Immunological Properties of Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Mar 16

PMID 26977925

Citations 5

Authors

Zhenyu Wang

Qingjun Zhou

Haoyun Duan

Yao Wang

Muchen Dong

Weiyun Shi

Affiliations

Soon will be listed here.

Abstract

Transplantation of ex vivo expanded corneal limbal stem cells (LSCs) has been the main treatment for limbal stem cell deficiency, although the shortage of donor corneal tissues remains a major concern for its wide application. Due to the development of tissue engineering, embryonic stem cells (ESCs)-derived corneal epithelial-like cells (ESC-CECs) become a new direction for this issue. However, the immunogenicity of ESC-CECs is a critical matter to be solved. In the present study, we explored the immunological properties of ESC-CECs, which were differentiated from ESCs. The results showed that ESC-CECs had a similar character and function with LSCs both in vitro and in vivo. In ESC-CECs, a large number of genes related with immune response were down-regulated. The expressions of MHC-I, MHC-II, and co-stimulatory molecules were low, but the expression of HLA-G was high. The ESC-CECs were less responsible for T cell proliferation and NK cell lysis in vitro, and there was less immune cell infiltration after transplantation in vivo compared with LSCs. Moreover, the immunological properties were not affected by interferon-γ. All these results indicated a low immunogenicity of ESC-CECs, and they can be promising in clinical use.

Citing Articles

Transcriptomic Landscape and Functional Characterization of Human Induced Pluripotent Stem Cell-Derived Limbal Epithelial Progenitor Cells.

Polisetti N, Rapp J, Liang P, Dettmer-Monaco V, Bucher F, Pruszak J Cells. 2022; 11(23).

PMID: 36497012 PMC: 9737332. DOI: 10.3390/cells11233752.

Corneal epithelial differentiation of human pluripotent stem cells generates ABCB5 and ∆Np63α cells with limbal cell characteristics and high wound healing capacity.

Vattulainen M, Ilmarinen T, Viheriala T, Jokinen V, Skottman H Stem Cell Res Ther. 2021; 12(1):609.

PMID: 34930437 PMC: 8691049. DOI: 10.1186/s13287-021-02673-3.

Corneal cell therapy: with iPSCs, it is no more a far-sight.

Chakrabarty K, Shetty R, Ghosh A Stem Cell Res Ther. 2018; 9(1):287.

PMID: 30359313 PMC: 6202849. DOI: 10.1186/s13287-018-1036-5.

Universal Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells for Cellularization of a Corneal Scaffold.

Yang J, Park J, Zheng D, Xu R Transl Vis Sci Technol. 2018; 7(5):23.

PMID: 30323996 PMC: 6181193. DOI: 10.1167/tvst.7.5.23.

Long-term homeostasis and wound healing in an in vitro epithelial stem cell niche model.

Miyashita H, Niwano H, Yoshida S, Hatou S, Inagaki E, Tsubota K Sci Rep. 2017; 7:43557.

PMID: 28233843 PMC: 5324070. DOI: 10.1038/srep43557.

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