Message Amplification Phenotyping (MAPPing): a Technique to Simultaneously Measure Multiple MRNAs from Small Numbers of Cells

Overview

Journal Biotechniques

Specialties Biomedical Engineering
Biotechnology

Date 1989 Nov 1

PMID 2698190

Citations 55

Authors

C A Brenner

A W Tam

P A Nelson

E G Engleman

N Suzuki

K E FRY

J W Larrick

Affiliations

Soon will be listed here.

Abstract

A rapid and highly sensitive technique (MAPPing: message amplification phenotyping) has been developed to simultaneously analyze the array of messenger RNAs made by small numbers of cells. The technique incorporates a micro-procedure for isolating RNA, reverse transcription of total cellular RNA to produce cDNA, and enzymatic amplification of cytokine-specific DNA fragments using the polymerase chain reaction. In this study, the technique has been applied to the analysis of cytokines produced by lymphoid cells ranging in number from a single cell to 10(6) cells. The technique should be applicable to virtually any tissue or cell type.

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