A Direct Quantification Method for Measuring Plasma MicroRNAs Identified Potential Biomarkers for Detecting Metastatic Breast Cancer

Overview

Journal Oncotarget

Specialty Oncology

Date 2016 Mar 12

PMID 26967564

Citations 18

Authors

Qian Zhao

Shengqiong Deng

Guangxue Wang

Cuicui Liu

Lingyu Meng

Shanshan Qiao

Lei Shen

Yue Zhang

Jinhui Lu

Wenshu Li

Yuzhen Zhang

Min Wang

Richard G Pestell

Chunli Liang

Zuoren Yu

Affiliations

Soon will be listed here.

Abstract

Circulating miRNAs are protected from ribonuclease degradation by assembly into microvesicles and exosomes. Releasing miRNAs completely from these particles is the key step to quantify the circulating miRNAs. Currently purified RNA-based quantitative analysis is widely used while it is time and cost consuming with high risk for those circulating miRNAs with low abundance due to partial loss of RNA during the steps of total RNA extraction and small RNA enrichment. Herein, we optimized a simple, effective and time-saving method to directly measure plasma miRNAs without RNA isolation. It is based on complete miRNA release from the protein complexes, followed by miRNA-specific reverse transcription and quantitative real-time PCR amplification. By comparison to the RNA-based approach, the direct quantification method showed more efficiency for circulating miRNA analysis, higher accuracy and specificity. By application of the direct quantification method to clinical samples combined with the RNA-based miRNA screening analysis, upregulation of miR-106a in blood was validated in metastatic breast cancer patients, indicating miR-106a are a potential biomarker for metastatic breast cancer.

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References

Ferracin M, Lupini L, Salamon I, Saccenti E, Zanzi M, Rocchi A . Absolute quantification of cell-free microRNAs in cancer patients. Oncotarget. 2015; 6(16):14545-55. PMC: 4546486. DOI: 10.18632/oncotarget.3859. View

Mandel P, Metais P . Nuclear Acids In Human Blood Plasma. C R Seances Soc Biol Fil. 1948; 142(3-4):241-3. View

Calin G, Sevignani C, Dumitru C, Hyslop T, Noch E, Yendamuri S . Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci U S A. 2004; 101(9):2999-3004. PMC: 365734. DOI: 10.1073/pnas.0307323101. View

Chang E, Lo S . Diagnosis and management of central nervous system metastases from breast cancer. Oncologist. 2003; 8(5):398-410. DOI: 10.1634/theoncologist.8-5-398. View

Katsuda T, Kosaka N, Ochiya T . The roles of extracellular vesicles in cancer biology: toward the development of novel cancer biomarkers. Proteomics. 2013; 14(4-5):412-25. DOI: 10.1002/pmic.201300389. View

Yu Z, Wang C, Wang M, Li Z, Casimiro M, Liu M . A cyclin D1/microRNA 17/20 regulatory feedback loop in control of breast cancer cell proliferation. J Cell Biol. 2008; 182(3):509-17. PMC: 2500136. DOI: 10.1083/jcb.200801079. View

Arroyo J, Chevillet J, Kroh E, Ruf I, Pritchard C, Gibson D . Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci U S A. 2011; 108(12):5003-8. PMC: 3064324. DOI: 10.1073/pnas.1019055108. View

Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K . Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 2008; 18(10):997-1006. DOI: 10.1038/cr.2008.282. View

Kosaka N, Iguchi H, Ochiya T . Circulating microRNA in body fluid: a new potential biomarker for cancer diagnosis and prognosis. Cancer Sci. 2010; 101(10):2087-92. PMC: 11159200. DOI: 10.1111/j.1349-7006.2010.01650.x. View

10.

. Finishing the euchromatic sequence of the human genome. Nature. 2004; 431(7011):931-45. DOI: 10.1038/nature03001. View

11.

Turchinovich A, Samatov T, Tonevitsky A, Burwinkel B . Circulating miRNAs: cell-cell communication function?. Front Genet. 2013; 4:119. PMC: 3695387. DOI: 10.3389/fgene.2013.00119. View

12.

Tili E, Michaille J, Gandhi V, Plunkett W, Sampath D, Calin G . miRNAs and their potential for use against cancer and other diseases. Future Oncol. 2007; 3(5):521-37. DOI: 10.2217/14796694.3.5.521. View

13.

Kosaka N, Izumi H, Sekine K, Ochiya T . microRNA as a new immune-regulatory agent in breast milk. Silence. 2010; 1(1):7. PMC: 2847997. DOI: 10.1186/1758-907X-1-7. View

14.

Yu Z, Willmarth N, Zhou J, Katiyar S, Wang M, Liu Y . microRNA 17/20 inhibits cellular invasion and tumor metastasis in breast cancer by heterotypic signaling. Proc Natl Acad Sci U S A. 2010; 107(18):8231-6. PMC: 2889540. DOI: 10.1073/pnas.1002080107. View

15.

Ho Y, Xu W, Too H . Direct quantification of mRNA and miRNA from cell lysates using reverse transcription real time PCR: a multidimensional analysis of the performance of reagents and workflows. PLoS One. 2013; 8(9):e72463. PMC: 3764000. DOI: 10.1371/journal.pone.0072463. View

16.

Cummins J, Velculescu V . Implications of micro-RNA profiling for cancer diagnosis. Oncogene. 2006; 25(46):6220-7. DOI: 10.1038/sj.onc.1209914. View

17.

Lam N, Rainer T, Chan L, Joynt G, Lo Y . Time course of early and late changes in plasma DNA in trauma patients. Clin Chem. 2003; 49(8):1286-91. DOI: 10.1373/49.8.1286. View

18.

Esquela-Kerscher A, Slack F . Oncomirs - microRNAs with a role in cancer. Nat Rev Cancer. 2006; 6(4):259-69. DOI: 10.1038/nrc1840. View

19.

Asaga S, Hoon D . Direct serum assay for microRNA in cancer patients. Methods Mol Biol. 2013; 1024:147-55. DOI: 10.1007/978-1-62703-453-1_11. View

20.

Lawrie C, Gal S, Dunlop H, Pushkaran B, Liggins A, Pulford K . Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma. Br J Haematol. 2008; 141(5):672-5. DOI: 10.1111/j.1365-2141.2008.07077.x. View