Using Mid Infrared Technology As New Method for the Determination of the Dwell Time of Salivary Substitutes on Three Dimensional Gingiva Models

Overview

Journal BMC Ear Nose Throat Disord

Publisher Biomed Central

Specialty Otorhinolaryngology

Date 2016 Mar 18

PMID 26985168

Citations 2

Authors

Karin Engelhart

Alice Popescu

Jurgen Bernhardt

Affiliations

Soon will be listed here.

Abstract

Background: Many people suffer from dry mouth (xerostomia) due to radiotherapy treatment of head and neck cancer, diseases like Sjogren's syndrome or as adverse effects to prescribed medications. Salivary substitute products like gels or sprays are often used for treatment. Efficacy of those oral care products are regularly assessed by validated or even not validated questionnaires. To determine the adhesion effect over time more objectively a new and sensitive method was established. The following study was designed to assess the dwell time of different oral care products in vitro.

Method: Two different types of surfaces were covered with oral care products and washed using a definite protocol with artificial saliva salt solution. First, oral care gels or oral care sprays were spread to a polystyrene surface of 2.25 cm(2), then onto cell based three-dimensional gingiva models. The surfaces were washed ten times with artificial saliva salt solution. The resulting washing solutions were examined using mid infrared spectroscopy in order to detect ingredients of the oral care products.

Results: All assessed oral care gels or oral care sprays and their components were detected very sensitive. Even traces of the products were detected in the eluent and thus enabled to differentiate the dwell times of the different products. In general, the dwell time of oral care gels on polystyrene or gingiva models was longer than that of oral care sprays. The use of gingiva models improved the differentiation between different products.

Conclusions: MIR spectroscopy turned out to be a sensitive method to detect salivary substitutes. Differences between single components and different products can be detected. The described method is a simple, reliable and easy process to evaluate the dwell time of oral care products in vitro and thus a useful tool to design optimised salivary substitute products.

Ethics: This is an in vitro study. No ethics or consent was required for this study.

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References

Pidgeon C, APOSTOL G, Markovich R . Fourier transform infrared assay of liposomal lipids. Anal Biochem. 1989; 181(1):28-32. DOI: 10.1016/0003-2697(89)90389-8. View

Davies A, Shorthose K . Parasympathomimetic drugs for the treatment of salivary gland dysfunction due to radiotherapy. Cochrane Database Syst Rev. 2007; (3):CD003782. DOI: 10.1002/14651858.CD003782.pub2. View

Momm F, Volegova-Neher N, Schulte-Monting J, Guttenberger R . Different saliva substitutes for treatment of xerostomia following radiotherapy. A prospective crossover study. Strahlenther Onkol. 2005; 181(4):231-6. DOI: 10.1007/s00066-005-1333-7. View

Silvestre F, Minguez M, Sune-Negre J . Clinical evaluation of a new artificial saliva in spray form for patients with dry mouth. Med Oral Patol Oral Cir Bucal. 2008; 14(1):E8-E11. View

Strug I, Utzat C, Cappione 3rd A, Gutierrez S, Amara R, Lento J . Development of a univariate membrane-based mid-infrared method for protein quantitation and total lipid content analysis of biological samples. J Anal Methods Chem. 2014; 2014:657079. PMC: 4211209. DOI: 10.1155/2014/657079. View

Culbert J, Cozzolino D, Ristic R, Wilkinson K . Classification of Sparkling Wine Style and Quality by MIR Spectroscopy. Molecules. 2015; 20(5):8341-56. PMC: 6272211. DOI: 10.3390/molecules20058341. View

Masuch R, Moss D . Stopped flow apparatus for time-resolved Fourier transform infrared difference spectroscopy of biological macromolecules in 1H2O. Appl Spectrosc. 2003; 57(11):1407-18. DOI: 10.1366/000370203322554581. View

Jamme F, Vindigni J, Mechin V, Cherifi T, Chardot T, Froissard M . Single cell synchrotron FT-IR microspectroscopy reveals a link between neutral lipid and storage carbohydrate fluxes in S. cerevisiae. PLoS One. 2013; 8(9):e74421. PMC: 3770668. DOI: 10.1371/journal.pone.0074421. View

Dubois J, Shaw R . IR spectroscopy in clinical and diagnostic applications. Anal Chem. 2004; 76(19):360A-367A. DOI: 10.1021/ac041647v. View

10.

Kong J, Yu S . Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochim Biophys Sin (Shanghai). 2007; 39(8):549-59. DOI: 10.1111/j.1745-7270.2007.00320.x. View

11.

Sarapur S, Shilpashree H . Salivary Pacemakers: A review. Dent Res J (Isfahan). 2013; 9(Suppl 1):S20-5. PMC: 3692195. View

12.

Max J, Chapados C . Glucose and fructose hydrates in aqueous solution by IR spectroscopy. J Phys Chem A. 2007; 111(14):2679-89. DOI: 10.1021/jp066882r. View

13.

Van Eerdenbrugh B, Taylor L . Application of mid-IR spectroscopy for the characterization of pharmaceutical systems. Int J Pharm. 2010; 417(1-2):3-16. DOI: 10.1016/j.ijpharm.2010.12.011. View

14.

Wang L, Mizaikoff B . Application of multivariate data-analysis techniques to biomedical diagnostics based on mid-infrared spectroscopy. Anal Bioanal Chem. 2008; 391(5):1641-54. DOI: 10.1007/s00216-008-1989-9. View

15.

Hahnel S, Behr M, Handel G, Burgers R . Saliva substitutes for the treatment of radiation-induced xerostomia--a review. Support Care Cancer. 2009; 17(11):1331-43. DOI: 10.1007/s00520-009-0671-x. View

16.

Cozzolino D, Cynkar W, Shah N, Smith P . Technical solutions for analysis of grape juice, must, and wine: the role of infrared spectroscopy and chemometrics. Anal Bioanal Chem. 2011; 401(5):1475-84. DOI: 10.1007/s00216-011-4946-y. View

17.

Furness S, Bryan G, McMillan R, Worthington H . Interventions for the management of dry mouth: non-pharmacological interventions. Cochrane Database Syst Rev. 2013; (8):CD009603. DOI: 10.1002/14651858.CD009603.pub2. View

18.

Dixit V, Cho B, Obendorf K, Tewari J . Identifications of household's spores using mid infrared spectroscopy. Spectrochim Acta A Mol Biomol Spectrosc. 2014; 123:490-6. DOI: 10.1016/j.saa.2013.11.053. View

19.

Petersen P . Oral cancer prevention and control--the approach of the World Health Organization. Oral Oncol. 2008; 45(4-5):454-60. DOI: 10.1016/j.oraloncology.2008.05.023. View

20.

Proctor G, Carpenter G . Regulation of salivary gland function by autonomic nerves. Auton Neurosci. 2006; 133(1):3-18. DOI: 10.1016/j.autneu.2006.10.006. View