Characterization and Comparison of Post-natal Rat Achilles Tendon-derived Stem Cells at Different Development Stages

Overview

Journal Sci Rep

Specialty Science

Date 2016 Mar 15

PMID 26972579

Citations 27

Authors

Jialin Chen

Wei Zhang

Zeyu Liu

Ting Zhu

Weiliang Shen

Jisheng Ran

Qiaomei Tang

Xiaonan Gong

Ludvig J Backman

Xiao Chen

Xiaowen Chen

Feiqiu Wen

Hongwei Ouyang

Affiliations

Soon will be listed here.

Abstract

Tendon stem/progenitor cells (TSPCs) are a potential cell source for tendon tissue engineering. The striking morphological and structural changes of tendon tissue during development indicate the complexity of TSPCs at different stages. This study aims to characterize and compare post-natal rat Achilles tendon tissue and TSPCs at different stages of development. The tendon tissue showed distinct differences during development: the tissue structure became denser and more regular, the nuclei became spindle-shaped and the cell number decreased with time. TSPCs derived from 7 day Achilles tendon tissue showed the highest self-renewal ability, cell proliferation, and differentiation potential towards mesenchymal lineage, compared to TSPCs derived from 1 day and 56 day tissue. Microarray data showed up-regulation of several groups of genes in TSPCs derived from 7 day Achilles tendon tissue, which may account for the unique cell characteristics during this specific stage of development. Our results indicate that TSPCs derived from 7 day Achilles tendon tissue is a superior cell source as compared to TSPCs derived from 1 day and 56 day tissue, demonstrating the importance of choosing a suitable stem cell source for effective tendon tissue engineering and regeneration.

Citing Articles

Advancements in Therapeutic Approaches for Degenerative Tendinopathy: Evaluating Efficacy and Challenges.

Morya V, Shahid H, Lang J, Kwak M, Park S, Noh K Int J Mol Sci. 2024; 25(21).

PMID: 39519397 PMC: 11545934. DOI: 10.3390/ijms252111846.

Controlled TPCA-1 delivery engineers a pro-tenogenic niche to initiate tendon regeneration by targeting IKKβ/NF-κB signaling.

Chen J, Sheng R, Mo Q, Backman L, Lu Z, Long Q Bioact Mater. 2024; 44:319-338.

PMID: 39512422 PMC: 11541688. DOI: 10.1016/j.bioactmat.2024.10.016.

Insulin-like growth factor-1 infusion in preterm piglets does not affect growth parameters of skeletal muscle or tendon tissue.

Tangbjerg M, Damgaard A, Karlsen A, Svensson R, Schjerling P, Gelabert-Rebato M Exp Physiol. 2024; 109(9):1529-1544.

PMID: 38980930 PMC: 11363143. DOI: 10.1113/EP092010.

Characterization of TGFβ1-induced tendon-like structure in the scaffold-free three-dimensional tendon cell culture system.

Koo B, Lee Y, Park N, Heo S, Hudson D, Fernandes A Sci Rep. 2024; 14(1):9495.

PMID: 38664570 PMC: 11045825. DOI: 10.1038/s41598-024-60221-4.

Three-Dimensional Cell Culture System for Tendon Tissue Engineering.

Son Y, Yang D, Uricoli B, Park S, Jeong G, Chun H Tissue Eng Regen Med. 2023; 20(4):553-562.

PMID: 37278865 PMC: 10313620. DOI: 10.1007/s13770-023-00550-z.

References

Chen X, Yin Z, Chen J, Shen W, Liu H, Tang Q . Force and scleraxis synergistically promote the commitment of human ES cells derived MSCs to tenocytes. Sci Rep. 2012; 2:977. PMC: 3522101. DOI: 10.1038/srep00977. View

Lo D, Zimmermann A, Nauta A, Longaker M, Lorenz H . Scarless fetal skin wound healing update. Birth Defects Res C Embryo Today. 2012; 96(3):237-47. DOI: 10.1002/bdrc.21018. View

Yin Z, Chen X, Zhu T, Hu J, Song H, Shen W . The effect of decellularized matrices on human tendon stem/progenitor cell differentiation and tendon repair. Acta Biomater. 2013; 9(12):9317-29. DOI: 10.1016/j.actbio.2013.07.022. View

Kohler J, Popov C, Klotz B, Alberton P, Prall W, Haasters F . Uncovering the cellular and molecular changes in tendon stem/progenitor cells attributed to tendon aging and degeneration. Aging Cell. 2013; 12(6):988-99. PMC: 4225469. DOI: 10.1111/acel.12124. View

