, regulation of the actin cytoskeleton, T cell receptor, TGF-β, and estrogen signaling pathways.

Conclusions: This study suggests that miR-34a-3p/5p, miR-141-3p/5p, and miR-24 in decidual NK cells could be associated with URSA. These findings might contribute to the panel of diagnostic and prognostic biomarkers with clinical utility, and facilitate the development of new strategies for targeted therapy against URSA.

Citing Articles

Exploring the role of endoplasmic reticulum stress in recurrent spontaneous abortion: Identification of diagnostic biomarkers and immune cell interactions.

Tang T, Fu J, Zhang C, Wang X, Cao H, Chen L Heliyon. 2024; 10(19):e38964.

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The Expression of microRNAs and Their Involvement in Recurrent Pregnancy Loss.

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Che X, Wang X, Wang L, Xu L, Zou L, Ma T Clinics (Sao Paulo). 2024; 79:100327.

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Bioinformatics analysis of competing endogenous RNA network in decidual natural killer cell from unexplained recurrent spontaneous abortion.

Han D, Jia N Medicine (Baltimore). 2023; 102(41):e35078.

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