Combined Antenatal Therapy with Retinoic Acid and Tracheal Occlusion in a Rat Model of Congenital Diaphragmatic Hernia

Overview

Journal Pediatr Surg Int

Specialties General Surgery
Pediatrics

Date 2016 Mar 20

PMID 26992387

Citations 4

Authors

Augusto Frederico Schmidt

Frances Lilian Lanhellas Goncalves

Rebeca Lopes Figueira

Federico Scorletti

Jose Luis Peiro

Lourenco Sbragia

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the effect of combined prenatal treatment with retinoic acid (RA) and tracheal occlusion (TO) on the pulmonary vascular morphology and expression of vascular endothelial growth factors (VEGF) and its receptors in a rat model of congenital diaphragmatic hernia (CDH).

Material And Methods: Rats were given nitrofen at 9 days of gestation followed by no treatment (CDH), RA (CDH + RA), TO (CDH + TO), or both (CDH + RA + TO) (n = 16). We measured the median wall thickness of the pulmonary arterioles (MWT) and analyzed the expression of VEGF and its receptors (VEGFR1 and VEGFR2).

Results: Compared to control animals, CDH had increased MWT (44 ± 15 vs. 58 ± 7; p < 0.05) and decreased expression of VEGF, VEGFR1, and VEGFR2 (p < 0.05). Treatment with RA or TO alone, and RA + TO reduced the MWT (46 ± 9, 42 ± 11, 46 ± 8, respectively) and improved the expression of VEGF, VEGFR1, and VEGFR2 compared to CDH (p < 0.05). However, the combination of RA + TO did not confer additional benefit in the reduction of the MWT or in increasing the VEGF and its receptors compared to either treatment alone.

Conclusion: Antenatal treatment with either RA or TO improved the MWT and expression of VEGF and its receptors in a CDH rat model. However, combined treatment with RA + TO was not superior to either treatment alone.

Citing Articles

Fetoscopic Endoluminal Tracheal Occlusion-Synergic Therapies in the Prenatal Treatment of Congenital Diaphragmatic Hernia.

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Abnormal Fetal Lung of Hoxa1 Piglets Is Rescued by Maternal Feeding with All-Trans Retinoic Acid.

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Prenatal Molecular Hydrogen Administration Ameliorates Several Findings in Nitrofen-Induced Congenital Diaphragmatic Hernia.

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Overexpression of miR-455-5p affects retinol (vitamin A) absorption by downregulating STRA6 in a nitrofen-induced CDH with lung hypoplasia rat model.

Zheng J, He Q, Tang H, Li J, Xu H, Mao X Pediatr Pulmonol. 2020; 55(6):1433-1439.

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miR-455-5p Overexpression Reduces Rat Lung Alveolar Type II Cell Proliferation by Downregulating STRA6.

Zheng J, He Q, Tang H, Xia H Anat Rec (Hoboken). 2019; 302(11):2062-2069.

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