Tang Q, Chen J, Shen W, Yin Z, Liu H, Fang Z . Fetal and adult fibroblasts display intrinsic differences in tendon tissue engineering and regeneration. Sci Rep. 2014; 4:5515. PMC: 4080701. DOI: 10.1038/srep05515. View

Fang Z, Zhu T, Shen W, Tang Q, Chen J, Yin Z . Transplantation of fetal instead of adult fibroblasts reduces the probability of ectopic ossification during tendon repair. Tissue Eng Part A. 2014; 20(13-14):1815-26. PMC: 4086799. DOI: 10.1089/ten.TEA.2013.0296. View

Havis E, Bonnin M, Olivera-Martinez I, Nazaret N, Ruggiu M, Weibel J . Transcriptomic analysis of mouse limb tendon cells during development. Development. 2014; 141(19):3683-96. DOI: 10.1242/dev.108654. View

Liu H, Zhang C, Zhu S, Lu P, Zhu T, Gong X . Mohawk promotes the tenogenesis of mesenchymal stem cells through activation of the TGFβ signaling pathway. Stem Cells. 2014; 33(2):443-55. DOI: 10.1002/stem.1866. View

Chen J, Zhang W, Liu Z, Heng B, Ouyang H, Dai X . Physical regulation of stem cells differentiation into teno-lineage: current strategies and future direction. Cell Tissue Res. 2015; 360(2):195-207. DOI: 10.1007/s00441-014-2077-4. View

10.

Ezura Y, Chakravarti S, Oldberg A, Chervoneva I, Birk D . Differential expression of lumican and fibromodulin regulate collagen fibrillogenesis in developing mouse tendons. J Cell Biol. 2000; 151(4):779-88. PMC: 2169450. DOI: 10.1083/jcb.151.4.779. View

11.

Irizarry R, Bolstad B, Collin F, Cope L, Hobbs B, Speed T . Summaries of Affymetrix GeneChip probe level data. Nucleic Acids Res. 2003; 31(4):e15. PMC: 150247. DOI: 10.1093/nar/gng015. View

12.

Beredjiklian P, Favata M, Cartmell J, Flanagan C, Crombleholme T, Soslowsky L . Regenerative versus reparative healing in tendon: a study of biomechanical and histological properties in fetal sheep. Ann Biomed Eng. 2003; 31(10):1143-52. DOI: 10.1114/1.1616931. View

13.

Butler D, Juncosa N, Dressler M . Functional efficacy of tendon repair processes. Annu Rev Biomed Eng. 2004; 6:303-29. DOI: 10.1146/annurev.bioeng.6.040803.140240. View

14.

Harris M, Butler D, Boivin G, Florer J, SCHANTZ E, Wenstrup R . Mesenchymal stem cells used for rabbit tendon repair can form ectopic bone and express alkaline phosphatase activity in constructs. J Orthop Res. 2004; 22(5):998-1003. DOI: 10.1016/j.orthres.2004.02.012. View

15.

Baxter M, Wynn R, Jowitt S, Wraith J, Fairbairn L, Bellantuono I . Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion. Stem Cells. 2004; 22(5):675-82. DOI: 10.1634/stemcells.22-5-675. View

16.

Slate R, Posnick J, Wells M, Goldstein J, Keeley F, Thorner P . Fetal tibial bone healing in utero: the effects of miniplate fixation. Plast Reconstr Surg. 1993; 92(5):874-83. View

17.

Lin K, Posnick J, Al-Qattan M, Vajsar J, Becker L . Fetal nerve healing: an experimental study. Plast Reconstr Surg. 1994; 93(7):1323-33. View

18.

Stolzing A, Scutt A . Age-related impairment of mesenchymal progenitor cell function. Aging Cell. 2006; 5(3):213-24. DOI: 10.1111/j.1474-9726.2006.00213.x. View

19.

Zhang G, Ezura Y, Chervoneva I, Robinson P, Beason D, Carine E . Decorin regulates assembly of collagen fibrils and acquisition of biomechanical properties during tendon development. J Cell Biochem. 2006; 98(6):1436-49. DOI: 10.1002/jcb.20776. View

20.

Favata M, Beredjiklian P, Zgonis M, Beason D, Crombleholme T, Jawad A . Regenerative properties of fetal sheep tendon are not adversely affected by transplantation into an adult environment. J Orthop Res. 2006; 24(11):2124-32. DOI: 10.1002/jor.20271. View